|Table of Contents|

Molecular Evolutionary and Flowering Regulation Function Analysis of TOE Genes in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2021年05期
Page:
581-591
Research Field:
Publishing date:

Info

Title:
Molecular Evolutionary and Flowering Regulation Function Analysis of TOE Genes in Soybean
Author(s):
LIU Jun;  LI Hai-yang;  HE Mi-lan;  ZHANG Ting;  LIU Bao-hui;  ZHAO Xiao-hui
(School of Life Sciences，Guangzhou University，Guangzhou 510006，China)
Keywords:
Soybean;  Flowering regulation;  TOE genes;  Bioinformatics analysis;  GmTOE6b;  Collinearity analysis;  Haplotype
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2021.05.0581
Abstract:
In order to analyze the evolution of GmTOEs and its regulation on flowering function in soybean, to provide the basis for functional analysis of GmTOEs and research on latitude adaptability of soybean, we used bioinformatics methods to perform cluster analysis, sequence characterization analysis, chromosomal segment collinearity analysis and tissue specific expression analysis of GmTOEs, predicted AP2 binding sites in the promoter regions of important flowering genes and verified flowering times in different haplotypes of soybean. The results showed that 12 GmTOEs were retrieved from PlantTFDB database, and GmTOE6b (Glyma.02G087400) was a newly discovered TOE gene in soybean. GmTOE6a and GmTOE6b had only one AP2 domain, while the other GmTOEs had two AP2 domains. Six GmTOEs were clustered into the same group as the Arabidopsis TOE1, two were clustered with Arabidopsis TOE2, four were clustered with Arabidopsis TOE3 and AP2. Each GmTOEs had one miR172 target site, and the target site sequence was highly consistent with AtTOEs. Chromosome segment collinearity analysis showed that GmTOEs could be divided into three categories according to their origin, and six of them were generated with the replication of soybean genome. Four of them originated before the speciation of soybean and shared a common ancestor with AtTOEs. Two of them originated before the speciation of soybean and had no common ancestor with AtTOEs. The important flowering regulation genes GmFT2a and GmFT5a have multiple AP2 binding sites in their promoter, and both GmTOE4b and GmTOE5b can affect the flowering time of soybean. The results indicated that the 12 GmTOEs were the most likely to be the target of miR172. Although the amino acid composition of the protein encoded by them was very similar, their evolutionary rules and tissue-specific expression were different, and they may have functional differentiation during the evolutionary process. GmTOE4b and GmTOE5b may regulate the transcription of GmFT2a and GmFT5a by binding to their cis acting elements on the promoters, to regulate flowering.

References:

［1］Graham P H, Vance C P. Legumes: Importance and constraints to greater use［J］. Plant Physiology, 2003, 131(3): 872-877.

