[1]陈秀华,柏锡,潘欣,等.基因大豆的培育及抗虫性检测[J].大豆科学,2009,28(06):959-963.[doi:10.11861/j.issn.1000-9841.2009.06.959]
　CHEN Xiu-hua,BAI Xi,PAN Xin,et al.Cultivation of cry 1Iem Gene Transformed Soybean and Insect Resistant Assay[J].Soybean Science,2009,28(06):959-963.[doi:10.11861/j.issn.1000-9841.2009.06.959]
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基因大豆的培育及抗虫性检测

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第28卷 期数: 2009年06期 页码: 959-963 栏目: 出版日期: 2009-12-25
Title:
Cultivation of cry 1Iem Gene Transformed Soybean and Insect Resistant Assay
文章编号:
1000-9841(2009)06-0959-05
作者:
陈秀华; 柏锡; 潘欣; 翟红; 才华; 纪巍; 李勇; 朱延明
东北农业大学 生命科学学院 植物生物工程研究室，黑龙江 哈尔滨 150030
Author(s):
CHEN Xiu-hua; BAI Xi; PAN Xin; ZHAI Hong; CAI Hua; JI Wei; LI Yong; ZHU Yan-ming
Plant Bioengineering Laboratory，College of Life Sciences，Northeast Agriculture University，Harbin 150030，Heilongjiang，China
关键词:
大豆; cry1Iem基因; 子叶节; 转基因抗性植株; 抗虫性检测
Keywords:
Soybean; cry1Iem gene; Contyledonary nodes; Transformed resistant plants; Insect resistant assay
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.06.959
文献标志码:
A
摘要:
以大豆子叶节为外植体，应用农杆菌介导法，将抗虫（cry1Iem）基因转化大豆，筛选标记为bar基因。经Glufasinate筛选，获得大量抗性植株。对转基因T0、T1、T2代植株进行PCR检测，初步证明cry1Iem基因已经整合到大豆基因组中。对T2代PCR阳性植株幼嫩豆荚，采用圆盘分隔法接入初孵幼虫，进行初步的抗虫性检测，得到1株具有明显抗虫效果和7株抗虫效果较好的转基因植株。
Abstract:
Contyledonary nodes were used as explants，and insect resistant gene cry1Iem was transformed into soybean via Agrobacterium-mediated method.Bar served as selection marker，and glufasinate was used in explants transformation to screen resistant shoots.Lots of resistant plants were obtained.With PCR detection of T0、T1、T2 generation，insect resistant gene cry1Iem was proved to be transformed into soybean preliminarily.Furthermore，immature pods of PCR positive plants in T2 generation and newly hatched larvaes were introduced，and initial insect resistant assay was made by disk method.Result suggested that one transgenic plant had an obvious resistant effect，and another seven plants proved to have some effect.

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备注/Memo

基金项目：国家高技术研究发展计划资助项目（2008AA10Z153）；转基因生物新品种培育重大专项资助项目（2008ZX08004-003）；东北农业大学创新团队资助项目（190214）。

第一作者简介：陈秀华(1983-) ，女，在读硕士，研究方向为植物基因工程与分子生物学。E-mail:cxh150600@126.com。
通讯作者：朱延明，教授，博士生导师。E-mail:ymzhu2001@yahoo.com.cn。

更新日期/Last Update: 2014-09-11