[1]郭茜茜,孙聪姝,刘丽君,等.大豆品质的形成和籽粒糖代谢关键酶活性[J].大豆科学,2009,28(05):846-849.[doi:10.11861/j.issn.1000-9841.2009.05.0846]
　GUO Qian-qian,SUN Cong-shu,LIU Li-jun,et al.Quality Formation and the Activities of Relative Enzymes in Glycometabolism in Soybean Seeds[J].Soybean Science,2009,28(05):846-849.[doi:10.11861/j.issn.1000-9841.2009.05.0846]
点击复制

大豆品质的形成和籽粒糖代谢关键酶活性

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第28卷 期数: 2009年05期 页码: 846-849 栏目: 出版日期: 2009-10-25
Title:
Quality Formation and the Activities of Relative Enzymes in Glycometabolism in Soybean Seeds
文章编号:
1000-9841(2009)05-0846-04
作者:
郭茜茜; 孙聪姝; 刘丽君; 董守坤
东北农业大学 农学院，黑龙江 哈尔滨 150030
Author(s):
GUO Qian-qian; SUN Cong-shu; LIU Li-jun; DONG Shou-kun
Agronomy College，Northeast Agricultural University，Harbin 150030，Heilongjiang，China
关键词:
大豆; 6-磷酸葡萄糖脱氢酶; 异柠檬酸脱氢酶; 异柠檬酸裂解酶
Keywords:
Soybean; Glucose-6-phosphate dehydrogenase; Isocitrate dehydrogenase; Isocitrate lyase
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.05.0846
文献标志码:
A
摘要:
以红丰9、合丰46、黒农38、垦丰5、合丰25和黒农42 6个大豆品种为材料，分析蛋白质和脂肪的含量变化过程。从中选取红丰9、垦丰5和黒农42 3份材料，测定籽粒6-磷酸葡萄糖脱氢酶、异柠檬酸脱氢酶和异柠檬酸裂解酶的活性。旨在了解上游物质和能量调配对大豆品质形成的影响。结果表明：蛋白质和脂肪的积累规律相似。脂肪积累量变化与6-磷酸葡萄糖脱氢酶活性强弱变化趋势相同，但脂肪含量变化较酶活性变化延迟一些。异柠檬酸脱氢酶和异柠檬酸裂解酶活性和变化趋势影响着蛋白质合成和积累。
Abstract:
Six soybean varieties，Hongfeng 9，Hefeng 46，Heinong 38，Kenfeng 5，Hefeng 25 and Heinong 42，were examined in this research to study the accumulation of fat and protein in seeds.We also measured the activities of glucose-6-phosphate dehydrogenase，isocitrate dehydrogenase and isocitrate lyase in the seeds of Hongfeng 9，Kenfeng 5，and Heinong 42.To study the relationship between the protein content，fat content and carbrbon metabolism，and to understand the law of distribution of matter and energy in soybean seed.Results showed that the accumulating of protein was similar to that of oil.The change of fat content was in accordance with the activities of glucose-6-phosphate dehydrogenase，and a slightly earlier than the latter.Activities of isocitrate dehydrogenase and isocitrate lyaseto have a co-effect on the buildup and synthesis of protein.

参考文献/References:

[1]刘丽君.生物技术在大豆品质改良中作用的概况[J].大豆通报，1998，6:22-24.(Liu L J.Overview of biotechnology in quality to improve of soybean[J].Soybean Bulletin，1998，6:22-24.)

