SONG Xian-ping,ZHAO Lin,ZHANG Yan-wei,et al.Bioinformatics Analysis of GmGAI ?Homologous Gene in Soybean Genome[J].Soybean Science,2012,31(06):869-873.[doi:10.3969/j.issn.1000-9841.2012.06.002]
大豆基因组GmGAI同源基因的生物信息学分析
- Title:
- Bioinformatics Analysis of GmGAI ?Homologous Gene in Soybean Genome
- 文章编号:
- 1000-9841(2012)06-0869-05
- Keywords:
- Soybean; GAI; DELLA domain; GRAS domain
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 利用Phytozome数据库、NCBI站和MEGA4.0、ClustalX 软件对大豆(Glycine max)基因组中GAI(GA insensitive)同源基因进行生物信息学分析,发现大豆GAI基因有7个拷贝,都不含有内含子,分别分布于第4、5、6、8、10、11和18号染色体上,它们都属于GRAS家族,都存在DELLA(34-106)和GRAS(158-513)2个结构域。氨基酸序列比对和系统进化树分析表明,大豆GAI的7个拷贝进化距离并不是最近的,而是分别有与之最近的其他物种,推测大豆GAI的7个拷贝在进化的过程中功能上发生了一些变化,对植物的生长发育可能会产生不同的影响。
- Abstract:
- Bioinformatics of GmGAI homologous gone in soybean genome was analyzed using Phytome database,NCBI website,MEGA 4.0 and ClustalX softwares.Seven copies of soybean GmGAI homologs without introns were found,and distributed on chromosome 4,5,6,8,10,11 and 18,respectively.They all belonged to GRAS family and had DELLA and GRAS domain.We blast their amino acid sequence and analyzed their phylogenetic tree with other species,and found the seven copies of soybean GmGAI homologs didn’t clustered together and all had the closest species with themselves.The results suggested that larger change about the function of GmGAIs ?had been occurred during evolution,this may have different influence on plant growth and development of soybean.
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备注/Memo
基金项目:国家自然科学基金(31101169,30971810);转基因生物新品种培育科技重大专项(2011ZX08004-005)。第一作者简介:宋仙萍(1989-),女,在读硕士,研究方向为大豆生物技术。E-mail:xianpingsong@yahoo.com.cn。通讯作者:李文滨(1958-),男,教授,博士生导师,从事大豆遗传育种研究。E-mail:wenbinli@yahoo.com。