|Table of Contents|

Improvement Effects of Soybean Isoflavones on Lipopolysaccharide-Induced Depression-Like Behavior in Mice(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2021年04期
Page:
539-545
Research Field:
Publishing date:

Info

Title:
Improvement Effects of Soybean Isoflavones on Lipopolysaccharide-Induced Depression-Like Behavior in Mice
Author(s):
GAO Rong-jing1; 2;  HUANG Hong3;  JIANG Ning3;  LIU Xin-min3;  SUN Jing2;  LU Cong2;  WANG Feng-zhong1; 2
（1.College of Food Science Technology, Nanjing Agricultural University, Nanjing 210095, China; 2. Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China; 3. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China）
Keywords:
Soybean isoflavones;  Lipopolysaccharide;  Anti-depression;  Inflammatory reaction;  Tryptophan metabolism
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2021.04.0539
Abstract:
The current study was conducted to explore the effects of soybean isoflavones (SI) on lipopolysaccharide (LPS)-induced depression-like behavior in mice and investigate its possible mechanisms. Sixty SPF C57BL/6J male mice were randomly divided into control group, LPS group, SI low dose group(10 mg·kg-1), SI medium dose group(20 mg·kg-1), SI high dose group(40 mg·kg-1) and positive drug (amitriptyline, 10 mg·kg-1) group, with 10 mice in each group. The depression model was established by acute intraperitoneal injection of LPS(1 mg·kg-1) once after treatment with SI for two weeks. The open field test was used to observe spontaneous activity of mice. Sucrose preference test and tail suspension test were performed to evaluate the depression behaviors of mice. The inflammatory factors (interleukin-6, interleukin-1β, and tumour necrosis factor-α), tryptophan metabolites kynurenine(KYN) and serotonin(5-HT) in the cortex of mice were measured by ELISA. The results of behavioral tests showed that SI did not affect the spontaneous activities of mice, but reversed the decrease of sucrose preference index and prolonged immobility of tail suspension induced by LPS. The results of ELISA showed that compared with control group, LPS treatment significantly increased the contents of pro-inflammatory cytokines and interrupted tryptophan metabolic pathway. However, SI administration effectively reserved these changes. The current study demonstrated that SI had the antidepressant effect, which may be related to inhibit the inflammatory responses and modulate the tryptophan metabolism.

References:

