ZHANG Xingzheng,WANG Changling,LU Furong,et al.Transformation of LePT1 Gene into Soybean via Agrobacterium mediation[J].Soybean Science,2014,33(01):31-34.[doi:10.11861/j.issn.1000-9841.2014.01.0031]
根癌农杆菌介导大豆转化 LePT1 基因的研究
- Title:
- Transformation of LePT1 Gene into Soybean via Agrobacterium mediation
- 文章编号:
- 10009841（2014）01003104
- Keywords:
- Soybean; Embryos tips; Transformation; Agrobacterium mediation; LePT1
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 采用根癌农杆菌介导大豆胚尖的遗传转化方法将番茄的 LePT1 基因导入辽豆17基因组中，以期获得能够高效利用磷素的转基因大豆新品种。PCR结果表明，T0 和T1 代植株基因组均扩增出1.6 kb的目的条带，测序并进行在线Blast比对表明该条带与 LePT1 基因相同，与试验预期相一致。结果表明 LePT1 基因已成功转入T0 代植株，并在T1 代大豆基因组中稳定遗传。
- Abstract:
- For the sake of cultivating soybean germplasm with higher phosphorus acquisition efficency,a LePT1 gene cloned from tomato genome was transformed into soybean embryos tips via Agrobactium mediation.PCR results showed 1.6 kb band was amplified from T0 and T1 genome respectively,moreover,sequencing and online Blast results indicated that the amplified gene was in accordance with LePT1 .In summary,the LePT1 gene was transformed into T0 plants and inherited steadily in T1 generation.
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备注/Memo
基金项目： 辽宁省科技厅资助项目（201202083）。