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Analysis of Soybean GmPM31 Bioinformatics,Tissue Expression and Response to High Temperature and High Humidity(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2021年05期
Page:
612-619
Research Field:
Publishing date:

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Title:
Analysis of Soybean GmPM31 Bioinformatics,Tissue Expression and Response to High Temperature and High Humidity
Author(s):
LIU Su-shuang1QIU Ying-sheng1LIU Yan-min1LI Yang2WEI Jia-ping3SHEN Ying-zi4WU Chou-fei2
(1.Institute of Science and Technology,Huzhou College,Huzhou 313000,China; 2.School of Life Sciences,Huzhou University,Huzhou 313000,China; 3.College of Agriculture,Gansu Agricultural University/State Key Laboratory of Aridland Crop Biology,Lanzhou 730070,China; 4.College of Agriculture,Nanjing Agricultural University/State Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing 210095,China)
Keywords:
Spring soybeanGmPM31Bioinformatics analysisCis-elementExpressionSeedHigh temperature and high humidity stress
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2021.05.0612
Abstract:
GmPM31 contains a typical ACD domain of the sHSPs family.In order to predict and study the function of GmPM31,we isolated the sequence of GmPM31 and its promoter from spring field deterioration resistant soybean Xiangdou 3,and resistance the structure and cis-regulatory element of GmPM31 by bioinformatics.In addition,we analyzed the tissue expression pattern and the expression levels of GmPM31 under high temperature and high humidity by qRT-PCR.The results showed that the complete ORF of GmPM31 was 459 bp.GmPM31 encoded 153 amino acids and contained a highly conserved ACD domain.The results of the evolutionary tree showed that GmPM31 has high homology with the Class I sHSPs,so we speculated GmPM31 may belong to the Class I sHSPs family.The prediction result of promoter cis-elements showed that the GmPM31 promoter contained many stress responsive elements such as hormone responsive element ABRE,ERE,AAGAA-motif,element involoved in MYB and MYC transcription factors related to drought and cold stress,high salt stress responsive element Box III,and anaerobic stress responsive element ARE.qRT-PCR results showed that the highest expression level of GmPM31 was found in mature seeds,followed by in young pods and leaves,and a lowest expression level in root,stems,leaves and flowers.Over the seed developing,the expression of GmPM31 increased at first and then decreased,and reached higher levels at 45 d after flowering.Compared to the corresponding control,the expression of GmPM31 in seeds was significantly higher at 96 and 168 h under high temperature and high humidity stress,which indicated that GmPM31 in seeds was up-regulated by high temperature and high humidity stress.These results showed that GmPM31 was involved in the development of spring soybean seeds and the response to high temperature and high humidity stress.

References:

