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¡¶´ó¶¹¿Æѧ¡·[ISSN:1000-9841/CN:23-1227/S]

Issue:

2021Äê03ÆÚ

Page:

319-326

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Title:

Selection and Bioinformatics Analysis of bHLH Transcription Factor Response to Submergence Stress in Soybean

Author(s):

LIN Yan-hui1;  XU Ran2;  ZHU Hong-lin1;  TANG Li-qiong1;  HOU Ben-jun1;  XU Jing1

(1.Institute of Food Crops, Hainan Academy of Agricultural Sciences/Hainan Key Laboratory of Crop Genetics and Breeding/Hainan Scientific Research Station of Crop Gene Resource & Germplasm Enhancement, Ministry of Agriculture, Haikou 571100, China; 2.Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China)

Keywords:

Soybean;  Submergence tolerance;  Transcriptome;  bHLH transcription factor;  GmbHLH25-15;  Ammonium transporter

PACS:

-

DOI:

10.11861/j.issn.1000-9841.2021.03.0319

Abstract:

In order to study the correlation between bHLH transcription factors and waterlogging responses of soybean, and to provide theoretical basis for the study of waterlogging tolerance of soybean, in this study, Qihuang 34, a soybean variety with strong submergence resistance, was treated with submergence stress. We analyzed the transcriptome sequencing results, selected and took bioinformatics analysis on the differentially expressed bHLH transcription factors. We used fluorescence quantitative PCR to verify the expression of bHLH transcription factor genes, and analyzed the structure and interacting proteins of the gene. The results showed that seven differentially expression bHLH transcription factors were selected, the domain of these protein sequences were not highly homologous, and they belonged to different types of bHLH combining with the transcriptome data. GmbHLH25-15£¨Glyma.15G06680£© showed the most significant expression changes and the quantitative PCR analysis result was consistent with the trend of transcriptome data, showing down-regulated expression. Furthermore, all the 10 proteins interacting with Glyma.15G06680 were ammonium transporters. Among them, the coding genes of 4 proteins showed significantly different expression in transcriptome sequencing results. It was speculated that soybean mainly absorbed ammonium nitrogen as its own nutrient supply in the absence of oxygen, and the bHLH transcription factor GmbHLH25-15 might regulate the absorption of ammonium nitrogen by regulating the ammonium transporter, further resist submergence stress.

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Last Update: 2021-07-20