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Genetic Analysis of Resistance to Phytophthora sojae PsJS2 and Mapping of Resistance Gene in Soybean Cultivar Andou 1498(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2021年05期
Page:
628-632
Research Field:
Publishing date:

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Title:
Genetic Analysis of Resistance to Phytophthora sojae PsJS2 and Mapping of Resistance Gene in Soybean Cultivar Andou 1498
Author(s):
CHEN Ya-guangZAN KaiXU Shu-xiaWANG Feng-juZHANG Zhi-minZHENG Li-minZHOU QingSHEN Wei-min
(Anyang Academy of Agricultural Sciences/Henan Province High Quality Soybean Improvement Engineering Technology Research Center,Anyang 455000,China)
Keywords:
Andou 1498Phytophthora root rot(PRR)Molecular markerResistant gene
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2021.05.0628
Abstract:
In order to discover new disease resistance genes to enhance soybean resistance to Phytophthora root rot(PRR).In this study,we used the hypocotyls inoculation technique to identify the resistance of soybean cultivars Andou 1498 and Williams to eight Phytophthora sojae (P.sojae) including Race1,Race3,Race4,NKI,USAR2,Ps41-1,PsMC1 and PsJS2.The F2∶3 generation population derived from the cross of Williams×Andou 1498 were inoculated P.sojae PsJS2 for each generation of the cross of Andou 1498 and Williams for resistance genetic analysis.618 SSR molecular markers were used for preliminary mapping of antagonistic genes.The results showed that Andou 1498 was resistant to six soybean Phytophthora strains,such as Race1,Race3,Race4,Ps41-1,Psmc1 and Psjs2,indicating that Andou 1498 was an excellent material resistant to Phytophthora root rot.The results of resistance genetic analysis showed that both the Andou 1498 and F1 generations were resistant to P.sojae PsJS2,Williams was susceptible,and the F2∶3 generation showed a segregation ratio of 1∶2∶1 (resistance:segregation:susceptible).The resistance of Andou 1498 to P.sojae PsJS2 is controlled by a single dominant gene,temporarily named Rps1498.The gene mapping results showed that Rps1498 is linked to 5 SSR markers on soybean chromosome 3(N linkage group) including Satt152,Satt631,Sat_186,Satt683 and Satt624.Basis of linkage analysis with SSR markers the Rps1498 was located on molecular linkage group N and flanked by Sat_186 and Satt683 with genetic distances 5.1 and 9.3 cM,respectively.

References:

