|Table of Contents|

Dual Priming Oligonucleotide System for Duplex Detection of Southern Bean Mosaic Virus and Tobacco Ring Spot Virus(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2021年02期
Page:
265-269
Research Field:
Publishing date:

Info

Title:
Dual Priming Oligonucleotide System for Duplex Detection of Southern Bean Mosaic Virus and Tobacco Ring Spot Virus
Author(s):
WEI Shuang1 YUAN Jun-jie2 YANG Zhuo-yu2 MA Xin-hua2 LU Nai-hui2 CHEN Wen3WEI Ting-yu4 LONG Yang2
(1.Guangzhou Customs Technology Center, Guangzhou 510000, China; 2.Zhanjiang Customs Technology Center, Zhanjiang 524000, China; 3.Zhanjiang Customs, Zhanjiang 524000, China; 4.Xining Customs Technology Center, Xining 810000, China)
Keywords:
Southern bean mosaic virus Tobacco ring spot virus Imported soybean Seed-borne virus Detection DPO-RT-PCR
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2021.02.0265
Abstract:
Abstract: Southern bean mosaic virus (SBMV) and tobacco ring spot virus (TRSV) are quarantine pests in China, belonging to soybean seed-borne diseases. In order to improve the detection rate of these two viruses in imported soybeans, we designed two pairs of DPO (dual priming oligonucleotide) primers based on specific sequence of SBMV and TRSV in this research, and developed a duplex DPO-RT-PCR method for the detection of SBMV and TRSV. The method has been evaluated for its specificity, sensitivity and annealing temperature sensitivity. The result showed that this method could accurately identify SBMV and TRSV from 10 kinds of plant viruses, showing good specificity and detection sensitivity up to 0.02 ng?L-1. The detection results of this method had no significant difference at the annealing temperature of 45-65 ℃, showing the insensitivity characteristics to annealing temperature. The study provides a new method for high-throughput detection of plant pathogens.

References:

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Last Update: 2021-07-20