|Table of Contents|

Identification of Soybean Leaf Spot Pathogen Epicoccum sorghinum in Beijing(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2022年03期
Page:
308-313
Research Field:
Publishing date:

Info

Title:
Identification of Soybean Leaf Spot Pathogen Epicoccum sorghinum in Beijing
Author(s):
CUI Yu-xing1;  BAI Ying-xue2;  WANG Qiu-shi2;  SHANG Qiao-xia2;  REN Jun-da2;  LI Wei-yu1
(1.College of Plant Science and Technology， Beijing University of Agriculture/Beijing Key Laboratory of New Agricultural Application Technology/National Experimental Teaching Demonstration Center of Plant Production， Beijing 102206， China; 2.Beijing University of Agriculture， College of Bioscience and Resources Environment， The Key Laboratory for Northern Urban Agriculture of Ministry of Agriculture and Rural Affairs， Beijing 102206， China)
Keywords:
soybean;  leaf spot disease;  molecular biology;  Epicoccum sorghinum;  Koch’s postulates
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2022.03.0308
Abstract:
Soybean leaf spot disease occurs with a wide range in Beijing， having a serious impact on soybean production. In order to analyze the causes of soybean leaf spot disease in Beijing， thus providing a theoretical basis for the efficient prevention and control of the disease， in this study， we analyzed the symptoms of the disease in the field， collected the diseased plants and isolated the pathogenic bacteria by tissue separation method， and purified the pathogenic bacteria by single spore separation method on the surface of the agar plate. Then we test the pathogenicity according to Koch postulates and determined the type of pathogenic bacteria by morphology and molecular biology. The results showed， at first the pathogenic bacterial colonies were white or gray-white at the initial stage， and all the pathogenic bacterial colonies were reddish-brown at the later stage. The center of the colonies was orange-red， pink， or white with bulges， and the edges were gray-white in an irregular shape. The conidia was spherical， nearly spherical or oval， with a septum in the middle， mostly in clusters， about 20 μm × 5 μm. Combined with molecular biology result， the pathogen was identified as Epicoccum sorghinum. The E. sorghinum was isolated from soybean for the first time， and it was found that the pathogen was highly pathogenic after re-inoculation.

References:

