|Table of Contents|

Abiotic Stress Induced Expression and Promoter Prediction of GmDof4 and GmDof11 Gene in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2021年02期
Page:
195-220
Research Field:
Publishing date:

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Title:
Abiotic Stress Induced Expression and Promoter Prediction of GmDof4 and GmDof11 Gene in Soybean
Author(s):
ZHANG Jun1 YIN Jun-yi1 HE Jia-qi2 TIAN Qiu-feng1 LIU Qiu-jin1 WANG Yan1 SHI Tong-rui1 ZHAI Ying2
(1.Branch of Animal Husbandry and Veterinary of Heilongjiang Academy of Agricultural Sciences, Qiqihar 161005, China; 2.College of Life Science and Agroforestry, Qiqihar University/Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas, Qiqihar 161006, China)
Keywords:
Soybean Dof transcription factor Abiotic stress Promoter cis-element
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2021.02.0195
Abstract:
Dof family is a typical transcription factors family of plant-specific zinc finger that can respond to abiotic stress in plants. In order to preliminarily study the role and regulation principle of transcription factor coding gene GmDof4 and GmDof11, we studied the major transcription factors of soybean Dof family in abiotic stress resistance. We detected the expressions of GmDof4 and GmDof11 in soybean seedlings under abiotic stress by real-time fluorescence quantitative PCR, and analyzed the cis-elements in the promoters of the two genes with PlantCARE online database. The result showed that the expression of GmDof4 increased under drought, high salinity, high temperature and low temperature stresses. The expression of GmDof11 increased under drought, high salinity and low temperature stresses, but decreased under high temperature stress. The prediction result of promoter cis-elements showed that the GmDof4 promoter contained one anaerobic induction element (ARE), one low temperature responsive element and one MYB transcription factor binding site. The GmDof11 promoter contained three AREs, two LTRs, one defense and stress responsive element (TC-rich repeats) and one MYB transcription factor binding site. In addition, their promoter sequences also contained abscisic acid responsive elements, MeJA responsive elements, gibberellin responsive elements and auxin responsive elements. These results indicated that the promoter regions of GmDof4 and GmDof11 contained stress-related cis-elements, and GmDof4 and GmDof11 were involved in response to abiotic stress in soybean.Keywords:

References:

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Last Update: 2021-07-20