LIU Chun,WANG Xian-sheng,MA Hao.Genetic Improvement on Soybean Seed Storage Proteins[J].Soybean Science,2008,27(05):866-873.[doi:10.11861/j.issn.1000-9841.2008.05.0866]
大豆种子贮藏蛋白遗传改良研究进展
- Title:
- Genetic Improvement on Soybean Seed Storage Proteins
- 文章编号:
- 1000-9841(2008)5-0866-08
- Keywords:
- Soybean; Seed storage protein; Genetic improvement; 7S and 11S; Subunits
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 大豆种子中含有40%左右的蛋白质,是世界重要的植物蛋白来源,7S和11S组分是大豆种子贮藏蛋白的主要构成部分,约占70%。7S组分主要由α'、α、β亚基构成,这三个亚基分别由Cgy1、Cgy2和Cgy3基因控制;11S组分由酸性多肽链和碱性多肽链组成的A1aB1b、A1bB2、A2B1a、A3B4和A5A4B3等五个亚基构成。这些亚基分别由Gy1、Gy2、Gy3、Gy4和Gy5基因控制。大豆种子贮藏蛋白亚基变异类型丰富,除对大豆种质进行筛选外,国外研究者已通过杂交育种和辐射诱变育种培育出了高11S/7S比值的品系。通过外源基因导入和编码大豆贮藏蛋白基因修饰来提高大豆种子蛋白品质的遗传工程方面已经起步。理化诱变也是改良大豆蛋白品质的一条可行途径。所有这些研究对于改良大豆贮藏蛋白的营养价值和加工品质都具有重要意义。
- Abstract:
- Soybean seeds are one of the most-important vegetable protein sources because of their high protein content (about 40%).The storage proteins in soybean seeds consist of two major components,7S globulin (α-conglycinin) and 11S globulin (glycinin),which together account for about 70% of the total seed protein.7S globulin consists of three primary subunits,α’,α and β,α’,α and β are encoded by Cgy1、Cgy2和Cgy3respectively;11S globulin consists of five major subunits which designated as A1aB1b、A1bB2、A2B1a、A3B4和A5A4B3respectively.Each subunit is composed of two parts:an acidic polypeptide and a basic polypeptide,which are linked by a single disulfide bond.The five subunits are encoded by five genes,which are designated as Gy1、Gy2、Gy3、Gy4和Gy5,respectively.There is great genetic diversity of the relative contents of seed storage protein and their subunits in soybean germplasm.In addition to screening soybean germplasm for variation,high 11S/7S ratio lines have been obtained by crossing breeding and radiation induction.The genetic engineering of soybean proteins by introduction of foreign genes into transgenic soybean plants and modification of genes encoding storage proteins of soybeans have been started.Physic-chemic induction is also a feasible method to improve soybean protein quality.All of these are very important to improve the functional and nutritional properties of soybean proteins.
参考文献/References:
[1] Osborne T B.The vegetable proteins[M].Longmans,Green and Co.London.1924.
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备注/Memo
基金项目:引进国际先进农业技术计划(948计划)资助项目(2001-207);高等学校学科创新引智计划资助。