［1］孙长征. 大豆灰斑病发生规律及防控技术研究[D]. 哈尔滨:黑龙江大学， 2010. (SUN C Z. Study on occurrence regularity and control technology of soybean gray spot disease[D]. Harbin: Heilongjiang University， 2010.)[2]韩新华， 许艳丽， 李春杰， 等. 大豆灰斑病的发生与综合防治[J]. 大豆科技， 2007(3): 14-16. (HAN X H， XU Y L， LI C J， et al. Occurrence and management of frog-eye leaf spot in soybean[J]. Soybean Science & Technology， 2007(3): 14-16.)[3]许伟民， 谢昀烨， 王春伟， 等. 北五味子黑斑病的药剂防治[J]. 吉林农业大学学报， 2013， 35(5): 520-529. (XU W M， XIE Y Y， WANG C W， et al. Chemical control alternaria leaf spot of schisandra caused by Alternaria tenuissima[J]. Journal of Jilin Agricultural University， 2013， 35(5): 520-529.)[4]吉丽， 田磊， 卢宝慧， 等. 大豆灰霉病和黑斑病拮抗菌株的筛选和鉴定[J]. 土壤与作物， 2020， 9(1): 31-39. (JI L， TIAN L， LU B H， et al. Screening and identification of antagonistic strains of soybean Botrytis cinerea and Alternaria tenuissima[J]. Soils and Crops， 2020， 9(1): 31-39.)[5]HARTMAN G L， 王探应， 陈雯. 大豆红叶斑病[J]. 植物检疫， 1989(1): 65-68. (HARTMAN G L， WANG T Y， CHEN W. Red leaf blotch of soybean[J]. Plant Quarantine， 1989(1): 65-68.)[6]常雪艳， 严进. 大豆红叶斑病[J]. 植物检疫， 2009， 23(1): 4. (CHANG X Y， YAN J. Red leaf blotch of soybean[J]. Plant Quarantine， 2009， 23(1): 4.)[7]靳学慧, 吴兴泉, 马汇泉, 等. 大豆壳针孢菌生物学特性的研究[J]. 植物保护学报, 1996(3): 285-286. (JIN X H, WU X Q, MA H Q, et al. Studies on the biological characteristics of Septoria glycines hemmi[J]. Journal of Plant Protection, 1996(3): 285-286.)[8]方中达. 植病研究方法[M]. 北京:中国农业出版社， 1998. (FANG Z D. Research methods of plant diseases[M]. Beijing: China Agriculture Press， 1998.)[9]李华义, 何运转, 张艳杰, 等. 掌叶半夏疫病病原菌的分离与鉴定[J]. 河北农业大学学报, 2016, 39(4): 68-72. (LI H Y, HE Y Z, ZHANG Y J, et al. Isolation and identification of blight disease on Pinellia pedatisecta[J]. Journal of Agricultural University of Hebei, 2016, 39(4): 68-72. [10]丛丽丽， 康俊梅， 张铁军， 等. 苜蓿镰刀菌根腐病病原菌的分离鉴定与致病性分析[J]. 草地学报， 2017， 25(4): 857-865. (CONG L L， KANG J M， ZHANG T J， et al. Identification and pathogenicity test of pathogenic Fusarium of alfafa root rot[J]. Acta Agrestia Sinica， 2017， 25(4): 857-865.)[11]ANALIA E P，〖KG(0.3mm〗 MARA V M. First report of Phoma sorghina (Sacc.) Boerema Dorenbosch & van Kest on wheat leaves (Triticum aestivum L.) in Argentina[J]. Mycopathologia， 2005， 159(1): 75-78.[12]PAOUTOV S. Genetic variation of Phoma sorghina isolates from Southern Africa and Texas[J]. Folia Microbiologica， 2009， 54(3): 217-229.[13]OLIVEIRA R C， DAVENPORT K W， HOVDE B， et al. Draft genome sequence of sorghum grain mold fungus Epicoccum sorghinum， a producer of tenuazonic acid[J]. Genome Announcements， 2017， 5(4): e01495-16.[14]朱香. Epicoccum sorghinum的分离及多基因转玉米株系的抗性鉴定[D]. 武汉: 华中农业大学, 2018. (ZHU X. Isolation of Epicoccum sorghinum and resistance identification of multi-transgenes maize lines[D]. Wuhan: Huazhong Agricultural University, 2018.)[15]张国辉， 李向阳， 顾焕先， 等. 黔东南州红心猕猴桃叶尖干枯病病原鉴定[J]. 中国农学通报， 2021， 37(3): 145-149. (ZHANG G H， LI X Y， GU H X， et al. Identification of pathogens of dry blight disease in leaf tip of red-fleshed kiwi fruit in Qiandongnan prefecture[J]. Chinese Agricultural Science Bulletin， 2021， 37(3):145-149.)[16]ROHIK T， HUDEC K. Fungal infection of malt barley kernels in Slovak Republic[J]. Plant Protection Science， 2007， 43(3): 86-93.[17]LIU L M， ZHAO Y， ZHANG Y L， et al. First report of leaf spot disease on rice caused by Epicoccum sorghinum in China[J]. Plant Disease， 2020， 104(10):2735.[18]DU Y X， XU Y， JIANG J Y， et al. First report of Epicoccum sorghinum causing leaf spot on atractylodes lancea in Hubei Province， China[J]. Plant Disease， 2020， 104(11):3082.[19]黄鈜琳, 尹桥秀, 江仕龙, 等. 茶叶斑病病原菌高粱附球菌的鉴定及生物学特性[J]. 热带作物学报, 2021, 42(11): 3269-3277. (HUANG H L, YIN Q X, JIANG S L, et al. Identification and biological characteristics of Epicoccum sorghinum, pathogen of Tea Leaf Spot[J]. Chinese Journal of Tropical Crops, 2021, 42(11): 3269-3277.)[20]曾慧兰， 卢其能， 曾鹏远， 等. 百合叶尖干枯病病原菌的鉴定， 生物学特性及其对杀菌剂的敏感性研究[J]. 园艺学报， 2018， 45(12): 2407-2416. (ZENG H L， LU Q N， ZENG P Y， et al. Identification， biological characteristics and sensitivity of the causal pathogen inducing leaf dieback on lily[J]. Acta Horticulturae Sinica， 2018， 45(12): 2407-2416.)[21]FU R T， CHEN C， WANG J， et al. First report of Epicoccum sorghinum causing leaf spot on Paris polyphylla in China[J]. Plant Disease， 2019， 103(6): 1426.[22]CHEN X L， WANG Y H， LUO T. First report of leaf spot caused by Phoma sorghina on Oxalis debilis in China[J]. Plant Disease， 2017， 101(6): 1047.[23]YU L， SHE X M， LAN G B， et al. First report of leaf spot caused by Epicoccum sorghinum on Chinese flowering cabbage (Brassica parachinensis) in China[J]. Plant Disease， 2019， 103(11): 2966.[24]彭成彬, 王泽榕, 阮俊峰, 等. 柳叶蜡梅真菌病害分离鉴定及生物学特性[J]. 福建农业学报, 2021, 36(4): 457-463. (PENG C B, WANG Z R, RUAN J F, et al. Identification and biology of leaf spot pathogen on Chimonanthus salicifolius[J]. Fujian Journal of Agricultural Sciences, 2021, 36(4): 457-463.)[25] SAXENA S C. Identification of resistance sources to banded leaf and sheath blight (Rhizoctonia solani f. sp. sasakii) in maize[J]. Indian Phytopathology， 2013， 60(2): 162-166.

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Last Update: 2022-06-30