[2]王培英，许德春，郭玉虹，等.人工诱变改良大豆品质的研究[J].核农学报，2000，14 (1):21-23.( Wang P Y，Xun D C，Guo Y H，et al.Induced mutation for soybean quality[J].Acta Agriculturae Nucleatae Sinica，2000，14 (1):21-23.)
[3]任波，李毅.大豆种子脂肪酸合成代谢的研究进展[J].分子植物育种，2005，3(3):301-306.(Ren B，Li Y.Research advances on fatty acid biogynthesis metabolism in soybean seed[J].Molecular Plant Breeding，2005，3(3):301-306.)
[4]陈恒鹤，尹丽华，王大秋.大豆蛋白质及脂肪含量的遗传和选择效果研究II.早期世代的变异与遗传选择[J].大豆科学，1991，10 (1):1-9.(Chen H H，Yin L H，Wang D Q.Studres on inheritance and selection effect ofprotein and oil content in soybean Ⅱ.Variability and genetic selection in early generations[J].Soybean Science，1991，10 (1):1-9.)
[4]李群.大豆蛋白质与油份含量基因的SSR标记及初步定位[D].南京:南京农业大学，2004:40-46.(Li Q.Targeting and mapping of protein and oil content related genes by SSR markers in soybean[D].Nanjing Agricultural University，2004:40-46.)
[5]王文真，刘兴媛，曹永生.中国大豆种质资源的蛋白质含量研究[J].中国种业，1998，1:35-37.(Wang W Z，Liu X Y，Cao Y S.Study on protein content of China soybean [J].China Seeds，1998，1:35-37.)
[6]单宏.黑龙江省大豆品质及特征特性的初步分析[J].黑龙江农业科学，1999，3:48-50.(Shan H.Study on quality and characteristic of Heilongjiang soybean[J].Heilongjiang Agricultural Sciences，1999，3:48-50.)[7]陈霞，赵贵兴，张淑华，等.黑龙江省大豆品种蛋白质含量及其组份分析[J].大豆通报，2000，6：19-21.(Chen X，Zhao G X，Zhang S H，et al.Study on protein content and ingredient of Heilongjiang soybean [J].Soybean Bulletin，2000，6:19-21.)
[8]苗兴芬，陈庆山，申洪波，等.种肥对大豆籽粒蛋白质、油份含量形成的影响[J].大豆科学，2004，23(4):311-314.(Miao X F，Chen Q S，Shen H B，et al.Affection seed fertilizer on protein and oil content of soybean seeds[J].Soybean Sciene，2004，23(4):311-314.)
[9]宋启建，盖钧镒，马育华.大豆蛋白质和油分含量生态特点研究[J].大豆科学，1990，9(2):121-128.(Song Q J，Gai J Y，Ma Y H.A study on ecological property of protein content and oil content in soybean[J].Soybean Science，1990，9(2):121-128.)
[10]张恒善，付艳华，孙太石，等.大豆种子脂肪和蛋白质积累规律的研究[J].大豆科学，1993，12(4):296-300.(Zhang H S，Fu Y H，Sun T S ，et al.Study on the rule of accuhiuation of oil and protein soybean seed [J].Soybean Science，1993，12(4):296-300.)
[12]张代军，周顺启，栾怀海，等.高油大豆品种蛋白质和油份积累规律的研究[J].大豆科学，2005，24(4):301-304.(Zhang D J，Zhou S Q，Luan H H，et al. Study on protein and oil accumulation law of high-oil soybean varieties[J].Soybean Science，2005，24(4):301-304.)
[13]李志刚，宋书宏，李瑞平，等.大豆籽粒中干物质、脂肪和蛋白质积累规律的研究[J].作物杂志，2008，4:52-54.(Li Z G，Song S H，Li R P，et al.Study on the accumulation regularity of soybean seeds dry matter，fat and protein[J].Crop Magazine，2008，4:52-54.)
[14]陈俊意.甘蓝型黄籽油菜油分与色素，蛋白质及糖代谢间关系的研究[D].重庆:西南农业大学，2004:13-14，53.(Chen J Y.Study on relation between oil content and pigments protein and sugar in rapeseed(Brassica napus.L)[D].Chongqing:Southwest Agricultural University，2004:13-14，53.)
[15]陈玉萍，刘后利.甘蓝型油菜子油分的积累与某些生理生化变化关系的研究[J].武汉植物学研究，1995，13(3):240-246.(Cheng Y P，Liu H L，Studies on the relationship between oil content and the change of biolocical metabolismin Brassica napus L.seed[J].Journal of Wuhan Botanical Research，1995，13(3):240-246.)
[16]陈俊意.甘蓝型黄籽油菜油分与色素，蛋白质及糖代谢间关系的研究[D].重庆:西南农业大学，2004:18-20.(Chen J Y.Study on relation between oil content and pigments protein and sugar in rapeseed(Brassica napus.L)[D].Chongqing:Southwest Agricultural University，2004:18-20.)
[17]Glock G E，Melean P.Levels of enzymes of the direct oxidative pathway of carbohydrate metabolism in mammalian tissues and tumours[J].Biochemistry，1954，56：171-175.
[18]唐湘如，官春云.作物产量和品质的碳氮及脂肪代谢调控研究进展[J].湖南农业大学学报，1997，23(1)93-103.(Tang X R，Guan C Y.Relationship between activities of several enzymes and the oil，protein and yield of rapeseeds(Brassica nap us) [J].Journal of Hunan Agricultural University，1997，23(1)93-103.)
[19]Munshi S K，Kurnari AniLa.Metabolic relationship of pod wall with lipid biosynthesis in developing mustard seeds[J].Plant Physiology and Biochemistry，1997，24(1):51-57.
[20] Luthra R，Munshi S K，Sukhija P S.Relationship of carbohydrate metabolism with lipid biosynthesis in developing sunflower (Helianthus annulus L.)seeds[J].Plant Physiology，1991，137：312-318.

相似文献/References:

[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
　LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(05):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
　LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(05):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
　WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(05):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
　ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(05):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
　LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(05):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
　DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(05):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
　ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(05):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
　GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(05):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
　WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(05):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
　LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(05):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]

备注/Memo

基金项目：国家“十一五”科技支撑计划资助项目（2006BAD21B01-5，2006BAD21B01-8）;大豆生物学省部共建教育部重点实验室开放基金资助项目（SB08B06）。

作者简介：郭茜茜（1983-），女，硕士研究生，研究方向为作物生理学。E-mail:xxguo19850502@126.com。
通讯作者：刘丽君，副教授，博士。E-mail:Liulijun281021@163.com。

更新日期/Last Update: 2014-09-17