|Table of Contents|

GmRACK1 Gene Negatively Regulates Drought-Tolerance in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2021年05期
Page:
592-601
Research Field:
Publishing date:

Info

Title:
GmRACK1 Gene Negatively Regulates Drought-Tolerance in Soybean
Author(s):
LIU Xi-pingWANG Shu-jingZHANG Fu-qiangZHANG DanLI Da-hong
(School of Biological and Food Processing Engineering,Huanghuai University,Zhumadian 463000,China)
Keywords:
SoybeanRACK1TransgenesisGene overexpressionGene silencingDrought resistance
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2021.05.0592
Abstract:
Receptor of activated protein kinase C1 (RACK1) belongs to a subfamily of proteins with a repeated structure of the tryptophan-aspartic acid domain(WD).In order to study the regulation of GmRACK1 in drought stress,we constructed overexpression and silencing two binary expression vectors of GmRACK1 gene and transformed into soybean.The control and transgenic lines were treated with drought and rewatering.The dry weight,survival rate,chlorophyll content,hydrogen peroxide content and antioxidant enzyme activity of the lines were measured.We analyzed the regulation of GmRACK1 gene on drought resistance of soybean.The results showed that,under drought stress,the survival rate and dry weight of GmRACK1 gene silenced lines(RNAi) were significantly higher than those of WT and OE lines.After 10 days drought,the chlorophyll content of RNAi lines was significantly higher than that of WT and OE lines.The color of the staining OE lines stained with DAB and NBT was deeper than that of WT and RNAi lines.After 10 days soil drought stress and then 4 days rewatering,RNAi lines recovered better than WT and OE lines.Under 15% PEG 4000 treatment,the activities of SOD,POD,CAT and ABA content of RNAi strains were significantly higher than those of WT and OE strains,while MDA and conductivity were significantly lower than those of WT and OE strains.These results indicated that RNAi strain could remove excessive reactive oxygen species,which will weaken the oxidative damage to plants,so the GmRACK1 gene could regulate the drought tolerance of soybean.

References:

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Last Update: 2021-09-27