LIU Li-jun,LIN Hao,TANG Xiao-fei,et al.Drought Stress Influence Soybean Yield Morphogenesis in Different Growth Stages[J].Soybean Science,2011,30(03):405-412.[doi:10.11861/j.issn.1000-9841.2011.03.0405]
干旱胁迫对不同生育阶段大豆产量形态建成的影响
- Title:
- Drought Stress Influence Soybean Yield Morphogenesis in Different Growth Stages
- 文章编号:
- 1000-9841(2011)03-0405-08
- Keywords:
- Soybean; Drought stress; Yield
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 在盆栽条件下，以12个大豆品种（系）为材料，分别在V1~V5，R1~R4，R5~R8期进行干旱胁迫处理，并测定了根系形态指标、干物质积累、光合生理指标，农艺性状及产量的变化。结果表明：苗期干旱胁迫对大豆根系形态影响较大，根体积、根径、根系伸长、根系总表面积都有增大的趋势，而其它时期的干旱胁迫均使根形态发育减缓；干旱胁迫使大豆各生育阶段地上和地下干物重明显降低，并增加植株根冠比；不同生育时期干旱胁迫导致大豆叶肉细胞的净光合率（Pn）值降低，苗期气孔导度（Cond）品种间差异明显，花荚期和鼓粒期干旱胁迫使大豆叶片Cond大幅度下降。叶肉细胞间CO2浓度（Ci）和蒸腾速率（Tr）的变化趋势与气孔导度基本一致；水分胁迫导致大豆产量降低，品种间差异显著，不同生育阶段，品种的抗旱性表现不同，全生育时期都抗旱的品种较少。
- Abstract:
- Seasonal drought is a main environmental factor limited soybean yield in northeast China. Our objective was to determine how drought stress imposed on soybean [Glycine max (L.) Merr.] at various growth stages affected plant characteristics. Twelve soybean cultivars were grown under greenhouse conditions and subjected to normal water supply (17%-18%soil-available water) and drought stress (8%-9%soil-available water) during seedling (V1-V5), flowering (R1-R4) and seed filling (R5-R8) stages. Root morphological traits including length, diameter, volume and surface area were all promoted by stress during seedling, while were inhibited by stress in other periods. Dry matter accumulation was decreased and root/shoot ratio was increased by stress in all tested periods. Drought stress at three periods all declined photosynthetic rate(Pn), and stress at flowering and seed-filling declined intercellular CO2 concentration(Ci), transpiration rate(Tr) and stomatal conductance (Cond). The decrease of seed yield caused by stress varied with varieties significantly. The performance of drought tolerance varied with growth periods, only a few cultivars showed resistant to drought stress at all growth stages.
参考文献/References:
[1]刘丽君, 尹田夫, 孟良, 等. 大豆原生质膜及混合细胞器脂肪酸对干旱胁迫的反应[J]. 大豆科学，1991, 10 (1): 46-52. (Liu L J, Yin T F, Meng L, et al. Effect of drought on plasmic membrane and mix organelle fatty acid[J]. Soybean Science, 1991, 10 (1): 46-52.) [2]陈建中，葛水莲，李磊. 作物抗旱基因工程研究进展[J]. 湖北农业科学,2009,48(2): 475-477. (Chen J Z, Ge S L, Li L. Progress of genetic engineering in crop drought tolerance mechanisms[J]. Hubei Agricultural Sciences, 2009,48(2): 475-477.) [3]董钻.大豆产量生理[M].北京: 中国农业出版社, 2000: 46-49.(Dong Z. Soybean yield physiology[M]. Beijing: China Agricultural Press, 2000: 46-49.) [4]李贵全, 李慧峰, 张海燕, 等. 大豆花荚期抗旱性的鉴定与综合评价[J]. 中国生态农业学报，2007,15(6): 96-100. (Li G Q, Li H F, Zhang H Y, et al. Comprehensive evaluation of flowering-podding period drought resistance of soybean[J]. Chinese Journal of Eco-Agriculture, 2007,15(6): 96-100.) [5]李岩, 潘海春, 李德全. 