[1]Schmitthenner A F.Problems and progress in control of Phytoph-thora root rot of soybean[J].Plant Disease,1985,69(4):362.[2]Tyler B M.Phytophthora sojae:Root rot pathogen of soybean and model oomycete[J].Molecular Plant Pathology,2007,8(1):1-8.[3]沈崇尧,苏彦纯.中国大豆疫霉病菌的发现及初步研究[J].植物病理学报,1991,21(4):298.(Shen C Y,Su Y C.Discovery and preliminary studies of Phytophthora megasperma on soybean in China[J].Acta Phytopathologica Sinica,1991,21(4):298.)[4]朱振东,王晓鸣,常汝镇,等.黑龙江省大豆疫霉菌生理小种鉴定及大豆种质的抗性评价[J].中国农业科学,2000,33(1):62-67.(Zhu Z D,Wang X M,Chang R Z,et al.Identification of race of Phytophthora sojae and reaction of soybean germplasm resources in Heilongjiang Province[J].Scientia Agricultura Sinica,2000,33(1):62-67.)[5]姚海燕,王晓鸣,武小菲,等.大豆品种早熟18抗疫霉根腐病基因的SSR分子标记[J].植物遗传资源学报,2010,11(2):213-217.(Yao H Y,Wang X M,Wu X F,et al.Molecular mapping of Phytophthora resistance gene in soybean cultivar Zaoshu 18[J].Journal of Plant Genetic Resources,2010,11(2):213-217.)[6]Tyler B M.Molecular basis of recognition between Phytophthora pa-thogens and their hosts [J].Annual Review of Phytopathology,2002,40:137-167.[7]〖JP3〗Zhong C,Sun S,Yao L L,et al.Fine mapping and identification of a novel Phytophthora root rot resistance locus RpsZS18 on chromosome 2 in soybean[J].Frontiers in Plant Science,2018,9:44.[8]陈晓玲,朱振东,王晓鸣,等.大豆品种(系)抗疫霉根腐病基因推导[J].中国农业科学,2008,41(4):1227-1234.(Chen X L,Zhu Z D,Wang X M,et al.Postulation of Phytophthora resistance genes in soybean cultivars or lines[J].Scientia Agricultura Sinica,2008,41(4):1227-1234.)[9]张志民,陈亚光,周青,等.安豆1498-疫霉根腐病抗性新种质[J].中国油料作物学报,2017,39(6):855-860.(Zhang Z M,Chen Y G,Zhou Q,et al.Resistance identification of a new soybean germplasm Andou 1498 to Phytophthora root rot[J].Chinese Journal of Oil Crop Sciences,2017,39(6):855-860.) [10]张雪翠,孙素丽,卢为国,等.河南大豆新品系抗大豆疫霉根腐病基因鉴定[J].作物学报,2021,47(2):93-102.(Zhang X C,Sun S L,Lu W G,et al.Identification of resistance gene against Phytophthora root rot in new soybean lines breeded in Henan Province[J].Acta Agronomica Sinica,2021,47(2):93-102.)[11]Zhang J,Xia C,Wang X,et al.Genetic characterization and fine mapping of the novel Phytophthora resistance gene in a Chinese soybean cultivar[J].Theoretical and Applied Genetics,2013,126:1555-1561.[12]Gordon S G,Martin S,Dorrance A E.Rps8 maps to a resistance gene rich region on soybean molecular linkage group F[J].Crop Science,2006,46:168-173.[13]Sharp P J,Kreis M,Shewry P R,et al.Location of β-amylase sequence in wheat and its relatives[J].Theoretical Appllied Genetics,1988,75:289-290.〖HJ2.05mm〗[14]Michelmore R W,Paran I,Kesseli R V.Identification of markers linked to disease-resistance genes by bulked segregant analysis:A rapid method to detect markers in specific genomic regions by using segregating populations[J].Proceedings of the National Academy of Sciences of the United States of America,1991,88:9828-9832.[15]武晓玲,赵晋铭,王永林,等.南京大豆根腐病病原物的分离及毒性鉴定[J].南京农业大学学报,2009,32(2):61-64.(Wu X L,Zhao J M,Wang Y L,et al.Identification and isolation of soybean root rot in Nanjing[J].Journal of Nanjing Agricultural University,2009,32(2):61-64.)[16]郭娜,胡冠军,赵晋铭,等.一对单显性大豆抗疫霉根腐病基因的遗传分析及定位[J].南京农业大学学报,2015,38(4):532-537.(Guo N,Hu G,Zhao J M,et al.Genetic analysis and mapping of a single dominant Phytophthora sojae resistance gene in soybean[J].Journal of Nanjing Agricultural University,2015,38(4):532-537.)[17]Lin F,Zhao M X,Ping J Q,et al.Molecular mapping of two genes conferring resistance to Phytophthora sojae in a soybean landrace PI 567139B[J].Theoretical and Applied Genetics,2013,126:2177-2185.[18]Weng C.Mapping genes conferring resistance to Phytophthora root rot of soybean,Rps1a and Rps7[J].Hered,2001,92:442-446.[19]Sugimoto T,Yoshida S,Watanabe K,et al.Identification of SSR markers linked to the Phytophthora resistance gene Rps1-d in soybean[J].Plant Breeding,2008,127(127):154-159.[20]张海鹏,郭娜,牛景萍,等.大豆品种郑97196对疫霉根腐病的抗性遗传分析及基因定位[J].大豆科学,2016,35(3):373-379.(Zhang H P,Guo N,Niu J P,et al.Genetic analysis of resistance to Phytophthora sojae and mapping of resistance gene in soybean cultivar Zheng 97196[J].Soybean Science,2016,35(3):373-379.) [21]范爱颖,王晓鸣,方小平,等.大豆品种豫豆25抗疫霉根腐病基因的鉴定[J].作物学报,2009,35(10):1844-1850.(Fan A Y,Wang X M,Fang X P,et al.Molecular identification of Phytophthora resistance gene in soybean cultivar Yudou 25[J].Acta Agronomica Sinica,2009,35(10):1844-1850.)[22]张吉清.大豆对疫病的抗性评价、抗病基因挖掘及候选基因分析[D].北京:中国农业科学院,2013.(The evaluation of the phytophthora resistance in soybean cultivars,mining and analysis of the Rps candidate gene[D].Beijing:Chinese Academy of Agricultural Sciences,2013.)[23]Zhu Z D,Wang H B,Wang X M,et al.Distribution and virulence diversity of Phytophthora sojae in China[J].Agricultural Sciences in China,2004(2):116-123.[24]Lohnes D G,Nickell C D,Schmitthenner A F.Origin of soybean alleles for Phytophthora resistance in China[J].Crop Science,1996,36:1689-1692.[25]朱振东,霍云龙,王晓鸣,等.大豆疫霉根腐病抗源筛选[J].植物遗传资源学报,2006(1):24-30.(Zhu Z D,Huo Y L,Wang X M,et al.Screening for resistance sources to Phytophthora root rot in soybean[J].Journal of Plant Genetic Resources,2006(1):24-30.)

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Last Update: 2021-09-27