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¡¶´ó¶¹¿Æѧ¡·[ISSN:1000-9841/CN:23-1227/S]

Issue:

2021Äê03ÆÚ

Page:

289-298

Research Field:

Publishing date:

Info

Title:

Transcriptome Analysis of Soybean Root System in the Early Stage of Rhizobium Infection

Author(s):

QI Ping1;  ZHENG Kai-jie2;  ZHAO Xiao-yu3;  SONG Jian1;  WANG Jun1;  QIU Li-juan4;  YAN Zhe4

(1.College of Agriculture, Yangtze University, Jingzhou 434025, China; 2.Northeast Institute of Geography and Agroecology, CAS, Changchun 130102, China; 3.Institute of Microbiology, Heilongjiang Academy of Sciences, Harbin 150010,China; 4.Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture, Beijing 100081, China)

Keywords:

Soybean;  RNA-seq;  Symbiotic nitrogen fixation;  WIP gene

PACS:

-

DOI:

10.11861/j.issn.1000-9841.2021.03.0289

Abstract:

To analyze signal regulation between rhizobia and host plant in symbiosis, understand gene regulation network of the root system in the early stage of rhizobial infection, and explore the molecular mechanism of evolution of legume symbiosis, the transcriptomic profiles were performed from soybean root tissues infected by rhizobia for 12 h. The significantly regulated genes were analyzed by gene ontology (GO) and KEGG pathway enrichment analysis, the expression of candidate genes in the pathway were verified by qRT-PCR, and evolutionary and functional features of candidate genes were analyzed by phylogenetic tree and correlation cluster analysis. In this research, a total of 488 genes were identified which were significantly regulated by rhizobial infection in the early stage of symbiosis. GO enrichment analysis showed that the up-regulated genes were enriched in wounding response, while the down-regulated genes were enriched in regulation of cell wall biosynthesis. KEGG pathway enrichment analysis showed that the genes involved in plant-pathogen interaction, plant hormone transduction and MAPK signaling pathway were enriched in up-regulated genes, while the genes encoding ABC transporter and circadian rhythm were enriched in down-regulated genes. The analysis of genes enriched in wounding response identified the WIP (Wound-Induced Peptide) family that may be involved in regulation of rhizobia infection in soybeans. RNA-seq and qRT-PCR results showed that 16 WIP genes were induced after rhizobial infection. Molecular evolutionary analysis and gene expression cluster analysis showed that the sequence and expression feature of WIP subfamilies which induced by rhizobia were different from WIP subfamily homologous to ª«Arabidopsis thalianaª«.

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Last Update: 2021-07-20