[1]韩丽梅　王树起　鞠会艳　阎飞.吸附树脂提取的大豆根分泌物种类的GC -MS 分析[J].大豆科学,2003,22(04):301-305.[doi:10.11861/j.issn.1000-9841.2003.04.0301]
　Han Limei Wang Shuqi Ju Huiyan Yan Fei.GC-MS ANALYSIS ON THE KINDS OF SOYBEAN ROOT EXUDATESEXTRACTEDWITH ADSORPTION RESIN[J].Soybean Science,2003,22(04):301-305.[doi:10.11861/j.issn.1000-9841.2003.04.0301]
点击复制

吸附树脂提取的大豆根分泌物种类的GC -MS 分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第22卷 期数: 2003年04期 页码: 301-305 栏目: 出版日期: 2003-11-25
Title:
GC-MS ANALYSIS ON THE KINDS OF SOYBEAN ROOT EXUDATESEXTRACTEDWITH ADSORPTION RESIN
文章编号:
1000 -9841(2003)04 -0301 -05
作者:
韩丽梅　王树起　鞠会艳　阎飞
解放军军需大学农副业生产系, 长春130062
Author(s):
Han Limei 　Wang Shuqi 　Ju Huiyan 　Yan Fei
Agronomy Department of the Quartermaster University of PLA , J ilin Changchun 130062
关键词:
大豆; 根分泌物; GC -MS 分析; 化感作用; 化感物质; 吸附树脂
Keywords:
Soybean ; Root exudates ; GC/MS analysis ; Allelopathy ; Allelopathic compounds ; Adsorption resin
分类号:
S 565.1
DOI:
10.11861/j.issn.1000-9841.2003.04.0301
文献标志码:
A
摘要:
采用GC -MS 分析法, 鉴定了XAD -4 吸附树脂连续提取所获得的大豆根分泌物的种类, 并研究了化感物质(邻苯二甲酸)对大豆幼苗生长的化感作用。结果表明:根分泌物连续捕获系统(XAD -4 吸附树脂)获得的根分泌物种类丰富, 主要是长链脂肪酸、苯甲酸及其衍生物, 直链醇、烯醇、酚、酮、苯胺、酯和一些烃类及其衍生物等有机化合物, 其中很多都被报道是化感物质。在一定浓度范围内, 邻苯二甲酸对大豆幼苗生长具有显著的化感抑制作用。表明大豆根分泌物中存在抑制大豆幼苗生长的化感物质, 由此可引发大豆连作障碍。
Abstract:
Soybean root exudates extracted with XAD -4 resin were identified by GC -MS method , and the allelopathyof chemical compounds (1 , 2 -benzenedicarboxylic acid)on soybean seedling were inresti-gated in this study .The resultsshowed that root exudates extracted with XAD -4 resin was identified as long -chain fatty acids , benzoic acids andits derivates , straight -chain alcohol , enol , phenol , ketone , ester and some hydrocarbon , most of which were also allelopathiccompounds reported .In certain range of concentrations , the 1 , 2 -benzenedicarboxylic acid significantly inhibitedthe growth of soybean seedlings .The results showed that soybean root exudates contained allelopathic compounds thatinhibited seed germination and seedlings growth , and this might cause soybean continuous cropping barriers .

参考文献/References:

1 　王震宇, 王英祥, 陈祖仁.重茬大豆生长发育障碍机制初探[ J] .大豆科学, 1991 , 10(1):31 -36.

2 　于广武, 许艳丽, 刘晓冰, 等.大豆连作障碍机制研究初报[ J] .大豆科学, 1993 , 12(3):237-243.
3 　计钟程, 许文芝.重茬大豆减产与土壤环境变化[ J] .大豆科学,1995, 14(4):321-329.
4 　赵淑英, 赵九洲, 陈洁敏, 等.连作对大豆生理生化特性的影响[ J] .大豆科学, 1995 , 14(2):113-118.
5 　王光华, 许艳丽.大豆根浸提液生化它感现象的研究[ A] .见:许艳丽、韩晓增主编, 大豆重迎茬研究[ C] .哈尔滨:哈尔滨工程大学出版社, 1995 , 73-77.
6 　孔垂华, 徐涛, 胡飞, 等.环境胁迫下植物的化感作用及其诱导机制[ J] .生态学报,2000 , 20(9):849-854.
7 　余叔文, 孙文浩.植物的它感作用.见:余叔文、汤章城主编, 植物生理及分子生物学[ M] .北京:科学出版社, 1998, 699-717.
8 　阎飞, 韩丽梅, 杨振明.论大豆连作障碍中有关化感作用(Allelopathy)研究的若干问题[ J] .大豆科学, 2000, 19(3):269-274.
9 　高子勤, 张淑香, 连作障碍与根际微生态研究Ⅰ .根系分泌物及其生态效应[ J] .应用生态学报, 1998 , 9(5):549-554.
10 李绍文.生态生物化学(二):高等植物之间的生化关系[ J] .生态学杂志, 1989 , 8(1):66-70.
11 沈宏, 严小龙.根分泌物研究现状及其在农业与环境领域的应用[ J] .农村生态环境,2000 , 16(3):51-54.
12 Chou CH , .Allelopathic substances and activities of delonix regiaraf[ J] .JChem Ecol , 1992 ,18 :353-367.
13 杜英君, 靳月华.连作大豆植株化感作用的模拟研究[ J] .应用生态学报, 1999 , 10(2)209-212.
14 韩丽梅, 王树起, 鞠会艳等.大豆根分泌物的鉴定及其化感作用的初步研究[ J] .大豆科学,2000 , 19(2):119-125.
15 毛达如.植物营养研究方法[ M] .北京:北京农业大学出版社, 1994 ,12-26.
16 Tang C S , Young C C.Collection and identificationg of allelopathic compoundsfrom the undisturbed root system of bigalta limpograss(Hemarthriaaltissima.)[ J] .Plant Playsiol , 1982 , 69 :155-160.
17 常理文, 余兆楼, 王维通等.尿中有机酸类代谢物的气相色谱-质谱分析[ J] .色谱, 1990 ,8(1):12-15.
18 孙文浩, 余叔文.相生相克效应及其应用[ J] .植物生理学通讯,1992, 28(2):81-87.
19 李扬瑞.植物的生化互作现象[ J] .土壤, 1993(5):248-251 , 259.
20 吴辉, 郑师章.根分泌物及其生态效应[ J] .生态学杂志, 1992 , 11(6):42-47.
21 牟金明, 李万辉.根系分泌物及其作用[ J] .吉林农业大学学报,1996, 18(4)114-118.
22 刘素萍, 扬之为.根系分泌物[ J] .生态农业研究, 1998, 6(2):34-36.
23 阎飞, 韩丽梅, 孙衍, 等.大豆连作土壤中化感物质浸提剂的生物筛选[ J] .吉林农业科学,2000 , 25(1):7-11.
24 鞠会艳, 韩丽梅, 王树起, 等.连作大豆根分泌物对根腐病病原菌的化感作用[ J] .应用生态学报, 2002, 13(6):723-727.

相似文献/References:

[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
　LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(04):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
　LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(04):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
　WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(04):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
　ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(04):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
　LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(04):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
　DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(04):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
　ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(04):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
　GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(04):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
　WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(04):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
　LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(04):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]

备注/Memo

基金项目:国家“ 九五” 科技攻关重中之重项目(G95 -001-05)资助。　

作者简介:韩丽梅(1963-), 女, 博士, 副教授, 主要从事逆境植物营养生态方面的研究。E -mail:caiyunzhuiyue23 @sohu .com

更新日期/Last Update: 2016-12-31