|Table of Contents|

Cell Morphological Characteristics Study on Anther Abortion of Soybean Cytoplasmic-Nuclear Sterile Line JLCMS9A(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2022年06期
Page:
672-678
Research Field:
Publishing date:

Info

Title:
Cell Morphological Characteristics Study on Anther Abortion of Soybean Cytoplasmic-Nuclear Sterile Line JLCMS9A
Author(s):
CAO Xia1 ZHOU Lei1 WANG Xiao-dong1 WANG Chun-lei2 YU Jing-hui3 YU Hua-rong1 TANG Yan-mei4 CUI Tian-yu1
(1.College of Agronomy, Inner Mongolia Minzu University, Tongliao 028024, China; 2.Tongliao Academy of Agriculture and Animal Husbandry Sciences, Tongliao 028000, China; 3.Tongliao Agricultural Technology Extension Center,Tongliao 028000,China; 4.Inner Mongolia Linxi County Bureau of Agri-pastoral, Chifeng 025250, China)
Keywords:
soybean cytoplasmic-nuclear sterile line JLCMS9A cell morphology meiosis tapetum
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2022.06.0672
Abstract:
In order to explore the occurrence time and reason of pollen abortion of cytoplasmic-nuclear sterile line JLCMS9A of soybean [Glycine max (L.) Merrill] from cyto-morphological perspective, we used the cytoplasmic-nuclear sterile line JLCMS9A as the test material, and the maintainer line JLCMS9B as control material.In the study, we took FAA solution fixing, phenol fuchsin tableting and paraffin sections method to observe morphology of flower organ, microspores and anther, we also analyzed the variation of the morphology and cytological features of flower organs and microspores in their development process. The results showed that there was a significant difference between JLCMS9A and JLCMS9B in pollen tube length, total of stigma and style, pollen size and stigma surface. Each part of floral organ of the sterile line JLCMS9A was smaller or shorter than the maintainer line JLCMS9B. At the meiosis stage of the pollen mother, it showed a normal split of JLCMS9A at the end of the second meiotic division and formed a trichotomy instead of a tetrad, but paraffin section of anthers showed that the JLCMS9A enabled to perform normal tetrad syndication in the spore-making phase, with the early degradation of the tapetum, normal pollen walls were not formed, and the microspores were deformed, resulting in the formation of hollow, invaginated and inactive pollen, which eventually led to abortion. The result indicated the pollen abortion of cytoplasmic-nuclear sterile line JLCMS9A of soybean was occurred at bud stage which due to the premature degradation of the tapetum.

References:

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Last Update: 2022-11-29