[1]郭伟,汪潇淋,王慧,等.GmAGL15基因启动子的克隆及序列分析[J].大豆科学,2008,27(05):727-731.[doi:10.11861/j.issn.1000-9841.2008.05.0727]
　GUO Wei,WANG Xiao-lin,WANG Hui,et al.Cloning and Sequence Analysis of GmAGL15 Gene Promoter from Soybean[J].Soybean Science,2008,27(05):727-731.[doi:10.11861/j.issn.1000-9841.2008.05.0727]
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GmAGL15基因启动子的克隆及序列分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年05期 页码: 727-731 栏目: 出版日期: 2008-10-25
Title:
Cloning and Sequence Analysis of GmAGL15 Gene Promoter from Soybean
文章编号:
1000-9841(2008)05-0727-05
作者:
郭伟; 汪潇淋; 王慧; 喻德跃
南京农业大学国家大豆改良中心，作物遗传与种质创新国家重点实验室，江苏 南京 210095
Author(s):
GUO Wei; WANG Xiao-lin; WANG Hui; YU De-yue
National Center for Soybean Improvement，National Key Laboratory of Crop Genetics and Germplasm Enhancement，Nanjing Agricultural University,Nanjing 210095，Jiangsu，China
关键词:
大豆; GmAGL15; 启动子; 电子克隆; 序列分析
Keywords:
Soybean (Glycine maxL; Merr); GmAGL15;  Promoter;  In silico cloning;  Sequence analysis
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.05.0727
文献标志码:
A
摘要:
为探明大豆GmAGL15基因的表达调控规律，应用电子克隆技术从大豆基因组中克隆了GmAGL15 1000 bp的启动子序列，用PLACE在线启动子预测工具分析表明：该序列含有启动子的一般结构，如TATA-BOX、CAAT-BOX。另外还含有一些顺式作用元件，如调控GmAGL15的组织特异的和特定发育阶段的表达，对胁迫的应答，对光的响应，以及反馈调节，推测大豆GmAGL15基因表达有相应组织特异性，可能受蔗糖、生长素和乙烯等的调控。用FootPrinter和PLACE在线工具对大豆与拟南芥等其他4种植物的同源基因启动子的顺式元件进行比较，发现不同植物的启动子既有保守性，又有多样性，转录因子结合位点的分布相似，但也有区别，暗示了GmAGL15基因表达调控的精确性或多样性。
Abstract:
In order to study GmAGL15 gene expression and regulation in soybean，its1000 bp promoter sequence was cloned from the soybean genome in silico. Promoter sequence analyzed by PLACE showed that it had TATA-box，CAAT-box and some cis-acting elements which could regulate specific expression in different organs and response to stresses，light，and self-feedback. In addition，GmAGL15 may be regulated by sucrose，auxin and ethylene. Comparaison of the promoter of GmAGL15 with homologues from other plants by FootPrinter and PLACE suggested that these promoters had conserved and diverse domains，while the distribution of transcription factor binding sites had similarity and difference. These findings implied the accuracy and diversity of GmAGL15 gene expression and regulation.

参考文献/References:

[1] Riechmann J L，Meyerowitz E M.MADS domain proteins in plant development[J].Biological Chemistry，1997，378(10)：1079-1101.

