[1]闫洪涛,刘格,杜文晴,等.不同进化类型大豆品种光合碳代谢关键酶活性研究[J].大豆科学,2024,43(05):616-623.[doi:10.11861/j.issn.10009841.2024.05.0616]
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不同进化类型大豆品种光合碳代谢关键酶活性研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第43卷 期数: 2024年05期 页码: 616-623 栏目: 出版日期: 2024-09-01
Title:
Study on Key Enzyme Activities of Photosynthetic Carbon Metabolism in Soybean Varieties of Different Evolutionary Types
作者:
闫洪涛1; 刘格1; 杜文晴1; 张甜甜1; 崔喜艳2; 陈展宇1
1.吉林农业大学 农学院，吉林 长春 130118； 2.吉林农业大学 生命科学学院，吉林 长春 130118
关键词:
大豆; 光合速率; RuBP羧化酶; 碳代谢关键酶
DOI:
10.11861/j.issn.10009841.2024.05.0616
摘要:
光合碳代谢是大豆生长发育过程中基础的生理代谢途径，对其自身的产量和品质有重要的影响。为了探索大豆品种演化过程中光合特性的变化规律和碳代谢的演化趋势，本研究以野生、半野生、半栽培和栽培大豆品种为试验材料，对不同进化类型大豆光合特性以及光合碳代谢关键酶活性进行研究。结果表明：净光合速率、气孔导度、蒸腾速率、RuBP羧化酶活性和叶绿素含量在所有类型的大豆品种中均呈现单峰曲线变化，均以栽培型最高，最大值在R2期，栽培型净光合速率、叶绿素含量和RuBP羧化酶活性分别为24.2 μmol·m-2·s-1、99.9 mg·g-1和4.5 μmol·g-1·min-1。碳代谢相关酶活性也均呈单峰曲线变化，蔗糖合成酶、蔗糖磷酸合成酶、淀粉合成酶、ADPG焦磷酸化酶和UDPG焦磷酸化酶活性最大值均出现在R2期，栽培型大豆品种的蔗糖合成酶和ADPG焦磷酸化酶活性显著高于其它类型品种，分别较野生、半野生、半栽培品种高203.4%、67.8%、49.2%和192.5%、142.4%和131.1%。各类型大豆叶片碳代谢相关酶活性，在V2和R2期与Pn呈显著或者极显著正相关，在R4、R6和R8期与Pn无显著相关性或呈负相关，随着大豆品种的演化，叶片光合碳同化能力改善，其中RuBP羧化酶、蔗糖合成酶和ADPG焦磷酸化酶活性增强幅度较高。研究结果可为大豆品种选育及高光效利用研究提供理论基础。

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备注/Memo

吉林省自然科学基金（20210101023JC）；吉林省科技厅创新平台（基地）和人才专项（20230508021RC）。

更新日期/Last Update: 1900-01-01