|Table of Contents|

Isolation, Identification and Growth Promoting Effect of an Endophytic Bacteria YDX14 in Wild Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2021年02期
Page:
224-231
Research Field:
Publishing date:

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Title:
Isolation, Identification and Growth Promoting Effect of an Endophytic Bacteria YDX14 in Wild Soybean
Author(s):
ZHAO Xiao-yan1;  CAO Yue 1;  DONG Rui-meng1;  ZHU Meng-zhuo1;  WANG Ya-nan1;  ZHU Miao1;  YU Cui-mei2;  MA Lian-ju1
（1.College of Life Science, Shenyang Normal University, Shenyang 110034, China； 2.College of Agronomy, Shenyang Agricultural University, Shenyang 110161, China)
Keywords:
Glycine soja;  Endophytic bacteria;  Identification;  Growth-promoting ability
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2021.02.0224
Abstract:
Abstract: by morphological observation and molecular biological analysis. The growth-promoting characteristics of the strain were studied by measuring the ability to dissolve phosphorus, producing IAA, and producing ACC deaminase. The growth-promoting effect of strain on wheat seedlings was determined by hydroponic growth-promoting test, and the optimum fermentation conditions of strain were determined by a single factor experiment and orthogonal experiment. The results showed that YDX14 colony was white and round, with a sticky and shiny surface, neat edge and rod shape, and its size was (2.0 - 3.2) μm × (0.9 - 1.2) μm. It was a gram-negative bacterium with spores, capsules, and flagella, belonging to Providencia. The strain had the ability to dissolve phosphorus, and the amount of dissolving phosphorus was 46 mg?L-1. The IAA production capacity reached 19.97 mg?L-1. The ACC enzyme activity reached 8.59 μmol?mg-1?h-1. After 10 days of wheat treatment, the growth-promoting effect was the most significant, and the plant height, root length, leaf and root dry weight increased by 58.46%, 81.89%, 54.87% and 29.9% respectively. The contents of soluble sugar and soluble protein were higher than those of control. The optimum fermentation conditions were based on beef paste peptone medium, glucose 1%, yeast extract 1%, calcium chloride 2%, potassium dihydrogen phosphate 0.1%, and pH5.0.

References:

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Last Update: 2021-07-20