［1］Martins J, Brijesh S. Phytochemistry and pharmacology of anti-depressant medicinal plants: A review［J］. Biomedicine & Pharmacotherapy,2018, 104: 343-365. ［2］Tran B X, Ha G H, Nguyen D N, et al. Global mapping of 〖JP4〗interventions to improve quality of life of patients with depression during 1990-2018［J］.Quality of Life Research, 2020, 29(9): 2333-2343. ［3］何海然，薛占霞. 抑郁症相关发病机制的研究进展［J］. 神经药理学报,2016, 6(2): 20-25. （He H R, Xue Z X. Research progress of depression-related pathogenesis ［J］. Journal of Neuropharmacology, 2016,6(2):20-25.） ［4］李硕，王建. 大豆异黄酮临床应用的研究进展［J］. 大豆科学,2020, 39(4): 633-640. (Li S, Wang J. Research progress in clinical application of soybean isoflavones ［J］. Soybean Science, 2020,39(4):633-640.) ［5］张珍，韩领，夏晓洋，等. 大豆异黄酮神经保护作用的研究进展［J］. 中国食物与营养. 2019, 25(1): 53-57.（Zhang Z, Han L, Xia X Y, et al. Research progress on neuroprotection of soybean isoflavones ［J］. Chinese Food and Nutrition, 2019,25(1):53-57.） ［6］Gleason C E, Fischer B L, Dowling N M, et al. Cognitive effects of soy isoflavones in patients with Alzheimer′s disease［J］. Journal of Alzheimers Disease, 2015, 47(4): 1009-1019. ［7］Jiang T, Wang X, Ding C, et al. Genistein attenuates isoflurane-induced neurotoxicity and improves impaired spatial learning and memory by regulating cAMP/CREB and BDNF-TrkB-PI3K/Akt signaling［J］. The Korean Journal of Physiology & Pharmacology, 2017, 21(6): 579. ［8］王艳，汪远金，蔡标，等. 大豆异黄酮对AD大鼠海马CaM-CaMPK信号转导通路相关蛋白的影响［J］. 中国老年学杂志, 2014, 34(4): 952-954.（Wang Y, Wang Y J, Cai B, et al. Effect of soybean isoflavones on proteins related to CaM-CaMPK signal transduction pathway in hippocampus of AD rats ［J］. Chinese Journal of Gerontology, 2014,34(4):952-954.） ［9］蔡标，彭代银，汪远金，等. 大豆异黄酮对AD大鼠海马NO-cGMP信号转导系统的影响［J］. 中国中药杂志, 2011, 36(2): 220-223. (Cai B, Peng D Y, Wang Y J, et al. Effects of soybean isoflavones on NO-cGMP signal transduction system in hippocampus of AD rats ［J］. Chinese Journal of Traditional Chinese Medicine, 2011,36(2):220-223.)［10］Lu C, Gao R, Lyu J, et al. Neuroprotective effects of soy isoflavones on chronic ethanol-induced dementia in male ICR mice［J］. Food Function, 2020, 11(11): 10011-10021.［11］Lu C, Wang Y, Wang D, et al. Neuroprotective effects of soy isoflavones on scopolamine-induced amnesia in mice［J］. Nutrients, 2018, 10(7):853.［12］何凤琴，向全丽，屈庚超，等. 大豆异黄酮影响C57BL/6J母鼠行为与雌激素受体α的关系［J］. 西安文理学院学报(自然科学版), 2020, 23(1): 50-55. (He F Q, Xiang Q L, Qu G C, et al. The relationship between soybean isoflavones and estrogen receptor α in C57BL/6J female mice ［J］. Journal of Xi′an University of Arts and Sciences (Natural Science Edition), 2020,23(1):50-55.)［13］黄蕊，罗涛，刘永芳，等. 丙泊酚对脂多糖诱导的脓毒血症小鼠抑郁样行为和海马炎症反应的影响［J］. 神经损伤与功能重建. 2020, 15(6): 325-328. (Huang R, Luo T, Liu Y F, et al. Effects of propofol on depressive behavior and hippocampal inflammatory response in lipopolysaccharide-induced sepsis mice ［J］.Nerve Injury and Functional Reconstruction, 2020,15(6):325-328.)［14］薄秀梅，贺玲，张荣丽，等. 姜黄素对脂多糖诱导的小鼠抑郁样行为的影响及机制［J］. 安徽医科大学学报, 2020, 55(7): 1064-1068. (Bo X M, He L, Zhang R L, et al. Effect of curcumin on lipopolysaccharide-induced depression-like behavior in mice and its mechanism ［J］. Journal of Anhui Medical University, 2020,55(7):1064-1068.)［15］袁璐，李晋熙，罗跃嘉. 脂多糖连续注射诱发小鼠抑郁行为及认知改变［J］. 成都医学院学报, 2017, 12(5): 536-540.(Yuan L, Li J X, Luo Y J. Depressive behavior and cognitive changes induced by continuous injection of lipopolysaccharide in mice ［J］. Journal of Chengdu Medical College, 2017, 12(5): 536-540.)［16］黄红，陈碧清，姜宁，等. 鲜天麻对睡眠干扰诱导小鼠学习记忆障碍的改善作用［J］. 中草药, 2020, 51(9): 2509-2516. (Huang H, Chen B Q, Jiang N, et al. Effect of fresh Gastrodiaelata Blume on learning and memory impairment induced by sleep disturbance in mice ［J］. Chinese Herbal Medicine, 2020,51(9):2509-2516.)