[1]苏亚芬 　周建东 　方 　云 * 　徐宏丽.酶法亚油酸氢过氧化反应的底物抑制的松弛因子*[J].大豆科学,2005,24(01):52-55.[doi:10.11861/j.issn.1000-9841.2005.01.0052]
　Su YafenZhou JiangdongFang Yun*Xu Hongli.FACTORS RELAXING THE SUBSTRATE INHIBITION IN LIPOXYGENASE- CATALYZINGHYDROPEROXIDATION OF LINOLEIC ACID[J].Soybean Science,2005,24(01):52-55.[doi:10.11861/j.issn.1000-9841.2005.01.0052]
点击复制

酶法亚油酸氢过氧化反应的底物抑制的松弛因子*

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第24卷 期数: 2005年01期 页码: 52-55 栏目: 出版日期: 2005-02-25
Title:
FACTORS RELAXING THE SUBSTRATE INHIBITION IN LIPOXYGENASE- CATALYZINGHYDROPEROXIDATION OF LINOLEIC ACID
作者:
苏亚芬 1 　周建东 2 　方 　云 *1 　徐宏丽 1
(1. 江南大学化学与材料工程学院 ,无锡 214036;2. 杭州油脂化工厂,杭州 311200)
Author(s):
Su Yafen1　Zhou Jiangdong2　Fang Yun*1Xu Hongli1
(1. School of Chemical and Material Engineering , Southern Yangtze University , Wuxi 214036;2. Hangzhou Factory of Oil Chemicals , Hangzhou 311200)
关键词:
大豆脂氧酶 ; 亚油酸 ; 底物抑制效应 ; 松弛 ; 氢过氧化物
Keywords:
Soybean - lipoxygenase ; Linoleic acid ; Substrate inhibition ; Relaxation; Hydroperoxide
DOI:
10.11861/j.issn.1000-9841.2005.01.0052
文献标志码:
A
摘要:
研究了外加因子对大豆脂氧酶好氧催化大豆亚油酸氢过氧化过程中底物抑制的松弛作用。结果表明:稀释剂 DMF 不仅可以解决高底物体系中的黏度问题, 改善底物在体系内的溶解性 ,提高底物与酶的互溶性 ,还可以提高产率;初始体系氧饱和可减少底物的抑制作用 。抗氧剂的加入可减少副反应的发生, 在底物浓度为 125 g /L , pH 9, 10℃,[ E] =3. 4 ×10 5 U /mL , φ(DMF)=7%,15mL(O 2 )/min ,ρ(柠檬酸钠)=0. 005 g /L 的优化条件下,反应开始 15 min 后加ρ(抗氧剂)=0. 08g /L ,总反应时间 90min 的最终产率可高达 88. 62%。
Abstract:
T he relaxation of substrate inhibition from additives in aerobic catalysis of hydroperoxidation ofsoybean linoleic acid by soybean lipoxygenase was studied in this paper. The results show that the additionof DMF can not only overcome the higher viscosity resulting from higher substrate level , improve the sub-strate solubility and enhance the coexistence of substrate with enzyme , but increase the yield of hydroper-oxide (HPOD). The higher initial oxygen is found also favorable to relaxing the excess substrate inhibi-tion. Antioxidant can control the side reaction of carbon- carbon fission of the HPOD and free radical initi-ated polymerisation. Under the optimal conditions of pH 9, 10 ℃,[ E] =3. 4 ×105 U/mL , φ(DMF)=7%,15 mL(O 2 )/min, ρ(sodium citrate)=0. 005 g /L , and 125 g(substrate)/L , 0. 08 g(antioxidant)/L wasadded 15 mins after and up to 88. 62 %HPOD was obtained 90 mins later.

参考文献/References:

1　Gardner Harold W. Lipoxygenase as a versatile biocatalyst[ J] .JAOCS, 1996, 73(11):1347- 1356.

2　 Gargouri M ohamed, Legoy Marie Dorminique. Chemoenzymaticproduction of (+) - coriolic acid from trilinolein:coupled synthe-sis and extraction[ J] . JAOCS, 1997, 74(6):641- 645.
3　 Márczy Judit Sz, Né meth ágnes Sz, Samu Zsuzsanna , et al. Pro-duction of hexanal from hydrolyzed sunflower oil by lipoxy genaseand hydroperoxide lyase enzymes [ J] . Biotechnology Letters ,2002, 24:1673- 1675.
4　蔡琨, 方云, 夏咏梅, 等. 大豆脂肪氧合酶的提取及影响酶活因素的研究[ J] . 林产化学与工业, 2004, 24(2):10 - 15.
5　蔡琨, 方云, 胡学铮, 等. 大豆脂肪氧合酶(LOX)的固定化及增强稳定性[ J] . 精细化工, 2004, 21(4):33- 37.
6　蔡琨, 方云, 夏咏梅, 等. 大豆脂肪氧合酶酶促合成亚油酸氢过氧化物[ J] . 精细化工, 2004.
7　 Jiang Zhem - Yue , Woollard Alison C. S. , Wolff Simmon P. .Lipid hydroperoxide measurement by oxidation of Fe 2 + in thepresence of Xylenol orange comparison with the T BA assay andan iodometric method[ J] . Lipids, 1991, 26(10):853 - 856.
8　 Elsholf M. B. W. , Janssen M. , Veldink G. A. , et al. Biocatalyt-ic large - scale production of 13(S) - hydroperoxy - 9　 (Z), 11(E) - octadecadienoic acid from hydrolyzed safflower oil by acrude soybean - flour extract as lipoxygenase source[ J] . Recl.T rav. Chim. Pavs - Bas. 1996, 115:499 - 504.

备注/Memo

基金项目:江苏省自然科学基金资助项目(BK2004019)

作者简介:苏亚芬(1980 -), 女, 在读硕士生, 研究方向为酶催化。
**通讯作者:方云, E - mail:fangy un57@sina. com T el:0510 - 5867220

更新日期/Last Update: 2016-12-23