LI Jia-wen,ZHANG Shuai-feng,WU Geng-xue,et al.Bioinformatics Analysis of GmELF3 and Preliminary Study on the Function of GmELF3Ld[J].Soybean Science,2022,41(01):1-011.[doi:10.11861/j.issn.1000-9841.2022.01.0001]
大豆ELF3的生物信息学分析及GmELF3Ld功能初探
- Title:
- Bioinformatics Analysis of GmELF3 and Preliminary Study on the Function of GmELF3Ld
- Keywords:
- soybean; ELF3; bioinformatics; GmELF3Ld; Arabidopsis; transgenesis
- 文献标志码:
- A
- 摘要:
- ELF3是生物节律钟重要的组成部分，在植物的生长发育中发挥重要作用。大豆是光周期敏感的短日作物，含有5个ELF3同源基因，其中GmELF3a/J是引起大豆绿色革命的明星基因，主要调控大豆童期的发育。为分析其他4个GmELF3同源基因的功能，本研究利用MEGA-X、Multalin SOPMA和ExPASy-Prot Param等工具对它们的进化关系、蛋白功能、特性、亚细胞定位及基因表达情况进行生物信息学分析，同时通过转化拟南芥初步研究GmELF3Ld的功能。结果表明：大豆中5个ELF3基因可以分为两类，其中GmELF3a/J、GmELF3b和GmELF3c为A类，GmELF3Ld和GmELF3Le为B类。GmELF3Le的α螺旋含量及不稳定性指数均为最高。另外，这5个蛋白均为亲水性非跨膜蛋白，含有多个丝氨酸和酪氨酸磷酸化位点，为蛋白活性调控提供条件。B类基因表达量远高于A类且表现出明显的营养器官与生殖器官分化，即GmELF3b和GmELF3c基因在花和种子中高表达，GmELF3Ld和GmELF3Le则在叶片中高表达。转基因拟南芥研究结果表明，GmELF3Ld抑制拟南芥开花关键基因FT和CO的表达，延迟拟南芥的花期。本研究结果表明两类GmELF3同源蛋白具有不同的生物学功能，GmELF3Ld延迟拟南芥的花期。
- Abstract:
- ELF3， an indispensable component of the circadian clock， plays an important role in plant growth and development. Soybean， a photoperiod-sensitive short-day crop， contains five ELF3 homolog genes. Of them， GmELF3a/J is a key gene controlling the juvenility and leading to the green revolution in soybean. In order to analyze the function of the other four GmELF3 homologs， and to study the functional differences and genetic molecular mechanism of GmELF3 in depth， we preformed bioinformatical analysis with MEGA-X， Multalin SOPMA， ExPASy-Prot Param and so on to predict the phylogenetics， protein function， protein property， subcelluar location and gene experssion of GmELF3 homologs (GmELF3s). Meanwhile， the function of GmELF3Ld was further preliminerily analyzed in transgenic Arabidopsis. The results showed that these five homologs can be grouped into two groups: GmELF3a/J， GmELF3b and GmELF3c are in group A， and GmELF3Ld and GmELF3Le are in group B. At the same time， GmELF3Le contains the most α-helix and has the highest instablility index. These five homologs are all hydrophilic non-trans-membrane proteins. They contained multiple Ser- and Tyr- phosphorylation sites， which allow the precise regulation of their activities. The expression level of B-group genes was much higher than that of A-group genes， and showed obvious differentiation between vegetative and reproductive organs. Such as GmELF3Ld and GmELF3Le are highly expressed in leaves， while GmELF3b and GmELF3c are highly expressed in flower and seed. The study of transgenic Arabidopsis showed that GmELF3Ld overexpression can inhibit the expression of FT and CO and delay the flowering time. These results demonstrated that these two types of homologous proteins have the different biological functions and GmELF3Ld can delay flowering of Arabidopsis Thaliana.
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备注/Memo
收稿日期：2021-06-13