[2]Parenicova L，Folter S de，Kieffer M，et al.Molecular and phylogenetic analyses of the complete MADS-box transcription factor family in Arabidopsis：New openings to the MADS world[J].Plant Cell，2003，15(7)：1538-1551.
[3] Nam J，Kim J，Lee S，et al.Type I MADS-box genes have experienced faster birth and death evolution than type II MADS-box genes in angiosperms[J].Proceedings of the National Academy of Sciences of the United States of America，2004，101(7)：1910-1915.
[4] Causier B，Kieffer M，Davies B.Plant biology：MADS-Box genes reach maturity[J].Science，2002，296(5566)：275-276.
[5]Irish V F.The evolution of floral homeotic gene function[J].Bioessays，2003，25(7)：637-646.
[6] Zik M，Irish V F.Flower development：Initiation，differentiation，and diversification[J].Annual Reviews in Cell and Developmental Biology，2003，19：119-140.
[7]Perry S E，Lehti M D，Fernandez D E.The MADS-domain protein AGAMOUS-like 15 accumulates in embryonic tissues with diverse origins[J].Plant Physiology，1999，120(1)：121-129.
[8]Zhu C，Perry S E.Control of expression and autoregulation of AGL15，a member of the MADS-box family[J].Plant Journal，2005，41(4)：583-594.
[9] Harding E W，Tang W，Nichols K W，et al.Expression and maintenance of embryogenic potential is enhanced through constitutive expression of AGAMOUS-like 15[J].Plant Physiology，2003，133(2)：653-663.
[10]Heck G R，Perry S E，Nichols K W，et al.AGL15，a MADS domain protein expressed in developing embryos[J].Plant Cell，1995，7(8)：1271-1282.
[11]Vandenbussche M，Zethof J，Souer E，et al.Toward the analysis of the petunia MADS box gene family by reverse and forward transposon insertion mutagenesis approaches：B，C，and D floral organ identity functions require SEPALLATA-like MADS box genes in Petunia[J].The Plant Cell，2003，15(11)：2680-2693.
[12]Leseberg C H，Li A L，Kang H，et al.Genome-wide analysis of the MADS-box gene family in Populus trichocarpa[J].Gene，2006，378：84-94.
[13] Thakare D，Tang W，Hill K，et al.The MADS-domain transcriptional regulator AGAMOUS-like 15 promotes somatic embryo development in arabidopsis and soybean[J].Plant Physiology，2008，146(4)：1663-1672.
[14]汪潇琳,陈艳萍,喻德跃.MADS-box基因GmAGL15在大豆种子发育过程中的表达\J].作物学报，2008，34(2)：330-332.(Wang X L，Chen Y P，Yu D Y.Expression of the MADS-box gene GmAGL15 in seed development of soybean[J].Acta Agronomica Sinica,2008，34(2)：330-332.)
[15]Higo K，Ugawa Y，Iwamoto M，et al.Plant cis-acting regulatory DNA elements (PLACE) database[J].Nucleic Acids Research，1999，27(1)：297-300.
[16] Blanchette M，Tompa M.FootPrinter：a program designed for phylogenetic footprinting[J].Nucleic Acids Research，2003，31(13)：3840-3842.
[17] Korbes A P，Droste A.Carbon sources and polyethylene glycol on soybean somatic embryo conversion[J].Pesquisa Agropecuária Brasileira，2005，40(3)：211-216.
[18] Adamczyk B J，Lehti-Shiu M D，Fernandez D E.The MADS domain factors AGL15 and AGL18 act redundantly as repressors of the floral transition in Arabidopsis[J].Plant Journal，2007，50(6)：1007-1019.
[19] Rounsley S D，Ditta G S，Yanofsky M F.Diverse roles for MADS box genes in arabidopsis development[J].Plant Cell，1995，7(8)：1259-1269.
[20] Alvarez-Buylla E R，Liljegren S J，Pelaz S，et al.MADS-box gene evolution beyond flowers：expression in pollen，endosperm，guard cells，roots and trichomes[J].Plant Journal，2000，24(4)：457-466.

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备注/Memo

基金项目：国家自然科学基金资助项目 (30771362)； 国家高技术研究发展计划（863计划） 资助项目 (2006AA10Z1C1)；教育部高等学校引智计划（111计划） 资助项目 (B08025)。

作者简介：郭伟(1981-),男,硕士研究生,研究方向为生物化学及分子生物学。E-mail:thundergw@yahoo.com.cn。
通讯作者：喻德跃，教授,博士生导师。E-mail: dyyu@njau.edu.cn。

更新日期/Last Update: 2014-10-06