MAO Yan-zhi,JIANG Hong-wei,LIU Chun-yan,et al.QTL Mapping and Epistasis Analysis of Pods per Plant and Seeds per Plant with an Advanced Backcross Population[J].Soybean Science,2014,33(04):467-472.[doi:10.11861/j.issn.1000-9841.2014.04.0467]
用高世代回交群体定位大豆荚粒性状的QTL及上位性分析
- Title:
- QTL Mapping and Epistasis Analysis of Pods per Plant and Seeds per Plant with an Advanced Backcross Population
- Keywords:
- Advanced backcross populations; Pods per plant; Seeds per plant; Genetic hitchhiking; QTL mapping
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 为进一步利用野生大豆资源，挖掘大豆荚粒性状关键基因，以黑龙江省大面积种植推广的绥农14为轮回亲本，野生种ZYD00006为供体亲本进行多代回交和自交，得到157株BC3F3株系，对其单株荚数、单株粒数、分枝数、单株粒重和百粒重进行相关性分析，并利用基于混合线性模型的QTL network 2.0软件和遗传搭车原理的卡方测验两种方法进行QTL定位，同时采用QTL network进行了上位性分析。结果表明：单株荚数和单株粒数都与单株粒重、分枝数极显著正相关，与百粒重极显著负相关。用两种方法共检测到4个单株荚数QTL，分别定位在B2，D1a，G和N连锁群上；5个单株粒数QTL，分别定位在F，J，D2和K连锁群上，其中定位在F连锁群上的Satt425~Satt663用两种方法都可以检测到。单株荚数和单株粒数上位性分析均检测到3对互作位点。
- Abstract:
- One hundred and fifty-seven BC3F3 plants were constructed with Suinong 14 as recurrent parent and wild soybean ZYD00006 as donor parent in order to utilize the wild soybean germplasm and find the key genes on pod and seed.Pods per plant,seeds number per plant,branches,seeds weight per plant,and 100-seed weight were investigated.Correlation among those traits were analyzed with SPSS software.QTL and epistasis effects for pods per plant and seeds per plant were test by QTL network software 2.0 and chi-square analysis based on genetic hitchhiking.The result showed that pods per plant and seeds per plant were very significantly positive correlated with seeds weight per plant and branches,very negative correlated with 100-seed weight.A total of 4 QTLs related with pods per plant were detected on B2,D1a,G,and N linkage group with those two methods.Five QTLs related with seeds per plant were tested on F,J,D2,and K linkage group,and the interval Satt425-Satt663 on F linkage group could be detected by two methods.Three interaction locus were test for pods per plant and seeds per plant,respectively.
参考文献/References:
[1]张富厚,郑跃进,侯典云,等.大豆荚粒性状对单株产量的效应分析[J].安徽农业科学，2006，34(15):3632-3633.(Zhang F H,Zheng Y J,Hou D Y,et al.Analysis on the effect of pod traits on yield of soybean[J].Journal of Anhui Agricultural Sciences,2006,34(15):3632-3633.) [2]申忠宝,王建丽,潘多锋,等.大豆单株产量与主要农艺性状的灰色关联度分析[J].中国农学通报,2012,28(33):75-77.(Shen Z B,Wang J L,Pan D F,et al.Grey correlation degree analysis of soybean yield and main agronomic traits[J].Chinese Agricultural Science Bulletin,2012,28(33):75-77.) [3]单彩云,魏玉光,张延军,等.黑龙江省大豆主栽品种主要性状灰色关联度分析[J].大豆科学，2009，28(5):945-948.（Shan C Y,Wei Y G,Zhang Y J,et al.Grey correlation degree analysis of the main traits of main varieties in Heilongjiang[J].Soybean Science ，2009，28(5):945-948.) [4]Sun D,Li W,Zhang Z,et al.Quantitative trait loci analysis for the developmental behavior of soybean(Glycine maxL.Merr.)[J].Theoretical and Applied Genetics，2006，112(4):665-673. [5]Chen Q S,Zhang Z C,Liu C Y,et al.QTL analysis of major agronomic traits in soybean[J].Agricultural Sciences in China，2007，6(4):399-405. [6]黄中文,赵团结,喻德跃,等.大豆产量有关性状QTL的检测[J].中国农业科学,2009,42(12):4155-4165.(Huang Z W,Zhao T J,Yu D Y,et al.QTL mapping for soybean yield traits[J].Scientia Agricultura Sinica,2009,42(12):4155-4165.) [7]Zhang D,Cheng H,Wang H,et al.Identification of genomic regions determining flower and pod numbers development in soybean(Glycine maxL.)[J].Journal of Genetics and Genomics,2010,37(8):545-556. [8]高静瑶,刘春燕,蒋洪蔚,等.多环境下大豆单株荚数性状的QTL分析[J].中国油料作物学报,2012,34(1):1-7.（Gao J Y,Liu C Y,Jiang H W,et al.QTL analysis of pod number per plant in soybean under multiple locations[J].China Jourral of Oil Crop Sciencces,2012,34(1):1-7.） [9]严长杰，顾铭洪.高代回交QTL分析与水稻育种[J].遗传,2000，22(6):419-422.(Yan C J,Gu M H.QTL analysis and genetic breeding of rice in advanced backcross population[J].Genetic,2000,22(6):419-422.) [10]张学勇,童依平,游光霞,等.