［17］Strekalova T, Spanagel R, Bartsch D, et al. Stress-induced anhedonia in mice is associated with deficits in forced swimming and exploration［J］. Neuropsychopharmacology, 2004, 29(11): 2007-2017.［18］Jiang N, Wang H, Li C, et al. The antidepressant-like effects of the water extract of Panax ginseng and Polygala tenuifolia are mediated via the BDNF-TrkB signaling pathway and neurogenesis in the hippocampus［J］. Journal of Ethnopharmacology, 2021, 267: 113625.［19］冯殿伟. 抑郁症治疗靶标及其药物研发进展［J］. 中国医院药学杂志, 2018, 38(4): 443-449. (Feng D W. Progress in research and development of therapeutic targets and drugs for depression ［J］. Chinese Journal of Hospital Pharmacy, 2018,38(4):443-449.)［20］Remus J L, Dantzer R.Inflammation models of depression in rodents: Relevance to psychotropic drug discovery［J］. The International Journal of Neuropsychopharmacology, 2016, 19(9): pyw028.［21］Lichtblau N, Schmidt F M, Schumann R, et al. Cytokines as biomarkers in depressive disorder: Current standing and prospects［J］. International Review of Psychiatry,2013, 25(5): 592-603.［22］胡明会，李亚琼，张浩，等. 基于抑郁症动物模型炎症机制的研究进展［J］. 医学综述, 2019, 25(12): 2294-2298. ( Hu M H, Li Y Q, Zhang H, et al. Research progress of inflammation mechanism based on depression animal model ［J］. Medical Review, 2019,25(12):2294-2298.)［23］Bekris S, Antoniou K, Daskas S, et al. Behavioural and neurochemical effects induced by chronic mild stress applied to two different rat strains［J］. Behavioural Brain Research, 2005, 161(1): 45-59.［24］Lim D W, Park J, Han D, et al. Anti-inflammatory effects of Asianfawn Lily (Erythronium japonicum) extract on lipopolysaccharide-induced depressive-like behavior in mice［J］. Nutrients, 2020, 12(12): 3809.［25］Zhang L, Zhao N, Guo W, et al. Antidepressant-like and anxiolytic-like effects of YL-IPA08, a potent ligand for the translocator protein (18 kDa)［J］. Neuropharmacology,2014, 81: 116-125.［26］Valkanova V, Ebmeier K P, Allan C L. CRP, IL-6 and depression:A systematic review and meta-analysis of longitudinal studies［J］. Journal of Affective Disorders, 2013, 150(3): 736-744.［27］Schmidt F M, Lichtblau N, Minkwitz J, et al. Cytokine levels in depressed and non-depressed subjects, and masking effects of obesity［J］. Journal of Psychiatric Research,2014, 55: 29-34.［28］Wang J, Jia Y, Li G, et al. The dopamine receptor D3 regulates lipopolysaccharide-induced depressive-like behavior in mice［J］. The International Journal of Neuropsychopharmacology, 2018, 21(5): 448-460.［29］吴月容，谷有全，秦红岩. 色氨酸代谢对卒中后抑郁影响的研究［J］. 临床神经病学杂志, 2020, 33(3): 222-225. (Wu Y R, Gu Y Q, Qin H Y. The effect of tryptophan metabolism on post-stroke depression ［J］. Journal of Clinical Neurology, 2020,33(3):222-225.)［30］O′Connor J C, Andre C, Wang Y, et al. Interferon-gamma and tumor necrosis factor-alpha mediate the upregulation of indoleamine 2,3-dioxygenase and the induction of depressive-like behavior in mice in response to bacillus Calmette-Guerin［J］. Journal of Neuroscience,2009, 29(13): 4200-4209.［31］Walker A K, Budac D P, Bisulco S, et al. NMDA receptor blockade by ketamine abrogates lipopolysaccharide-induced depressive-like behavior in C57BL/6J mice［J］. Neuropsychopharmacology, 2013, 38(9): 1609-1616.［32］屈悦. 抗抑郁药物的临床应用进展［J］. 蛇志, 2010, 22(2): 142-144. (Qu Y. Clinical application progress of antidepressants ［J］. Journal of Snake, 2010,22(2):142-144.)［33］陈景清，张丽，付凤珍，等. 帕罗西汀、文拉法辛、阿米替林对首发抑郁症认知功能的影响［J］. 精神医学杂志, 2011, 24(4): 263-266. (Chen J Q, Zhang L, Fu F Z, et al. Effects of paroxetine, venlafaxine and amitriptyline on cognitive function of first-episode depression ［J］. Journal of Psychiatry, 2011,24(4):263-266.)

Memo

Memo:
-
Last Update: 2021-08-06