|Table of Contents|

Bioinformatics and Expression Analysis of Soybean GmNF-YB2 Gene and Interaction Identification of GmNF-YA10(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2022年01期
Page:
20-027
Research Field:
Publishing date:

Info

Title:
Bioinformatics and Expression Analysis of Soybean GmNF-YB2 Gene and Interaction Identification of GmNF-YA10
Author(s):
ZHAO Guo-qin1WANG Yi2YU Yue-hua2NI Zhi-yong1
(1.College of Life Sciences, Xinjiang Agricultural University, Urumqi 830052, China; 2.College of Agriculture, Xinjiang Agricultural University, Urumqi 830052, China)
Keywords:
soybean GmNF-YB2 bioinformatics tissue drought expression protein interaction
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2022.01.0020
Abstract:
The NF-Y trimer complex is composed of 3 different subunits, NF-YA, NF-YB and NF-YC. In order to explore the interaction between soybean GmNF-YB2 subunits in the NF-Y trimer complex and the response to drought stress, in this study, we cloned GmNF-YB2 gene, performed bioinformatics analysis and expression pattern analysis, and identified the protein interaction relationship between GmNF-YA10 and GmNF-YB2. The results showed that GmNF-YB2 gene was 1 109 bp in length, with a coding region of 498 bp, and encoding 165 amino acids. The molecular weight of the protein was predicted to be 18.19 kD and the theoretical isoelectric point was 6.10. The phylogenetic tree analysis showed that the protein was in the same branch with AtNF-YB2 and AtNF-YB3. Transcriptomic data and tissue expression pattern analysis showed that GmNF-YB2 gene was expressed in all the detected tissues, and the expression level was higher in cotyledons and leaves. The expression of GmNF-YB2 gene was decreased in leaves under drought stress. Yeast two-hybrid analysis showed that there was no protein interaction between GmNF-YA10 and GmNF-YB2.

References:

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Last Update: 2022-01-28