[1]孙海锋,战勇,林海容,等.花期干旱对不同基因型大豆叶绿素荧光特性的影响[J].大豆科学,2008,27(01):56-60.[doi:10.11861/j.issn.1000-9841.2008.01.0056]
　SUN Hai-feng,ZHAN Yong,LIN Hai-rong,et al.Response of Chlorophyll Fluorescence to Drought Stress at Flowering in Different Soybeans[J].Soybean Science,2008,27(01):56-60.[doi:10.11861/j.issn.1000-9841.2008.01.0056]
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花期干旱对不同基因型大豆叶绿素荧光特性的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年01期 页码: 56-60 栏目: 出版日期: 2008-02-25
Title:
Response of Chlorophyll Fluorescence to Drought Stress at Flowering in Different Soybeans
作者:
孙海锋1; 战勇2; 林海容1; 魏凌基1; 雷明1; 潘秀梅1; 何能1
1 石河子大学农学院/新疆兵团绿洲生态农业实验室，新疆石河子 832003；
2 新疆农垦科学院作物研究所，新疆石河子 832003
Author(s):
SUN Hai-feng1; ZHAN Yong2; LIN Hai-rong1; WEI Lin-ji1; LEI Ming1; PAN Xiu-mei1; and HE Neng1
1 College of Agriculture，Shihezi University/Key Laboratory of Oasis Ecology Agriculture of Xinjiang Bingtuan，Shihezi 832003，Xinjiang；

2 Xinjiang Agricultural Reclamation Academy of Science，Shihezi 832003，Xinjiang，China
关键词:
干旱胁迫; 叶绿素荧光; 大豆; 基因型差异; 抗旱性
Keywords:
Key words： Chlorophyll fluorescence; Drought stress; Soybean; Genotypic difference; Drought-resistant
DOI:
10.11861/j.issn.1000-9841.2008.01.0056
文献标志码:
A
摘要:
利用叶绿素荧光动力学测定技术，测定开花期干旱不同基因型大豆品种叶绿素荧光参数的变化。以3个不同基因型大豆品种为材料，应用OS52FL调制式叶绿素荧光仪研究在开花期干旱胁迫下，各荧光参数的变化及用方差分析进行综合评价。结果表明，大豆开花期受旱后，可变荧光与最大荧光比(Fv/Fm)、可变荧光与初始荧光比(Fv/F0)、非光化学淬灭系数(NPQ)均降低，而电子传递速率（ETR）升高，说明光系统Ⅱ(PSⅡ)受到了伤害，使得PSⅡ原初光能转换效率(Fv/Fm)、PSⅡ潜在活性(Fv/F0)、起光保护作用的热耗散降低，光合电子传递速率升高。且荧光参数之间具有相关性。花期干旱胁迫后，各参数存在基因型差异，新大豆1号在花期干旱条件下，光合机构受破坏较轻，其吸收的光能能较多的用于光化学转化能力，抗旱能力强。因此，花期干旱胁迫下，叶绿素荧光参数的变化与大豆品种抗旱性有关，利用大豆叶绿素荧光特性对干旱胁迫的反应差异鉴定品种抗旱性是可行的。
Abstract:
Agricultural Reclamation Academy of Science，Shihezi 832003，Xinjiang，ChinaAbstract： Known as the rapid non-destructive probe for studying plant photosynthesis，chlorophyll fluorescence analysis has been gradually put into use in studying the effects of stressful environments on plant photosynthesis.To investigate the effect of drought stress at flowering on chlorophyll fluorescence characters of soybean，three soybean genotypes were adopted，and the change of the chlorophyll fluorescence parameters were determined by using OS52FL chlorophyll fluorometer.Under the condition of drought at flowering，the ratio of the variable to maximum fluorescence(Fv/Fm)；the ratio to of variable to minimal fluorescencet (Fv/F0) and non-photochemical quenching coefficient (NPQ) decreased，while the electron transport rate(ETR) increased.The analysis indicated that photosystem II damaged，and primary light energy conversion of PSⅡ(Fv/Fm)，potential activities of PSⅡ(Fv/F0) and heat disseminate which possess photoprotective effect were decreased and photosynthetic electron transport increased，and the chlorophyll fluorescence parameters were closely correlated with each other.The change of chlorophyll fluorescence parameters under drought stress at flowering varied with genotypes，and the damage of photosynthetic apparatus of Xindadou 1 was lighter，so more light energy absorbed by Xindadou 1 was converted into chemical energy and the ability of drought tolerance was stronger.Therefore，it is possible to determine the drought resistance in soybean using chlorophyll fluorescence characters.

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备注/Memo

基金项目：新疆兵团博士基金项目（04BSZJ-01）

作者简介：孙海锋（1979-），女，硕士研究生，研究方向生物技术在遗传育种中的应用。E-mail： 3067196shf@163.com
通讯作者(Corresponding author)：魏凌基，教授。E-mail：wlj_agr@shzu.edu.cn

更新日期/Last Update: 2014-10-09