[1]吴蕾,马凤鸣,刘成,等.大豆与玉米、小麦、高粱根系分泌物的比较分析[J].大豆科学,2009,28(06):1021-1025,1030.[doi:10.11861/j.issn.1000-9841.2009.06.1021]
　WU Lei,MA Feng-ming,LIU Cheng,et al.Comparative Analysis of Root Exudates in Soybean，Corn，Wheat and Sorghum[J].Soybean Science,2009,28(06):1021-1025,1030.[doi:10.11861/j.issn.1000-9841.2009.06.1021]
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大豆与玉米、小麦、高粱根系分泌物的比较分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第28卷 期数: 2009年06期 页码: 1021-1025,1030 栏目: 出版日期: 2009-12-25
Title:
Comparative Analysis of Root Exudates in Soybean，Corn，Wheat and Sorghum
文章编号:
1000-9841(2009)06-1021-05
作者:
吴蕾; 马凤鸣; 刘成; 李业成; 王婵婵; 王安娜
东北农业大学 农学院，黑龙江 哈尔滨 150030
Author(s):
WU Lei; MA Feng-ming; LIU Cheng; LI Ye-cheng; WANG Chan-chan; WANG An-na
College of Agriculture，Northeast Agricultural University ，Harbin 150030，Heilongjiang，China
关键词:
大豆; GC-MS; 根系分泌物; 化感物质
Keywords:
Soybean; GC-MS; Root exudates; Allelopathic compounds
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.06.1021
文献标志码:
A
摘要:
为研究大豆根系分泌物在大豆连作障碍中的作用，以二氯甲烷作为溶剂，提取培养28 d的大豆，小麦，玉米，高粱的根系分泌物，运用气质连用仪分离鉴定，找出大豆与其它3种作物根系分泌物的差异。结果表明：用该法鉴定出大豆根系分泌物成分77种，小麦97种，玉米84种，高粱80种。4种作物均鉴定出烷烃、醇、酸、酯、苯、酚、萘、酰胺、酮、醛类以及其他类化合物。将4种作物的根系分泌物进行比较，相同成分有丙三醇、2，4-戊二醇、邻苯二甲酸、乙苯、对二甲苯、苯乙烯、4-甲基-2，6-二叔丁基苯酚、甲基酚和一部分烷烃等。二苯呋喃，芴，3-甲基-4-氧-戊酸，2-亚甲基-丁醛，3-羟基-丁醛，己醛，9，10-二氢-11，12-乙酰-9，10-桥亚乙基蒽等是大豆根系分泌物中独有成分，其在大豆生长过程中的化感作用有待进一步研究。
Abstract:
In order to study the function of soybean root exudates in soybean continuous cropping obstacles，compounds extracted with dichloromethane from root exudates of soybean，wheat，corn and sorghum seedlings，were isolated and identified by GC-MS analysis，the different root exudates between soybean and the other three crops were searched.It showed that the composition in the root exudates of soybean，wheat，corn and sorghum were 77，97，84 and 80 species，respectively.Alkanes，alcohols，acids，esters，benzene，phenol，naphthalene，amides，ketones，aldehydes and other chemical compounds were identified in the four crops.The common ingredients were identified in the root exudates of the four crops，such as glycerol，2，4-pentanediol，phthalic acid，ethylbenzene，paraxylene，styrene，butylated hydroxytoluene，methyl alkanes，and a part of alkanes.The specific compounds of soybean root exudates were dibenzofuran，fluorene，3-methyl-4-oxo-pentanoic acid ，2-methylene-butanal，3-hydroxy-butanal，hexana and 9，10-dihydro-11，12-diacetyl-9，10-ethanoanthracene.

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备注/Memo

基金项目：黑龙江省自然科学基金资助项目(ZJN0701)

第一作者简介：吴蕾（1983-）,女，在读硕士,研究方向为作物生理。E-mail:wulei9920022000@yahoo.com.cn。
通讯作者：马凤鸣,教授，博士生导师。E-mail:fengming_ma@sohu.com。

更新日期/Last Update: 2014-09-12