|Table of Contents|

Molecular Marker Screening and Resistance Identification of Soybean Germplasem to SMV Strain SC3

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2021年01期
Page:
1-10
Research Field:
Publishing date:

Info

Title:
Molecular Marker Screening and Resistance Identification of Soybean Germplasem to SMV Strain SC3
Author(s):
CAI Han;  ZHAO Lin;  SHEN Ying;  CHEN Yuan-yuan;  ZHI Hai-jian;  LI Kai
(1.Soybean Research Institute of Nanjing Agricultural University/National Center for Soybean Improvement/Key Laboratory of Biology and Genetic Improvement of Soybean, Ministry of Agriculture/State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing 210095, China;2.Institute of Crops and Ecology, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China)
Keywords:
Soybean mosaic virus;  Molecular markers;  SSR;  CH0211;  Resistance identification;  Coincidence rate of disease resistance phenotypic
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2021.01.0001
Abstract:
To further search for molecular markers closely linked to the resistance genes (Rsc3) of soybean mosaic virus (SMV) prevalent strain SC3, providing an accurate and rapid method for breeding resistant cultivars and promoting the SMV resistance genes mining. In the present study, 30 SSR markers newly designed near the SMV resistance site and 50 SSR markers known to be linked to Rsc3 were preliminary screened via 96 soybean cultivars(lines), and the resistance of288 newly bred soybean varieties(lines) from 2016 to 2019 in China were identified combining with genotype, phenotype and serological identification. The result showed that, one newly developed SSR marker CH0211 was initially screened, and the coincidence rate with the resistance phenotypic selection of soybean varieties was 87.8%. Then, 288 soybean cultivars(lines) were further detected and analyzed by using this marker. With the combination of artificial inoculation phenotype and serological identification, the coincidence rate of disease resistance phenotype and genotype was 80.56%. In this study, 158 soybean cultivars with disease resistance to SC3 were identified from 288 soybean cultivars(lines), accounting for 54.86% of the total tested materials, and the molecular bands of 144 soybean cultivars(lines) were identified that were consistent with the type of disease-resistant cultivars(lines). Totally ， the phenotypes of 116 cultivars(lines) were consistent with the resistance molecular bands, including PI96983, Qihuang 34, Fendou 104, and Shanning 28. All in all, we developed and identified a molecular marker CH0211 closely linked to Rsc3. This marker can be effectively used in germplasm selection of soybean cultivars resistant to SMV SC3 and accelerate the breeding process of soybean resistance to SMV.

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Last Update: 2021-02-09