MA Yansong,LIU Xinlei,LUAN Xiaoyan,et al.Identification Efficiency about Resistance to Soybean Cyst Nematode with Relative Molecular Markers in Hybrid Progeny[J].Soybean Science,2014,33(02):173-178.[doi:10.11861/j.issn.1000-9841.2014.02.0173]
大豆胞囊线虫病抗性基因相关分子标记对杂交后代抗性的鉴定效率
- Title:
- Identification Efficiency about Resistance to Soybean Cyst Nematode with Relative Molecular Markers in Hybrid Progeny
- 文章编号:
- 1000-9841(2013)06-0173-06
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 大豆胞囊线虫病的鉴定受条件和方法的制约,一直是大豆抗病资源筛选和抗病品种选育的限制因素之一,与抗病基因紧密连锁的分子标记的发现,为抗病鉴定提供了高效而准确的鉴定方法。以抗大豆胞囊线虫病不同世代的育种分离群体为材料,采用人工接种鉴定和抗病基因型鉴定相结合的方法,探讨了标记基因型在不同分离世代的鉴定效率。结果表明:随着分离群体世代的增加,抗病个体在分离群体中所占比例呈上升趋势,分离世代的卡方测验表明抗病性由2个隐性基因控制;基于抗病基因Rhg1开发的Indel标记rhg1I4和SCN_Res Bridge对表型的检测效率达到88.89%,显著高于Satt309(50.00%);随着育种群体世代的增加,标记检测效率有所提高。不同标记组合并没有提高标记的检测效率。结果证明基于抗病基因序列开发的功能性标记在大豆抗胞囊线虫病育种中有广泛的应用前景。
- Abstract:
- The restriction of identification condition and method is one of the important limiting factors for SCN germplasm screening and breeding.Using molecular marker closely linked with resistant gene is an efficient and accurate method to identify the resistance to SCN.In this study,we adopted several generation of segregation populations derived from hybridization between SCN resistant and susceptible parents to evaluate the identification efficiency of molecular markers.The ratio of individuals resistant to SCN in segregation population enhanced with the generation increasing.Chi-test showed the resistance to SCN was controlled by two recessive genes in segregation population.The detection efficiency of two Indel markers based on Rhg1 sequence,rhg1-I4 and SCN_Res Bridge,was 88.89%,and higher than that of Satt309(50.00%).With the increasing of breeding generation,the molecular markers detecting efficiency improved.The combination between different markers didn’t add more accuracy.We could anticipate that the markers designed by resistant sequence were new pathway to accelerate the SCN resistance breeding.
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备注/Memo
基金项目:黑龙江省自然科学基金(C201020);哈尔滨市科技创新人才研究专项资金项目(2011RFXYN034);国家“十二五”科技支撑计划(2011BAD35B06);黑龙江省农业科技创新工程(2012ZD003)。
第一作者简介:马岩松(1977-),男,在读博士,农艺师,主要从事大豆分子标记辅助选择育种研究。E-mail:mys771007@hotmail.com。
通讯作者:栾晓燕(1964-),女,研究员,主要从事大豆抗病育种工作。E-mail:luanxiaoyan1201@163.com。