土壤干旱条件下玉米叶片内源激素含量及光合作用的变化[J]. 植物生理学报, 2000, 26(4) :301-305.(Li Y, Pan H C, Li D Q. Changes in contents of endogenous phytohormones and photosynthesis in leaves of maize(Zea mays?L.） in drying soil[J]. Acta Phytophysiologica Sinica, 2000, 26(4):301-305.） [6]李玉梅, 李建英, 王根林, 等. 水分胁迫对大豆幼苗叶片内源激素的影响[J]. 大豆科学,2007, 26(4):627-629,636. (Li Y M, Li J Y, Wang G L, et al. Studies on mechanism of endogenous hormones in soybean seedling under water stress[J]. Soybean Science, 2007, 26(4):627-629,636.) [7]刘华玲, 马欣荣. 植物抗旱分子机理研究进展[J]. 世界科技研究与发展, 2006,28(6): 33-40.(Liu H L, Ma X R. Molecular mechanism of drought resistance in plants[J]. World Sci-Tech R&D, 2006,28(6): 33-40.) [8]刘晓冰, 金剑, 王光华, 等. 行距对大豆竞争有限资源的影响[J]. 大豆科学, 2004,23(3): 215-221. (Liu X B, Jin J, Wang G H, et al. Influences of row-spacing on competing limited resources in soybean[J]. Soybean Science, 2004,23(3): 215-221.) [9]任冬莲, 路贵和, 刘学义. 适用于大豆成苗期抗旱性与根系生长的研究[J]. 中国油料, 1993(1):37-39. (Ren D L,Lu G H, Liu X Y. Studies on drought-resistant and root growth for soybean seedling[J]. Chinese Oil， 1993(1):37-39.) [10]任冬莲, 任天佑, 刘学义,等. 适用于大豆育种应用的抗旱性鉴定技术研究[J]. 华北农学报, 1997,12(1): 61-64. (Ren D L, Ren T Y, Liu X Y, et al. Studies on the identification methods of soybean drought-resistant suitable for soybean breeding[J]. Acta Agriculturae Boreali-Sinica, 1997,12(1): 61-64.） [11]谢甫绨, 董钻, 孙艳环, 等. 不同生育期干旱对大豆生长和产量的影响[J]. 沈阳农业大学学报，1994，25(1): 13-16. (Xie F T, Dong Z, Sun Y H, et al. Effect of drought at different growth stages on growth and yield in soybean[J]. Journal of Shenyang Agricultural University, 1994，25(1): 13-16.) [12]孙义春, 郭有泉, 初元浦. 高寒地区大豆45 cm 双行密植栽培技术模式[J]. 作物杂志, 2007(1): 51-52. (Sun Y C, Guo Y Q, Chu Y P. 45 cm double-row close planting of soybean cultivation practices at Alpine region[J]. Crops, 2007(1): 51-52.) [13]山仑.旱地农业生理生态基础[M]. 北京: 科学出版社: 1998. (Shan L. The basis of physiological and ecological in dryland agriculture[M]. Beijing: Science Press: 1998.) [14]孙鏖, 易自力, 蒋建雄, 等. 植物抗旱基因及其在草坪草中的应用[J]. 生物技术通报,2007(4): 22-26. (Sun A, Yi Z L, Jiang J X, et al. Gene engineering on plant drought-resistance and use in the turf grass[J]. Biotechnology Bulletin,2007(4): 22-26.) [15]史兰波, 李云荫. 水分胁迫对冬小麦幼苗几种生理指标和叶绿体超微结构的影响（简报）[J]. 植物生理学通讯, 1990 (2): 28-31. (Shi L B, Li Y Y. Effects of water stress on several physiological indicators of winter wheat seedlings and chloroplast ultrastructure[J]. Plant Physiology Communications, 1990 (2): 28-31.) [16]唐益苗, 赵昌平, 高世庆, 等. 植物抗旱相关基因研究进展[J]. 麦类作物学报, 2009, 29(1): 166-173. (Tang Y M, Zhao C P, Gao S Q, et al. Advances in genes related to plant drought tolerance[J]. Journal of Triticeae Crops, 2009, 29(1): 166-173.)
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备注/Memo
基金项目：国家科技支撑计划资助项目（2009BADA8B02）；大豆产业技术体系资助项目。