|Table of Contents|

Cloning and Plant Expression Vector Construction of Drought-Induced GmNF-YA7(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2022年01期
Page:
43-048
Research Field:
Publishing date:

Info

Title:
Cloning and Plant Expression Vector Construction of Drought-Induced GmNF-YA7
Author(s):
YIN Shu-xin1 JI Jun-jie1 XIAO Qing1 LI Ming-yang1 HE Jia-qi1 ZHANG Jun2 ZHAI Ying1
(1.College of Life Science and Agro-Forestry of Qiqihar University/Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Biodiversity Protection in Cold Regions, Qiqihar 161006, China; 2.Branch of Animal Husbandry and Veterinary, Heilongjiang Academy of Agricultural Sciences, Qiqihar 161005, China)
Keywords:
soybean GmNF-YA7 drought response plant expression vector genetically engineered strain
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2022.01.0043
Abstract:
In order to identify the relationship between soybean nuclear factor YA (NF-YA) and abiotic stress, we detected the expression levels of GmNF-YA7 and GmNF-YA8 in soybean under drought stress, cloned the GmNF-YA7, constructed the plant expression vector, and obtained genetically engineered strain in this reaserch. qRT-PCR results showed that both expression levels of GmNF-YA7 and GmNF-YA8 could be induced by drought stress, and the response of GmNF-YA7 to drought stress was more obvious. GmNF-YA7 was cloned from soybean, it was located on chromosome 8 of soybean and encoded a protein containing 336 amino acids. The predicted molecular weight of GmNF-YA7 protein was 37.06 kDa and the pI was 611. The amino acid sequence of GmNF-YA7 protein contained a conserved CBF domain. Subcellular localization prediction results showed that GmNF-YA7 protein was localized in the nucleus. Phylogenetic analysis showed that GmNF-YA7 protein was closely related to Arabidopsis AtNF-YA1 protein. GmNF-YA7 was constructed into plant expression vector pRI101 with restriction endonucleases Nde I and Sal I and transformed into Agrobacterium EHA105. This study showed that the GmNF-YA7 gene was up regulated by drought, and we constructed plant expression vector with GmNF-YA7 and genetically obtained engineered Agrobacterium strain with this vector.

References:

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Last Update: 2022-01-28