选择牵连效应分析:发掘重要基因的新思路[J].中国农业科学,2006,39(8):1526-1535.(Zhang X Y,Dong Y P,You G X,et al.Analysis of the hitchhiking effect:to explore new ideas[J].Scientia Agricultura Sinica,2006,39(8):1526-1535.) [11]曾庆力,蒋洪蔚,刘春燕,等.利用高世代回交群体对大豆小粒性状的基因型分析及QTL定位[J].中国油料作物学报,2012,34(5):473-477.(Zeng Q L,Jiang H W,Liu C Y,et al.Genotype analysis and QTL mapping small seed size soybean with advanced backcross population[J].Chinese Journal of Oil Crop Sciences,2012,34(5):473-477.) [12]Lee J D，Yu J K，Hwang Y H，et al.Genetic diversity of wild soybean(Sieb.and Zucc.)accessions from south korea and other countries[J].Crop Science，2008，48(2):606-616. [13]阚贵珍，童振峰，胡振宾,等.野生大豆荚粒相关性状QTL定位[J].大豆科学,2012，31(3):333-340.(Kan G Z,Tong Z F,Hu Z B,et al.QTL analysis on wild soybean pod and seed traits[J].Soybean Science,2012，31(3):333-340.) [14]Sebolt A M，Shoemaker R C，Diers B W.Analysis of a quantitative trait locus allele from wild soybean that increases seed protein concentration in soybean[J].Crop Science，2000，40(5):1438-1444. [15]Tuyen D D，Lal S K，Xu D H.Identification of a major QTL allele from wild soybean(Glycine sojaSieb.& Zucc.)for increasing alkaline salt tolerance in soybean[J].Theoretical and Applied Genetics，2010，121(2):229-236. [16]Wang D，Graef G L，Procopiuk A M，et al.Identification of putative QTL that underlie yield in interspecific soybean backcross populations[J].Theoretical and Applied Genetics，2004，108(3):458-467. [17]朱俊义,杨光宇,赵凤娟,等.野生大豆抗盐解剖结构研究[J].东北师大学报:自然科学版,2004,35(4):105-108.(Zhu J Y,Yang G Y,Zhao F J,et al.Study on resistant salt structure of Glycine soja[J].Journal of Northeast Normal University:Natural Science Edition,2004,35(4):105-108.) [18]郑惠玉，杨光宇，韩春风,等.利用野生大豆(G.soja)种质育成小粒大豆新品种“吉林小粒1号”的选育报告[J].吉林农业科学,1991(3):2.(Zheng H Y,Yang G Y,Han C F,et al.Breeding report on new soybean variety “Jilin xiaoli 1 with wild soybean(G.soja)germplasm[J].Jilin Agricultural Sciences,1991(3):2.) [19]王荣昌.大豆栽培种与野生种种间杂种后代遗传变异研究[J].中国油料,1980(2):41-45.(Wang R C.Study on genetic variation of soybean cultivar and wild interspecific hybrids[J].Chinese Journal of Oil Crop,1980(2):41-45.) [20]蒋洪蔚,刘春燕,高运来,等.作物QTL定位常用作图群体[J].生物技术通报，2008(S1):12-17.(Jiang H W,Liu C Y,Gao Y L,et al.Common mapping population of QTL mapping of crop[J].Biotechnology Bulletin,2008(S1):12-17.) [21]陈冰嬬，石英尧，崔金腾.利用BC2F2高代回交群体定位水稻籽粒大小和形状QTL[J].作物学报,2008,34(8):1299-1307.(Chen B R,Shi Y Y,Cui J T.QTL detection of grain size and shape with BC2F2 advanced backcross population of rice(Oryza sativaL.)[J].Acta Agronomica Sinica,2008,34(8):1299-1307.) [22]蒋洪蔚,李灿东,刘春燕,等.大豆导入系群体芽期耐低温位点的基因型分析及QTL定位[J].作物学报,2009，35(7):1268-1273.(Jiang H W,Li C D,Liu C Y,et al.Genotype analysis and QTL mapping for tolerance to low temperature in germination by introgression lines in soybean[J].Acta Agronomica Sinica,2009，35(7):1268-1273.) [23]董华林,张晨昕,曾波,等.利用野生稻高代回交群体分析水稻农艺性状QTL[J].华中农业大学学报,2009(6):645-650.(Dong H L,Zhang C X,Zeng B,et al.QTL mapping for agronomic trait of rice with wild rice advanced backcross population[J].Journal of Huazhong Agricultural University,2009(6):645-650.) [24]王贤智.大豆产量相关性状的遗传与稳定性分析及QTL定位研究[D].北京:中国农业科学院研究生院，2008.(Wang X Z.Inheritance and stability analysis and QTL mapping of yield related traits in soybean[D].Beijing:Graduate School of Chinese Academy of Agricultural Sciences,2008.) [25]陈庆山,张忠臣,刘春燕,等.大豆主要农艺性状的QTL分析[J].中国农业科学,2007，40(1):41-47.（Chen Q S,Zhang Z C,Liu C Y,et al.QTL mapping for main agricultural traits of soybean[J].Scientia Agricultura Sinica,2007,40(1):41-47.) [26]Zhang D,Cheng H,Wang H,et al.Identification of genomic regions determining flower and pod numbers development in Soybean(Glycine maxL.)[J].Journal of Genetics and Genomics,2010,37:545-556.
备注/Memo
基金项目：教育部新世纪优秀人才支撑计划(NECT-1207-01)；黑龙江省自然科学基金重点项目（ZD201213）；现代农业产业技术体系（CARS-04-02A）；黑龙江省博士后基金（LBH-Z12035）;中国博士后基金（2012M520030）；黑龙江省高校长江后备支持计划项目（2014CJHB004）。