|Table of Contents|

Comparation on the Effects of Soy Isoflavones and Vitamin E on Hepatic Antioxidant Capacity of Aging Mice(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2020年05期
Page:
797-803
Research Field:
Publishing date:

Info

Title:
Comparation on the Effects of Soy Isoflavones and Vitamin E on Hepatic Antioxidant Capacity of Aging Mice
Author(s):
LIU Si-tong1;  LIU Ya-hui1;  YIN Xue-zhe2;  JIN Ming1
(1.Medical College, Yanbian University, Yanji 133002, China； 2.Yanbian University Hospital, Yanji 133000, China)
Keywords:
Soybean;  Isoflavones;  Anti-aging;  Antioxidant capacity;  D-galactose
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2020.05.0797
Abstract:
To compare the effects of soy isoflavones and vitamin E on hepatic antioxidant capacity of D-galactose-induced aging mice, we prepared the aging model mice by subcutaneous injection of D-galactose. The mice were randomly divided into 4 groups, including normal group, aging model group, vitamin E group and soy isoflavone group. Then the content of 8-hydroxy-2′-deoxyguanosine(8-OHdG) in serum was measured by ELISA. The activities of superoxide dismutase(SOD), glutathione peroxidase(GSH-Px), catalase(CAT), monoamine oxidase(MAO), ATPase, and content of malondialdehyde (MDA) in liver tissues were detected by the colorimetric method. The protein expression of Nrf2 and HO-1 were detected with western blotting assay. The pathological changes of liver tissues were detected with hematoxylin-eosin(HE) staining. The results showed that soy isoflavones and vitamin E decreased 8-OHdG content in serum significantly. Soy isoflavones and vitamin E increased the activities of SOD, GSH-Px, CAT and mitochondrial ATPase significantly, decreased MAO activity and MDA content, and up-regulated the protein expression of Nrf2 and HO-1 in aging mice significantly. In conclusion, soy isoflavones and vitamin E alleviated structure damages of liver cells during aging. Except for SOD, MAO and MDA level, there was no significant difference in other indexes between soy isoflavone and vitamin E groups. Soy isoflavones and vitamin E could elevate the hepatic antioxidant capacity of aging mice induced by D-galactose, and the mechanism may be related to the regulation of Nrf2 and HO-1.

References:

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Last Update: 2020-10-21