|Table of Contents|

Protective Effect of Daidzein on Senescence of H9c2 Cells Induced by Hydrogen Peroxide(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2022年04期
Page:
472-479
Research Field:
Publishing date:

Info

Title:
Protective Effect of Daidzein on Senescence of H9c2 Cells Induced by Hydrogen Peroxide
Author(s):
WANG Xuan1;  LIU Ya-hui2;  LI Yong3;  JIN Ming1
(1.Medical College,Yanbian University， Yanji 136200， China; 2.The Third Affiliated Hospital of Jinzhou Medical University， Jinzhou 121000， China; 3.Yanbian University Hospital， Yanji 136200， China)
Keywords:
daidzein;  oxidative stress;  cell senescence;  antioxidant;  H2O2;  H9c2 cells
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2022.04.0472
Abstract:
In order to investigate the protective effect of Daidzein (DAI) on H2O2-induced senescence of rat cardiomyocytes and its mechanism， we randomly divided the H9c2 cells into the control group， model group (400 μmol?L-1 of H2O2)， and low， medium and high-dose DAI groups (20， 40 and 80 μmol?L-1 of DAI， respertively). And we observed the cell senescence by senescence-associated β-galactosidase (SA-β-gal) staining. The total antioxidant capacity(T-AOC)， superoxide dismutase (SOD) activity and malondialdehyde(MDA) content were detected by the colorimetric assay. The ROS level was detected by DCFH-DA fluorescence staining. The mitochondrial membrane potential was detected by the Rh123 fluorescence staining. The expressions of p53， p21， kelch-like epichlorohydrin-associated protein-1 (Keap1)， nuclear factor E2-associated factor (Nrf2)， heme oxygenase-1(HO-1) proteins in H9c2 cells were detected with the western blotting method. The results showed that DAI decreased the positive staining of SA-β -gal， down-regulated the expression of p53 and p21 proteins， increased the T-AOC level and SOD activity， decreased the MDA and ROS levels， and maintained the stability of mitochondrial membrane potential in aging H9c2 cells induced by H2O2. DAI inhibited the expression of Keap1 protein， up-regulated Nrf2 and HO-1 protein expression. It is suggested that DAI could delay cellular senescence， enhance cellular antioxidant capacity and inhibit cellular oxidative stress. The mechanism may be related to regulation of Nrf2/HO-1 signaling pathways.

References:

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Last Update: 2022-08-11