Bioinformatics and Expression Analysis of GmNAC131 Gene in Soybean(PDF)
《大豆科学》[ISSN:1000-9841/CN:23-1227/S]
- Issue:
- 2021年02期
- Page:
- 186-194
- Research Field:
- Publishing date:
Info
- Title:
- Bioinformatics and Expression Analysis of GmNAC131 Gene in Soybean
- Author(s):
- WAN Hui-na; YU Yue-hua; WANG Yi; NI Zhi-yong
- (College of Agriculture, Xinjiang Agricultural University, Urumqi 830052, China)
- Keywords:
- Soybean; GmNAC131 gene; NAC transcription factor; Abiotic stress
- PACS:
- -
- DOI:
- 10.11861/j.issn.1000-9841.2021.02.0186
- Abstract:
- NAC genes have the resistant function to the biological and abiotic stress in many crops. In order to explore the function in soybean, this study cloned a NAC gene GmNAC131 from Williams 82 and analyzed the characteristics of the gene and its coding protein sequence, the protein structure and function, and the expression level of the gene in different tissues by bioinformatics. We analyzed the induced expression of abiotic stress at different time periods by fluorescence quantitative. The cDNA length of GmNAC131 was 1 945 bp, and the open reading frame (ORF) was 1 053 bp. GmNAC131 encoded 350 amino acids, with a molecular weight of 39.49 ku and an isoelectric point of 7.62, contained 3 exons and 2 introns. GmNAC131 contained a highly conserved NAC domain at amino acids from 16 to 166. There was no signal peptide, no transmembrane structure, and the protein composition was hydrophobic. GmNAC131 was located in the nucleus with 8 glycosylation sites and 34 phosphorylation sites. GmNAC131 had a high similarity with NAC proteins of Glycine soja, Vigna unguiculata, Mucuna pruriens, and Cajanus cajan, and shared the same branch with GsNAC. Transcriptome data showed that GmNAC131 gene was expressed in different tissues of soybean, with the highest expression in root and the lowest expression in leaf. The expression of GmNAC131 gene fluctuated during 24 h of low temperature stress at 4 ℃, the expression peaked at 12 h under 250 mmol?L-1 NACL stress, reached its maximum at 0.5 h under 30% PEG6000 stress and reached its maximum at 6 h under 100 mol?L-1 ABA stress.It suggested that the gene might be involved in abiotic stresses such as salt tolerance and drought tolerance in soybean.
Last Update: 2021-07-20