大豆C2H2型锌指蛋白转录因子家族全基因组鉴定及表达分析
- Title:
- Genome-wide Identification and Expression Analysis of C2H2 Zinc Finger Protein Gene Family in Soybean
- 摘要:
- 为进一步寻找与大豆产量相关的潜在候选基因，利用生物信息学与转录组学技术，通过拟南芥（Arabidopsis thaliana L.）C2H2型锌指蛋白（C2H2 zinc finger protein）序列BLAST映射，将从大豆（Glycine max）的基因组数据库中筛选出的44个C2H2锌指蛋白家族成员分成11个亚家族，并对其进行系统进化、保守结构域、顺式作用元件、测序数据和转录组数据等相关分析。结果表明：C2H2锌指蛋白家族成员分布于17条大豆染色体中，且每个亚家族的基因结构域较为相近，呈高度保守性。成员启动子部分含有抗逆境胁迫以及激素相关的调控元件。RNA-seq结果显示，有8个基因在不同植物组织内表达差异显著。荧光定量PCR分析表明，3个基因的分析结果为SUPERMAN的转录调节因子，GmC1-1iZFP42为Ln位点的相应基因，参与大豆叶形和籽粒的调控，GmC1-1iZFP12、GmC1-1iZFP30为SUPERMAN基因的转录调节因子，GmC1-1iZFP21可能参与大豆籽粒形成和发育的过程，为调控相关过程的候选基因。本研究为进一步寻找和挖掘大豆高产基因提供方向，也为C2H2型锌指蛋白和生物信息学在植物中的利用提供参考和启示。
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