[1]李雪光,等.大豆种子寄藏真菌致病性测定方法比较[J].大豆科学,2015,34(01):93-98.[doi:10.11861/j.issn.1000-9841.2015.01.0093 ]
　LI Xue-guang,LIU Zhi-yong,ZHAN Li-li,et al.Comparison on Methods for Pathogenicity Test of Seed-Borne Fungi in Soybean[J].Soybean Science,2015,34(01):93-98.[doi:10.11861/j.issn.1000-9841.2015.01.0093 ]
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大豆种子寄藏真菌致病性测定方法比较

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷 期数: 2015年01期 页码: 93-98 栏目: 出版日期: 2015-02-25
Title:
Comparison on Methods for Pathogenicity Test of Seed-Borne Fungi in Soybean
作者:
李雪光1;  2; 刘志勇1;  2; 战丽莉1;  3; 宋洁1; 2; 鲁建聪1;  2; 许艳丽1
1.中国科学院 东北地理与农业生态研究所/黑土区农业生态院重点实验室，黑龙江 哈尔滨 150081；
2.东北农业大学 农学院，黑龙江 哈尔滨 150030；
3.黑河学院 物理化学系，黑龙江 黑河 164300
Author(s):
LI Xue-guang1; 2;  LIU Zhi-yong1; 2;  ZHAN Li-li1; 3;  SONG Jie1; 2; LU Jian-cong1; 2;  XU Yan-li1
1.Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin 150081, China; 2.College of Agronomy, Northeast Agricultural University, Harbin 150030, China; 3. Physical Chemistry Department of Heihe University, Heihe 64300, China
关键词:
种子病害; 寄藏真菌; 大豆; 致病性测定
Keywords:
Seed disease; Seed-borne fungi; Glycine max; Pathogenicity test
分类号:
S435.2
DOI:
10.11861/j.issn.1000-9841.2015.01.0093 
文献标志码:
A
摘要:
寄藏真菌可侵染大豆种子，降低种子的萌芽及活力，影响种子的品质和商品等级。为探讨不同接种方法对寄藏真菌致病性的影响，在实验室条件下比较了种子接种法和植株接种法（切顶端接种法、伤口接种法、切茎接种法和下胚轴接种法）对寄藏真菌致病性的影响。结果表明：各方法中寄藏真菌均对大豆产生致病性，并能显著区分寄藏真菌间致病性差异。其中切顶端接种法相比其他3种植物接种方法更简单有效而准确，而种子接种法更适合在室内进行，且周期短，相比植株接种法方便快捷。
Abstract:
Seed-borne fungi could decrease germination, activity, quality and commercial grade by infecting soybean seed. For discussing the effect of different inoculation methods on pathogenicity of seed-borne fungi, seed inoculation method and plant inoculation methods (apical cut inoculation method,wound inoculation method,stem cut inoculation method and hypocotyl inoculation method) were compared at laboratory condition. The result showed that seed-borne fungi could infect soybean under each inoculation method. The differences of pathogenicity between seed-borne fungi were significantly distinguished by all inoculation methods. Compared with other three plant inoculation methods, apical cut inoculation method was simple, effective and accurate. Seed inoculation method was fit for room test and the test cycle was short. It was also more convenient and efficient than plant inoculation.

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备注/Memo

基金项目：国家“十一五”科技支撑计划（2006BAD21B01-15）。

第一作者简介：李雪光（1989-），女，硕士，主要从事大豆病害研究。E-mail:lixueguang1989518@yeah.net。
通讯作者：许艳丽（1958-），女，博士，研究员，博导，主要从事作物病虫害生物生态控制研究。E-mail：xyll@iga.ac.cn。

更新日期/Last Update: 2015-04-12