[1]姚钦,等.不同种植方式下大豆田土壤微生物磷脂脂肪酸特征[J].大豆科学,2015,34(03):442-448.[doi:10.11861/j.issn.1000-9841.2015.03.0442]
　YAO Qin,XU Yan-li,SONG Jie,et al.Characteristics of Phospholipid Fatty Acids of Soil Microorganism under Different Plant Patterns of Soybean[J].Soybean Science,2015,34(03):442-448.[doi:10.11861/j.issn.1000-9841.2015.03.0442]
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不同种植方式下大豆田土壤微生物磷脂脂肪酸特征

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷 期数: 2015年03期 页码: 442-448 栏目: 出版日期: 2015-06-25
Title:
Characteristics of Phospholipid Fatty Acids of Soil Microorganism under Different Plant Patterns of Soybean
作者:
姚钦1;  2; 许艳丽1; 宋洁1;  3; 魏巍1
1.中国科学院 东北地理与农业生态研究所/黑土区农业生态院重点实验室，黑龙江 哈尔滨 150081；
?2.中国科学院大学 研究生院，北京 100049；?
3.东北农业大学 农学院，黑龙江 哈尔滨 150030.
Author(s):
YAO Qin1; 2;  XU Yan-li1;  SONG Jie1; 3;  WEI Wei1
1.Key Laboratory of Mollisols Agroecology/Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin 150081, China;?
2.Graduate University of Chinese Academy of Sciences, Beijing 100049, China;?
3.College of Agriculture, Northeast Agricultural University, Harbin 150030, China
关键词:
大豆; 轮作; 磷脂脂肪酸; 土壤微生物
Keywords:
Soybean;  Rotation;  Phospholipid fatty acid;  Soil microorganism
DOI:
10.11861/j.issn.1000-9841.2015.03.0442
文献标志码:
A
摘要:
磷脂脂肪酸方法（PLFA）可较准确地表达土壤微生物的类群及其生物量，是一种快速、重现性好的分析土壤微生物群落结构多样性方法。以中国科学院海伦农业生态试验站定位试验区大豆20年连作、轮作、迎茬和裸地的耕层土壤为对象，采用PLFA方法在大豆盛花期、鼓粒期和成熟期对不同轮作方式下土壤微生物群落结构进行研究。结果表明：20年连作使土壤微生物总磷脂脂肪酸含量有所增加，尤其是在盛花期，20年连作微生物总磷脂脂肪酸（PLFA）含量为10 327 μg?g-1，显著高于其它轮作方式，20年轮作和迎茬土壤微生物总磷脂脂肪酸含量在大豆3个生育时期变化并不显著。土壤中真菌、细菌和放线菌PLFA含量与总PLFA含量变化基本一致。但是轮作条件下细菌/真菌磷脂脂肪酸比值显著高于其它轮作方式，说明轮作增加了土壤细菌PLFA含量，降低了真菌PLFA含量。对主要PLFAs进行主成分分析，主成分一（PC1）解释了45.5%的变异，主成分二（PC2）解释了20.6%的变异，而且从主成分得分图上可明显将轮作和迎茬与连作和裸地区分开。结果说明20年连作在一定程度上改变了土壤微生物群落结构，但20年轮作土壤生态系统仍然相对较稳定。
Abstract:
Phospholipid fatty acid (PLFA) method can accurately express soil microbial groups and biomass. It is a rapid and reproducible analysis method of soil microbial community structure diversity.Based on the long term experimental field at Hailun Agro-ecosystem Experimental Station of Chinese Academy of Sciences, soil microorganism community structure was studied at the flowering stage, seed-filling stage and maturing stage of soybean using the PLFA method under 20 years soybean continuous cropping, rotational cropping, normal rotation and bare land.The results showed that total PLFAs of soil microorganism increased under 20 years soybean continuous cropping.It was significantly higher than other rotation systems, especially at the flowering stage (10 327 μg?g-1).There were no significant differences in total PLFAs at the flowering stage, seed-filling stage and maturing stage of soybean between rotational cropping and normal rotation.Soil fungal PLFAs, bacterial PLFAs and Actinomycetic PLFAs had the similar trend compared with total PLFAs in the whole growth period of soybean.But bacterial PLFAs/fungal PLFAs under 20 years rotational cropping was significantly higher than other rotation systems. It indicated that rotational cropping could induce the increase of bacterial PLFAs and decrease of fungal PLFAs.Principal component analysis (PCA) was adopted to investigate the main soil microorganism PLFAs-PC1 explained 45.5% of the variation, PC2 explained 20.6% of the variation, which helped successfully distinguish rotational cropping and normal rotation from 20 years soybean continuous cropping and bare land in the principal components scores charts. It indicated that 20 years soybean continuous cropping could change soil microorganism community structure. However, soil ecosystem kept relatively stable under 20 years rotational cropping.

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备注/Memo

基金项目：中国科学院知识创新工程重要方向项目（kzcx2-yw-408-3）。

第一作者简介：姚钦（1987-），男，博士，主要从事土壤微生态研究。E-mail: invite734506@163.com。
通讯作者：许艳丽（1958-），女，研究员，博导，主要从事植物线虫病害、作物病虫害生物生态控制和土壤微生态研究。E-mail: xyll@iga.ac.cn。

更新日期/Last Update: 2015-07-16