|Table of Contents|

Mapping of Distichous Alternate Phyllotaxis Gene in Cultivated Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2021年04期
Page:
457-465
Research Field:
Publishing date:

Info

Title:
Mapping of Distichous Alternate Phyllotaxis Gene in Cultivated Soybean
Author(s):
(XU Wei-jia1;  LU Jin1;  GAO Hui-hui1;  WAN Ming-yue1;  LI Jia-jia1;  MIAO Long1;  WANG Xiao-bo1;  QIU Li-juan2
（1.College of Agriculture, Anhui Agricultural University,Hefei 230036, China; 2.Institute of Crop Science, Chinese Academy of Agricultural Sciences/The National Key Facility for Crop Gene Resources and Genetic Improvement(NFCRI)/Key Laboratory of Crop Gene Resource and Germplasm Enhancement (MOA), Beijing 100081, China）
Keywords:
Soybean;  Plant architecture;  Distichous alternate phyllotaxis;  BSA-Seq;  Mutant;  Cytokinin
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2021.04.0457
Abstract:
The distichous alternate phyllotaxis was a plane arrangement of all three compound leaves. In order to provide a reference for the formation mechanism of distichous alternate phyllotaxis in cultivated soybean (Glycine max), and promote the plant type research and molecular genetic improvement of soybean under dense condition, this study investigated a distichous phyllotaxis novel germplasm (Wanzhonghuang 601) obtained by EMS mutagenesis of soybean cultivar Zhongpin 661. We used this mutant in combination with the Zhonghuang 13 configuration, and investigated the type of F5 plants. And then we constructed the mixed pools by using F5 decussate alternate and distichous alternate traits, respectively, and performed the gene mapping by BSA-Seq method, and took on the GO functional annotation analysis. The results showed that, the average comparison efficiency between the BSA-Seq and the reference genome was 94.30%, and the average coverage depth was 38.01×. According to the SNP-index and Indel-index association analysis, four regions were mapped on chromosomes 14 and 15, comprising a total of 216 genes. Through GO analysis, there were five genes responding to cytokinin, four genes responding to ethylene, one gene responding to gibberellin, and eight genes responding to auxin. Meanwhile, the content of CTK in SAM (shoot apical meristem) and leaf segment of distichous phyllotaxis soybean were significantly lower than that of alternate phyllotaxis soybean, as well as the CTK content of SAM in distichous phyllotaxis soybean was lower than leaf segment, however, it was opposite in alternate phyllotaxis soybean. These results indicated that the difference of cytokinin distribution may be an important reason for the formation of distichous alternate phyllotaxis.

References:

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Last Update: 2021-08-06