［2］姚林. 中美贸易摩擦下的中国大豆产业现状与发展趋势［J］. 中国油脂, 2020, 45(2): 10-14. (Yao L. Current situation and development trend of China′s soybean industry under Sino-US trade friction［J］. China Oils and Fast, 2020, 45(2): 10-14.)
［3］查霆, 钟宣伯, 周启政, 等. 我国大豆产业发展现状及振兴策略［J］. 大豆科学, 2018, 37(3): 458-463. (Zha T, Zhong X B, Zhou Q Z, et al. Development status of China′s soybean industry and strategies of revitalizing［J］. Soybean Science, 2018, 37(3): 458-463.)
［4］Watanabe S, Harada K, Abe J. Genetic and molecular bases of photoperiod responses of flowering in soybean［J］. Breeding Science, 2012, 61(5): 531-543.
［5］夏正俊. 大豆光周期反应与生育期基因研究进展［J］. 作物学报, 2013, 39(4): 571-579. (Xia Z J. Research progresses on photoperiodic flowering and maturity genes in soybean (Glycine max Merr.)［J］. Acta Agronomica Sinica, 2013, 39(4): 571-579.)
［6］Xia Z J, Watanabe S, Yamada T, et al. Positional cloning and characterization reveal the molecular basis for soybean maturity locus E1 that regulates photoperiodic flowering［J］. Proceedings of the National Academy of Sciences, 2012, 109(32): E2155-E2164.
［7］Watanabe S, Xia Z J, Hideshima R, et al. A map-based cloning strategy employing a residual heterozygous line reveals that the GIGANTEA gene is involved in soybean maturity and flowering［J］. Genetics Society of America, 2011, 188(2): 395-407.
［8］Watanabe S, Hideshima R, Xia Z J, et al. Map-based cloning of the gene associated with the soybean maturity locus E3［J］. Genetics Society of America, 2009, 182(4): 1251-1262.
［9］Liu B H, Kanazawa A, Matsumura H, et al. Genetic redundancy in soybean photoresponses associated with duplication of the phytochrome A gene［J］. Genetics Society of America, 2008, 180(2): 995-1007.
［10］Dissanayaka A, Rodriguez T O, Di S, et al. Quantitative trait locus mapping of soybean maturity gene E5［J］. Breeding Science, 2016, 66(3): 407-415.
［11］Cober E R. Long juvenile soybean flowering responses under very short photoperiods［J］. Crop Science, 2011, 51(1): 140-145.
［12］Cober E R, Voldeng H D. Low R:FR light quality delays flowe-ring of E7E7 soybean lines［J］. Crop Science, 2001, 41(6): 1823-1826.
［13］Cober E R, Molnar S J, Charette M, et al. A new locus for early maturity in soybean［J］. Crop Science, 2010, 50(2): 524-527.
［14］Kong F J, Nan H Y, Cao D, et al. A new dominant gene E9 conditions early flowering and maturity in soybean［J］. Crop Science, 2014, 54(6): 2529-2535.
［15］Samanfar B, Molnar S J, Charette M, et al. Mapping and identification of a potential candidate gene for a novel maturity locus, E10, in soybean［J］. Theoretical and Applied Genetics, 2017, 130(2): 377-390.
［16］Wang F F, Nan H Y, Chen L Y, et al. A new dominant locus, E11, controls early flowering time and maturity in soybean［J］. Molecular Breeding, 2019, 39(5): 70.
［17］Lu S J, Zhao X H, Hu Y L, et al. Natural variation at the soybean J locus improves adaptation to the tropics and enhances yield［J］. Nature Genetics, 2017, 49(5): 773-779.
［18］Schmutz J, Cannon S B, Schlueter J, et al. Genome sequence of the palaeopolyploid soybean［J］. Nature, 2010, 463(7278): 178-183.
［19］Cao D, Li Y, Lu S J, et al. GmCOL1a and GmCOL1b function as flowering repressors in soybean under long - day conditions［J］. Plant and Cell Physiology, 2015, 56(12): 2409-2422.
［20］Takeshima R, Hayashi T, Zhu J, et al. A soybean quantitative trait locus that promotes flowering under long days is identified as FT5a, a FLOWERING LOCUS T ortholog［J］. Journal of Experimental Botany, 2016, 67(17): 5247-5258.
［21］Jiang B J, Zhang S W, Song W W, et al. Natural variations of FT family genes in soybean varieties covering a wide range of maturity groups［J］. BMC Genomics, 2019, 20(1): 230.［22］Sun F, Xu M L, Park C, et al. Characterization and quantitative trait locus mapping of late - flowering from a Thai soybean cultivar introduced into a photoperiod - insensitive genetic background［J］. PLoS One, 2019, 14(12): e0226116.［23］Kong F J, Liu B H, Xia Z J, et al. Two coordinately regulated homologs of FLOWERING LOCUS T are involved in the control of photoperiodic flowering in soybean［J］. Plant Physiology, 2010, 154(3): 1220-1231.［24］Guo G Y, Xu K, Zhang X M, et al. Extensive analysis of GmFTL and GmCOL expression in northern soybean cultivars in field conditions［J］. PLoS One, 2015, 10(9): e0136601.［25］Kim S, Soltis P S, Wall K, et al. Phylogeny and domain evolution in the APETALA2-like gene family［J］. Molecular Biology and Evolution, 2006, 23(1): 107-120.［26］Schmid M, Uhlenhaut N H, Godard F, et al. Dissection of floral induction pathways using global expression analysis［J］. Development, 2003, 130(24): 6001-6012.［27］Aukerman M J, Sakai H. Regulation of flowering time and floral organ identity by a microRNA and its APETALA2 - like target genes［J］. The Plant Cell, 2003, 15(11): 2730-2741.［28］Lee Y S, Lee D Y, Cho L H, et al. Rice miR172 induces flowering by suppressing OsIDS1 and SNB, two AP2 genes that negatively regulate expression of Ehd1 and florigens［J］. Rice, 2014, 7(1), 31.［29］Wu G, Park M Y, Conway S R, et al. The sequential action of miR156 and miR172 regulates developmental timing in Arabidopsis［J］. Cell, 2009, 138(4): 750-759.［30］Martin A, Adam H, Díaz-Mendoza M, et al. Graft - transmissible induction of potato tuberization by the microRNA miRl72［J］. Development, 2009, 136(17): 2873-2881.［31］Zhao X H, Cao D, Huang Z J, et al. Dual functions of GmTOE4a in the regulation of photoperiod - mediated flowering and plant morphology in soybean［J］. Plant Molecular Biology, 2015, 88(4): 343-355.［32］Wang T, Sun M Y, Wang X S, et al. Over-expression of GmGIa - regulated soybean miR172a confers early flowering in transgenic Arabidopsis thaliana［J］. International Journal of Molecular Sciences, 2016, 17(5): 645.［33］Kim K D, EI Baidouri M, Abernathy B, et al. A comparative epigenomic analysis of polyploidy - derived genes in soybean and common bean［J］. Plant Physiology, 2015, 168(4): 1433-1447.［34］Lu S, Dong L D, Kong F J, et al. Stepwise selection on home-ologous PRR genes controlling flowering and maturity during soybean domestication［J］. Nature Genetics, 2020, 52(4): 428-436.［35］Chen C J, Chen H, Zhang Y, et al. TBtools: An integrative toolkit developed for interactive analyses of big biological data［J］. Molecular Plant, 2020, 13(8): 1194-1202.［36］Jofuku K D, den Boer B G, van Montagu M, et al. Control of Arabidopsis flower and seed development by the homeotic gene APETALA2［J］. The Plant Cell, 1994, 6(9): 1211-1225.［37］Castillejo C, Pelaz S. The balance between CONSTANS and TEMPRANILLO activities determines FT expression to trigger flowering［J］. Current Biology, 2008, 18(17): 1338-1343.

Memo

Memo:
-
Last Update: 2021-09-27