|Table of Contents|

Study on Residue of Metribuzin in Summer Soybean Based on QuEChERS-HPLC-MS(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2021年03期
Page:
403-411
Research Field:
Publishing date:

Info

Title:
Study on Residue of Metribuzin in Summer Soybean Based on QuEChERS-HPLC-MS
Author(s):
ZHANG Le1;  ZHAO Wen-wen2;  LIAO Jin-lin3;  GUO Jing-yuan1;  SHAO Hua1;  SHE Yong-xin1;  JIN Fen1;  WANG Jing1
(1.Institute of Quality Standard and Testing Technology for Agro-products, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2.College of Forestry, Hainan University, Haikou 570228, China; 3.The Central Institute of Socialism, Beijing 100081, China)
Keywords:
Metribuzin;  Summer soybean;  QuEChERS;  HPLC-MS;  Stability
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2021.03.0403
Abstract:
In order to effectively analyze the residue safety of metribuzin in summer soybean(with pod) , green bean straw, soybean and soybean straw, this study established a QuEChERS sample pretreatment coupled with High Performance Liquid Chromatography-tandem Mass Spectrometry (HPLC-MS), to evaluate the residual safety of 70% metribuzin water dispersible granule in summer soybeans sampled from six areas in one year. The extraction of metribuzin from summer soybean green beans(with pod), green bean straw, soybean and soybean straw were extracted by QuEChERS pretreatment technology with acetonitrile, purified by PSA and C18 simultaneously, then scanned by MS spectrometry multi-reaction monitoring mode, and quantified by matrix matching standard curve external standard method. Metribuzin in 0.01-0.20 mg?L-1 range has a good linear relationship, the correlation coefficient was 0.999 7, the standard addition concentrations were 0.01, 0.05 and 0.20 mg?kg-1 , the average recovery of metribuzin in green bean (with pod), green beans straw, soybean and soybean straw was 96.3%-101.7%, the relation standard deviation (RSD) was 1.1%-5.2%, and the quantitative limit was 0.01 mg?kg-1. This method is simple, rapid, sensitive and accurate, meeting to the requirement of detecting the residual quantity of metribuzin in summer soybean. The final residues of 70% metribuzin water dispersible granule in green beans(with pod), green beans straw, soybean and soybean straw were all less than 0.010 mg?kg-1, which were lower than the MRL value of 0.05 mg?kg-1 metribuzin in soybean stipulated by China.

References:

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Last Update: 2021-07-20