[1]何? 琳,刘业丽,裴宇峰,等.?2012年黑龙江垦区大豆参试品系纯度鉴定、分子ID构建及遗传多样性分析[J].大豆科学,2013,32(05):591-595.[doi:10.11861/j.issn.1000-9841.2013.05.0591]
　HE Lin,LIU Ye-li,PEI Yu-feng,et al.Purity Identification,Molecular ID Establishment and Genetic Diversity Analysis of Soybeans Attending Heilongjiang Reclamation Regional Test in 2012[J].Soybean Science,2013,32(05):591-595.[doi:10.11861/j.issn.1000-9841.2013.05.0591]
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?2012年黑龙江垦区大豆参试品系纯度鉴定、分子ID构建及遗传多样性分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第32卷 期数: 2013年05期 页码: 591-595 栏目: 出版日期: 2013-10-25
Title:
Purity Identification,Molecular ID Establishment and Genetic Diversity Analysis of Soybeans Attending Heilongjiang Reclamation Regional Test in 2012
作者:
?何? 琳1; 刘业丽1; 裴宇峰2; 刘春燕1; 韩? 雪1; 蒋洪蔚1; 陈庆山3; 胡国华1
?1.黑龙江省农垦科研育种中心，黑龙江 哈尔滨 150090； 2.黑龙江省农垦总局种子管理处，黑龙江 哈尔滨 150036； 3.东北农业大学 农学院，黑龙江 哈尔滨 150030
Author(s):
?HE Lin1; LIU Ye-li1; PEI Yu-feng2; LIU Chun-yan1; HAN Xue1; JIANG Hong-wei1; CHEN Qing-shan3; HU Guo-hua1
关键词:
大豆; 纯度鉴定; 分子ID; 遗传多样性
Keywords:
Soybean; Identification of purity; Molecular ID; Genetic diversity
DOI:
10.11861/j.issn.1000-9841.2013.05.0591
文献标志码:
A
摘要:
?以2012年黑龙江垦区区域试验的121份大豆品系为材料，选择分布在大豆基因组8个连锁群的13对SSR标记进行纯度分析、分子ID构建及遗传多样性分析。结果表明：121份参试大豆品系纯度分布范围为84.62%～100%，平均纯度为99.24%；利用Sat-092等10对引物可以将121个参试大豆品系区分开，并获得唯一的分子ID；品系间遗传相似系数为0～1.00，平均相似系数为0.3402。结果说明黑龙江垦区2012年参试大豆品系遗传同源性较小，差异性较大。
Abstract:
?Simple sequence repeat(SSR)markers has proved to be effective in seed purity identification and genetic diversity analysis.In this paper,121 soybeans for regional test of Heilongjiang reclamation area in 2012 were used,their purity,molecular ID and genetic diversity were analyzed with 13 SSR primers distributed in 8 linkage groups of soybean genetic map.The purity of these varieties ranged from 84.62% to 100%,and averaged 99.24%.Ten SSR markers including Sat092,Satt138,Satt160,Satt231,Satt233,Satt288,Satt369,Satt442,Satt514 and Satt536,were used to identify the 121 soybeans,then a unique molecule ID was obtained for each line.Genetic similarity coefficients among these lines ranged from 0 to 1.00 and the mean coefficient was 0.340 2.The results indicate the soybeans attending regional test of Heilongjiang reclamation area in 2012 have smaller genetic homology.

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备注/Memo

?国家高技术研究发展计划“863计划”（2012AA101106）；现代农业产业体系建设专项(CARS-04-02A)； 国家“十二五”科技支撑计划(2011BAD35B06-1)

更新日期/Last Update: 2013-11-01