[1]魏崃,王伟威,李馨园,等.大豆抗腐霉根腐病的生理差异研究[J].大豆科学,2017,36(03):425-429.[doi:10.11861/j.issn.1000-9841.2017.03.0425]
　WEI Lai,WANG Wei-wei,LI Xin-yuan,et al.Physiological Differences in Soybean Resistance to Pythium Root Rot[J].Soybean Science,2017,36(03):425-429.[doi:10.11861/j.issn.1000-9841.2017.03.0425]
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大豆抗腐霉根腐病的生理差异研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第36卷 期数: 2017年03期 页码: 425-429 栏目: 出版日期: 2017-05-20
Title:
Physiological Differences in Soybean Resistance to Pythium Root Rot
作者:
魏崃1; 2; 3; 王伟威1; 2; 3; 李馨园2; 4; 喻德跃3; 刘丽君1; 2
（1.黑龙江省农业科学院 大豆研究所，黑龙江 哈尔滨 150086； 2.国家农作物改良中心哈尔滨分中心 ，黑龙江 哈尔滨 150086； 3.南京农业大学 农学院，江苏 南京 210095； 4.黑龙江省农业科学院 齐齐哈尔分院，黑龙江 齐齐哈尔 161006）
Author(s):
WEI Lai1; 2; 3;  WANG Wei-wei1; 2; 3;  LI Xin-yuan2; 4;  YU De-yue3;  LIU Li-jun1; 2
（1.Soybean Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China； 2.National Harbin Center for Soybean Improvement, Harbin 150086, China; 3.Nanjing Agricultural University, Nanjing 210095, China； 4.Qiqihar Branch of Heilongjiang Academy of Agricultural Sciences，Qiqihar 161006, China）
关键词:
腐霉根腐病; 大豆; 生理
Keywords:
Pythium-Root Rot; Soybean; Physiological differences.
DOI:
10.11861/j.issn.1000-9841.2017.03.0425
文献标志码:
A
摘要:
腐霉根腐病是大豆主要的土传病害，为揭示抗感病大豆品种的生理差异，对不同抗性品种在腐霉病原菌侵染下的大豆下胚轴中丙二醛、苯丙氨酸解氨酶（PAL）、过氧化物酶（POD）、可溶性糖、脯氨酸含量的变化进行测定。结果表明：腐霉菌侵染后，抗病大豆品种下胚轴的丙二醛含量增加不十分明显；可溶性糖含量都是正向增加，苯丙氨酸解氨酶活性，过氧化物酶活性，脯氨酸含量在不同时段都有所增加且抗病品种明显高于感病品种。而感病品种在腐霉菌侵染条件下，大豆下胚轴中丙二醛含量累积较高，可溶性糖在某一时段内有所减少，苯丙氨酸解氨酶活性、过氧化物酶活性，脯氨酸含量增加幅度小于抗病品种，表明抗病品种具有较强的生理生化调节能力。
Abstract:
Pythium root rot is an important soil-born diseases, revealing the physiological differences of susceptible variety has important significance in resistant varieties breeding. Using physiological techniques to detected MDA, solutions of phenylalanine ammonia-lyase (PAL), peroxidase (POD), the change of soluble sugar and proline content in different resistance cultivars under Pythium infection in soybean hypocotyls.Research results showed that after Pythium infection, in resistant cultivars’hypocotyl and malondialdehyde content were not increased obviously in resistant cultivar, and soluble sugar contents were increased. Solution of phenylalanine ammonialyase activity, peroxidase activity, proline contents increased in different phase and significantly higher than these in the susceptible varieties. Susceptible variety after Pythium ultimum infested conditions, malondialdehyde content were high and soluble sugars would reduce in a given period. Solution of phenylalanine ammonialyase activity, peroxidase activity, and proline content were increased, but less than that in resistant varieties, indicated that resistant varieties had strong physiological and biochemical regulation.

参考文献/References:

［1］李宝英,马淑梅.大豆根腐病原菌的种类及抗原筛选［J］. 植物保护学报, 2000, 27(1): 91-92(Li B Y, Ma S M.Pathogens of soybean root rot and screening of resistant sources［J］. Journal of Plant Protection, 2000, 27(1):91-92.)

［2］马淑梅.黑龙江省大豆根腐病主要病原菌种类分布及抗病种质鉴定［J］.中国农学通报, 2012，28（27）：230-235. (Ma S M. Pathogenic pathogen categories distribution and germplasm resistance identification of soybean root rot in Heilongjiang province［J］. Chinese Agricultural Science Bulletin, 2012，28（27）：230-235.)
［3］Thomson T B, Athow K L, Laviolette F A.The effect of temperature on the pathogenicity of Pythium aphanidermatum, P. debaryanum, and P-ultimum-on soybean［J］. Phytopathology 1971,61: 933-935.
［4］Pieczarka D J, Abawi G S. Influence of soil water potential and temperature on severity of Pythium root rot of snap beans［J］. Phytopathology 1978,68:766-772.
［5］Sippell D W, Hall R. Effects of pathogen species, inoculum concentration, temperature, and soil moisture on bean root rot and plant growth［J］.Canadian Journal of Plant Pathology,1982,4(1):1-7.
［6］Lai W, Allen G X, Elroy R C, et al.Pathogenicity of Pythium species in causing seed rot and damping-off in soybean under controlled conditions［J］. Phytoprotection, 2010, 91:3-10.
［7］魏崃，李文滨，韩英鹏,等.大豆品种对四种腐霉菌的抗性研究［J］. 大豆科学, 2010, 29（6）：971-975.(Wei L, Li W B, Han Y P, et al. Identification of resistance to four Pythium species in soybean cultivars［J］. Soybean Science, 2010, 29（6）：971-975.)
［8］郝再彬，荃品，徐伸.植物生理学实验［M］. 北京：中国农业出版社，2004.（Hao Z B, Chang J, Xu S.Plant physiology experiment［M］.Beijing:China Agriculture Press,2004.）
［9］张志良，瞿伟青.《植物生理学实验指导》［M］.北京:高等教育出版社，2006.(Zhang Z L, Zhai W Q. Plant physiology experiment instruction［M］. Beijing:Higher Education Press,2006.)
［10］周研. 盐胁迫对大豆种子萌发，离子平衡及可溶性糖含量影响的研究［D］.长春：东北师范大学，2014.（Zhou Y. Research on germination, ionic balance and soluble sugars contention of Glycine max- under saline stress［D］. Changchun: Northeast Normal University,2014.）
［11］Vogt T.Phenylpropanoid biosynthesis［J］. Molecular Plant,2010,3(1):2-20.
［12］徐鹏飞，常敬礼, 赵福华，等.野生大豆接种大豆疫霉根腐病菌后多酚氧化酶（PPO）活性变化［J］，大豆科学, 2012，31(1)：99-102.(Xu P F, Chang J L, Zhao F H, et al.Response of polyphenol oxidase activity in Glycine soja inoculated with Phytophthora sojae.［J］. Soybean Science,2012, 31(1):99-102.)

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备注/Memo

基金项目：国家现代农业产业技术体系（CARS-04-PS05）；黑龙江省农业科学院创新基金（2014ZD011）；国家转基因重大专项（2016ZX08004-002）。

第一作者简介：魏崃（1985-），女，博士，助理研究员，主要从事大豆遗传育种研究。E-mail：adalwei@126.com。

更新日期/Last Update: 2017-06-28