多组学分析磷胁迫下大豆根瘤碳氮代谢途径
- Title:
- Multiomics Analysis of Carbon and Nitrogen Metabolism Pathways in Soybean Nodules under Phosphorus Stress
- 摘要:
- 为解析磷胁迫对大豆根瘤固氮作用的影响,对磷胁迫处理15 d的大豆根瘤进行代谢组学和蛋白质组学检测,分析根瘤中差异代谢物质和差异蛋白及参与的代谢通路。结果表明:大豆根瘤中代谢物质和蛋白上调和下调以响应磷胁迫,参与11个碳代谢途径和19个氮代谢途径,糖类物质下调,参与糖酵解、果糖和甘露糖代谢、半乳糖代谢、淀粉和蔗糖代谢等碳代谢途径,导致根瘤中能量供应不足,进而影响根瘤固氮作用;氨基酸和酶以上调为主,富集在氨基酸代谢、氮代谢、氨酰tRNA生物合成及嘌呤代谢通路,是大豆根瘤响应磷胁迫的重要氮代谢途径;氨基酸上调为TCA循环中间产物提供碳骨架,碳氮代谢协同作用响应磷胁迫对大豆根瘤固氮作用的影响。研究结果为解析磷胁迫影响大豆根瘤固氮作用的生理机制提供理论参考。
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备注/Memo
黑龙江省省属科研院所科研业务费项目(CZKYF20231C011);国家自然科学基金(32001479)。