[1]周亚丽,朱雅婧,赵飞云,等.大豆GmLEA的分离及其在种子活力中的功能分析[J].中国农业科学,2018,51(23):4397-4408.(Zhou Y L,Zhu Y J,Zhao F Y,et al.Isolation and functional analysis of soybean GmLEA in seed vigor[J].Scientia Agricultura Sinica,2018,51(23):4397-4408.)[2]Liu S S,Jia Y H,Zhu Y J,et al.Soybean matrix metalloproteinase Gm2-MMP relates to growth and development and confers enhanced tolerance to high temperature and humidity stress in transgenic Arabidopsis[J].Plant Molecular Biology Reporter,2018,36(1):94-106.[3]王芳,王丽群,田鑫,等.中国南方春大豆收获前后种子劣变的抗性研究[J].中国农业科学,2007,40(11):2637-2647.(Wang F,Wang L Q,Tian X,et al.Pre-harvest and post-harvest seed deterioration resistance of spring soybean germplasm in South China[J].Scientia Agricultura Sinica,2007,40(11):2637-2647.)[4]Liu S S,Jia Y H,Zhu Y J,et al.Soybean matrix metalloproteinase Gm2-MMP relates to growth and development and confers enhanced tolerance to high temperature and humidity stress in transgenic Arabidopsis[J].Plant Molecular Biology Reporter,2018,36(1):94-106.[5]Chow T Y,Lin T Y,Hsing Y C.A soybean seed maturation protein cDNA GmPM31 (accession No.AF117885) encode a class I low molecular weight heat shock protein.(PGR99-177)[J].Plant Physiology,1999,121(4):1383.[6]张宁,姜晶.植物中小分子热激蛋白基因家族(sHSPs)研究进展[J].植物生理学报,2017,53(6):943-948.(Zhang N,Jiang J.Research advances of small heat shock protein gene family (sHSPs) in plants[J].Plant Physiology Journal,2017,53(6):943-948.)[7]王敏,江彪,林毓娥,等.小分子热激蛋白参与植物抗逆性方面的研究进展[J].安徽农业科学,2018,46(18):29-32,35.(Wang M,Jiang B,Lin Y E,et al.Advances of small heat shock proteins participating in plant resistance[J].Journal of Anhui Agricultural Sciences,2018,46(18):29-32,35.)[8]Basha E,O’Neill H,Vierling E.Small heat shock proteins and α-crystallins:Dynamic proteins with flexible functions[J].Trends in Biochemical Sciences,2012,37(3):106-117.[9]Sun W N,Vanmontagu M,Verbruggen N.Small heat shock proteins and stress tolerance in plants[J].Biochimica et Biophysica Acta,2002,1577(1):1-9.[10]Wang W Q,Ye J Q,Rogowska-wrzesinska A,et al.Proteomic comparison between maturation drying and prematurely imposed drying of zea mays seeds reveals a potential role of maturation drying in preparing proteins for seed germination,seedling vigor,and pathogen resistance[J].Journal of Proteome Research,2014,13(2):606-626. [11]Arce D P,Krsticevic F J,Bertolaccini M R,et al.Analysis of small heat shock protein gene family expression (RNA-seq) during the tomato fruit maturation[J].IFMBE Proceedings,2015,49(3):679-682.[12]Zhang N,Shi J,Zhao H,et al.Activation of small heat shock protein (SlHSP17.7) gene by cell wall invertase inhibitor (SlCIF1) gene involved in sugar metabolism in tomato[J].Gene,2018,679:90-99.[13]李春梅.大豆种子蛋白的差异蛋白质组研究[D].南京:南京农业大学,2007.(Li C M.Comparative proteomic analysis on soybean seed protein[D].Nanjing:Nanjing Agricultural Univ-ersity,2007.)[14]Lopescaitar V S,Carvalho M C D,Darben L M,et al.Genome-wide analysis of the Hsp20 gene family in soybean:Comprehensive sequence,genomic organization and expression profile analysis under abiotic and biotic stresses[J].BMC Genomics,2013,14:577.[15]Yu X M,Jin H X,Fu X J,et al.Quantitative proteomic analyses of two soybean low phytic acid mutants to identify the genes associated with seed field emergence[J].BMC Plant Biology,2019,19(1):569.[16]Wang S,Tao Y,Zhou Y L,et al.Translationally controlled tumor protein GmTCTP interacts with GmCDPKSK5 in response to high temperature and humidity stress during soybean seed development[J].Plant Growth Regulation,2017,82(1):187-200.[17]俞佳虹,冯坤,程远,等.植物小热激蛋白的研究进展[J].分子植物育种,2017,15(8):3016-3023.(Yu J H,Feng K,Cheng Y,et al.Research progress on small heat shock proteins in plants[J].Molecular Plant Breeding,2017,15(8):3016-3023.)[18]McHaourab H S,Godar J A,Stewart P L.Structure and mechanism of protein stability sensors:Chaperone activity of small heat shock proteins[J].Biochemistry,2009,48(18):3828-3837.[19]Ma C,Haslbeck M,Babujee L,et al.Identification and chara-cterization of a stress-inducible and a constitutive small heat-shock protein targeted to the matrix of plant peroxisomes[J].Plant Physiology,2006,141:47-60.[20]Hernandez-Garcia C M,Finer J J.Identification and validation of promoters and cis-acting regulatory elements[J].Plant Science,2014,217-218:109-119.[21]冯玉芹.过表达内质网小分子热激蛋白对拟南芥抗逆性的影响[D].济南:山东师范大学,2010.(Feng Y Q.The study of the transgenic Arabidopsis with overexpression of endoplasmic reticulum small heat shock protein[D].Jinan:Shandong Normal University,2010.)[22]Chen X,Lin S,Liu Q,et al.Expression and interaction of small heat shock proteins (sHsps) in rice in response to heat stress[J].Biochimica et Biophysica Acta,2014,1844(4):818-828.[23]Neelam K S,Sachin K,Manu A,et al.Silencing of class I small heat shock proteins affects seed related attributes and thermo-tolerance in rice seedlings[J].Planta,2020,251:26.[24]Sedaghatmehr M,Mueller-Roeber B,Balazadeh S.The plastid metalloprotease FtsH6 and small heat shock protein HSP21 jointly regulate thermomemory in Arabidopsis[J].Nature Communications,2016,7:12439.[25]Sanchez-Bel P,Egea I,Sanchez-Ballesta M T,et al.Proteome changes in tomato fruits prior to visible symptoms of chilling injury are linked to defensive mmechanisms,uncoupling of photosynthetic processes and protein degradation machinery[J].Plant Cell Physiology,2012,53(2):470-484.[26]Zhang J,Chen H,Wang H,et al.Constitutive expression of a tomato small heat shock protein gene LeHSP21 improves tolerance to high-temperature stress by enhancing antioxidation capacity in tobacco[J].Plant Molecular Biology Report,2016,34(2):399-409.[27]Wang L Q,Ma H,Song L R,et al.Comparative proteomics analysis reveals the mechanism of pre-harvest seed deterioration of soybean under high temperature and humidity stress[J].Journal of Proteomics,2012,75(7):2109-2127.[28]Tao Y,Chen M,Shu Y J,et al.Identification and functional characterization of a novel BEL1-LIKE homeobox transcription factor GmBLH4 in soybean[J].Plant Cell,Tissue and Organ Culture,2018,134(2):331-344.

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Last Update: 2021-09-27