[1]李永光,李冬梅,李维娜,等.基于不定芽原位诱导的大豆转基因方法[J].大豆科学,2012,31(02):163-166,172.[doi:10.3969/j.issn.1000-9841.2012.02.001]
 LI Yong-guang,LI Dong-mei,LI Wei-na,et al.A New Soybean Transformation Method Based on Adventitious Shoots Induction[J].Soybean Science,2012,31(02):163-166,172.[doi:10.3969/j.issn.1000-9841.2012.02.001]
点击复制

基于不定芽原位诱导的大豆转基因方法

参考文献/References:

[1]Hinchee M A W,Connor-Wond D D,Newen C A,et al.Production of transgenic soybean plants using Agrobacterium -mediated genetic transfer[J].Biotechnology,1988,6:915-922.

[2]王关林,方宏筠.植物基因工程原理与技术[M].北京:科学出版社,1998.(Wang G L,Fang H J.Plant genetic engineering[M].Beijing:Science Press,1998.)

[3]刘海坤,卫志明.大豆遗传转化研究进展[J].植物生理与分子生物学学报,2005,31(2):126-134.(Liu H K,Wei Z M.Recent advances in soybean genetic transformation[J].Journal of Plant Physiology and Molecular Biology,2005,31(2):126-134.)

[4]李永光,黄文佳,周失,.大豆对草丁膦敏感性研究[J].大豆科学,2011,30(3):749-756.(Li Y G,Huang W J,Zhou S,et al.Sensitivity of soybean to herbicide glufosinate[J].Soybean Science2011,30(3):749-756.)

[5]Jefferson R.Assaying chimeric genes in plants:the GUS gene fusion system[J].Plant Molecular Biology Reports,1987,5:387-405.

[6]Sambrook J,Russell D W.Molecular cloning:a laboratory manua[M].3rd ed.New York:Cold Spring Harbor Lab Press2001.

[7]薛仁镐,谢宏峰.利用改良的草丁膦筛选系统快速而有效筛选转基因大豆[J].大豆科学,2006,25(4):373-378.(Xue R GXie H F.Rapid and efficient selection for transgenic soybean plants with the improved glufosinate selection system[J].Soybean Science,2006,25(4):373-378.)

[8]Butaye K M J,Cammue B P A,Delaur S L,et al.Approaches to minimize variation of transgene expression in plants[J].Molecular Breeding,2005,16:79-91.

[9]孙磊,韩俊友,李宏宇.大豆再生植株抗草铵膦的筛选方法研究[J].大豆科学,2010,29(6):1019-1023.(Sun L,Han J Y,Li H Y.Comparison on methods screening of transgenetic soybean resistant to glufosinate[J].Soybean Science,2010,29(6):1019-1023.)

[10]Zeng P,Vadnais DA,Zhang Z,et al.Refined glufosinate selection in Agrobacterium-mediated transformation of soybean[Glycine max(L.)Merrill][J].Plant Cell Reports,2003,22:478-482.

相似文献/References:

[1]刘章雄,李卫东,孙石,等.1983~2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
 LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(02):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
 LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(02):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
 WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(02):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
 ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(02):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
 LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(02):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
 DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(02):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
 ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(02):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
 GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(02):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
 WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(02):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
 LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(02):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]

备注/Memo

基金项目:抗逆转基因大豆培育专项 (2011ZX08004-002)。

第一作者简介:李永光(1984-),男,博士,助理研究员,研究方向为大豆生物技术。E-mail:yongguangli_0451@yahoo.com.cn。
通讯作者:李文滨(1958-),男,教授,博士生导师,主要从事大豆遗传育种方面的研究。E-mail:wenbinli@neau.edu.cn。

更新日期/Last Update: 2014-08-15