LIU Xin-lei,XUE Yong-guo,TANG Xiao-fei,et al.Analysis of Metabolic Pathway and Genes Relating Somatic Embryo Induction Based on RNA Sequencing in Soybean[J].Soybean Science,2022,41(05):536-545.[doi:10.11861/j.issn.1000-9841.2022.05.0536]
基于转录组测序的大豆体细胞胚诱导关键基因及代谢途径分析
- Title:
- Analysis of Metabolic Pathway and Genes Relating Somatic Embryo Induction Based on RNA Sequencing in Soybean
- Keywords:
- soybean; somatic embryo; RNA-Seq; genes; DEGs; basal metabolic pathway
- 文献标志码:
- A
- 摘要:
- 大豆体细胞胚诱导再生途径是大豆再生体系的重要途径之一,为了挖掘大豆体细胞胚诱导下多个发育阶段不同部位的差异表达基因,进而分析其与诱导率相关的生物学过程,本研究以大豆品种黑农84幼胚子叶诱导体细胞胚产生过程中3个不同阶段(0,15,30 d)的外植体为材料,对幼胚子叶的近轴面、远轴面及体细胞胚进行转录组测序。测序获得大豆体细胞胚诱导相关的差异表达基因(DEGs)。根据GO和KEGG筛选和富集各阶段的差异基因,挖掘体细胞胚诱导相关的重要基因,分析主要生物代谢通路(Pathway)并绘制显著性 Pathway的作用网络,进而分析细胞胚诱导发育过程中的主要代谢通路。结果表明:体细胞胚诱导发育3个阶段的4个比较组中共同上调或下调表达的DEGs有19个,3个比较组中共同上调或下调表达的DEGs有142个,体细胞胚诱导发育3个阶段的DEGs主要集中在激素诱导、光合作用、氧化还原、转录调控和信号传导等生物学过程。合成代谢途径的富集分析发现DEGs主要富集于代谢途径、次生代谢产物的生物合成、植物激素信号转导、核糖体、光合作用途径等生物代谢合成通路。代谢通路互作关系分析表明基础代谢途径处于体细胞胚诱导发育的核心位置。
- Abstract:
- Somatic embryogenesis is one of the most important ways in soybean regeneration system. In order to explore the differentialy expressed genes in different parts of different developmental stages of soybean induced by somatic embryos, and then analyze the biological processes related to the induction rate, we used the explants of the immature embryo cotyledon of soybean cultivar Heinong 84 to induce somatic embryogenesis at three different stages (0, 15 and 30 d), the transcriptome of immature cotyledons was sequenced on the adaxial, abaxial surfaces and somatic embryos. The differentialy expressed genes (DEGs) related to somatic embryogenesis in soybean were obtained by sequencing. We screened and enriched different genes at various stages according to GO and KEGG,excavated important genes related to somatic embryo induction,analyzed the primary biological metabolic pathways of each stage and plot their action network,and then analysis the major metabolic pathways during cell embryo-induced development. The results showed that the three stages of DEGs were mainly in the biological processes of hormone induction, photosynthesis,redox, transcriptional regulation and signal transduction. The enrichment analysis of anabolic pathway showed that DEGs mainly concentrated in metabolic pathway, biosynthesis of secondary metabolites, plant hormone signal transduction, ribosome, photosynthesis pathway and so on. The metabolic pathway interaction suggests that the basal metabolic pathway is at the core of somatic embryogenesis. Keywords:
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备注/Memo
收稿日期:2022-02-27