[1]李玉梅,王根林,于洪久,等.大豆杀虫剂氧化乐果降解菌的分离[J].大豆科学,2009,28(01):126-129.[doi:10.11861/j.issn.1000-9841.2009.01.0126]
　LI Yu-mei,WANG Gen-lin,YU Hong-jiu,et al.Separating Bacterium Strains to Degrade Soybean Pesticide Omethoate[J].Soybean Science,2009,28(01):126-129.[doi:10.11861/j.issn.1000-9841.2009.01.0126]
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大豆杀虫剂氧化乐果降解菌的分离

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第28卷 期数: 2009年01期 页码: 126-129 栏目: 出版日期: 2009-02-25
Title:
Separating Bacterium Strains to Degrade Soybean Pesticide Omethoate
文章编号:
1000-9841(2009)01-0126-04
作者:
李玉梅1; 3; 4; 王根林2; 于洪久1; 马凤鸣3; 杨慧4; 杜春梅4
1黑龙江省农业科学院农村能源研究所，黑龙江 哈尔滨 150086；
2黑龙江省农业科学院土肥所，黑龙江 哈尔滨150086；
3东北农业大学农学院，黑龙江 哈尔滨150030；
4黑龙江大学生命科学学院，黑龙江 哈尔滨 150084
Author(s):
LI Yu-mei1; 3; 4; WANG Gen-lin2; YU Hong-jiu1; MA Feng-ming3; YANG Hui4; DU Chun-mei4
1County Resource Research Institute of Heilongjiang Academy of Agricultural Sciences，Harbin 150086；
?2Soil and Fertilizer Research Institute of Heilongjiang Academy of Agricultural Sciences，Harbin 150086；
3Agronomy College of Northeast Agricultural University，Harbin 150010；
?4Life and Science College of Heilongjiang University，Harbin 150084,Heilongjiang,China
关键词:
氧化乐果; 生物降解; 假单胞菌属; 气球菌属
Keywords:
Omethoate; Biodegradation; Psedumonans; Aerocuccos
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.01.0126
文献标志码:
A
摘要:
采用富集驯化培养方法，从哈尔滨农药厂污水处理池的活性污泥中筛选获得2株能彻底降解氧化乐果的菌株。氧化乐果浓度为100 mgL-1时，两株菌在1 d内可完成降解，氧化乐果浓度为400 mgL-1时，两株菌在3 d内可完成降解，浓度在1 000 mgL-1时7 d内可完成降解，浓度达2 000 mgL-1时7 d内降解率可分别达到75.28%和72.42%；当农药浓度较高时，菌株生长速度表现为延后。降解谱实验表明：菌株具有较宽的有机磷农药降解谱；通过生理生化分析及16S同源性分析发现，这两株菌与假单胞菌属和气球菌属极其相似。
Abstract:
In this research，two strains which had the ability to degrade omethoate were isolated from the activated sludge polluted sampled in pesticide factory in Harbin city.The omethoate at the concentration of 100 mgL-1?could be degraded absolutely in 1 d，while the concentration of 200 mgL-1?could be degraded absolutely in 3 d；when the concentration of omethoate were up to 2000 mgL-1，it could be degraded in 7d and the degradation rate was 75.28% and 72.42 % respectively.The growth rate of stains was postponed under higher concentration of pesticides.The research also showed that two strains did well in biodegradation other organ phosphate pesticides.They were identified as Psedumonans and Aerocuccos based on their physiological and biochemical properties and the sequence analysis of their 16SrDNA.

参考文献/References:

[1]柏文琴，何凤琴，邱星辉.有机磷农药生物降解研究进展[J].应用与环境生物学报，2004，10(5)：675-680.(Bo W Q，He F Q，Qiu X H.The researching progress on biodegradation organ phosphorus pesticide[J].Application and Environment Biology Journal，2004，10(5)：675-680.）

[2]石成春，徐升，傅彦斌.氧化乐果曲霉降解特性和中间产物的研究[J].中国环境科学，2004，24(2)：180-183(Shi C C，Xu S，Fu Y B.Researching on characteristics and middle production of aspergilli on biodegradation omethoate[J].China Environment Science，2004，24(2)：180-183.)
[3]沈德新，封志纯，杜江.细菌DNA提取方法比较[J].中原医刊，2006，31(10)：20-22.(Shen D X，Feng Z C，Du J.Comparison on extracting bacterium DNA[J].Central Plain Medicine Journal，2006，31(10)：20-22.)
[4]Mulberry W，Karnes J.Parathion hydrolyses specified by the Flavobacterium pod gene[J].Journal of Bacteriology，1989，6740-6746.
[5] Ramirez M，Carrion Gigabitobi Y，Nava Acampo A.Theoretical approach to the mechanism of biological oxidation of organ phosphorus pesticide[J].Toxicology，2000，149：63-68.
[6] Neck D M.Enzymatic detoxify fiction of waste organ phosphate pesticide[J].Agricultural Food，1980，28：10-11.

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备注/Memo

作者简介：李玉梅（1971-），女,副研究员，博士研究生，主要从事土壤微生物、植物营养等方面的研究。E-mail：liyumeiwxyl@126.com。

更新日期/Last Update: 2014-10-03