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Effects of Sublethal Concentration of Two Insecticides on Three Detoxification Enzymes and Protein Content of Aphis glycines(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2020年05期
Page:
790-796
Research Field:
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Title:
Effects of Sublethal Concentration of Two Insecticides on Three Detoxification Enzymes and Protein Content of Aphis glycines
Author(s):
LU Bing-yu;  LI Shuang-yu;  DONG Shuang;  YANG Hong-jia;  CHEN Ya-ru;  LIU Yan;  WANG Xiao-yun;  FAN Dong
（College of Agriculture, Northeast Agricultural University, Harbin 150030, China）
Keywords:
Aphis glycines;  Imidacloprid;  Azadirachtin;  Detoxification enzyme;  Sublethal effect;  Protein content
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2020.05.0790
Abstract:
 In order to make clear the sublethal effect of imidacloprid and azadirachtin on soybean aphids, the adult soybean aphids（Aphis glycines）, were treated by leaf dipping method. The toxicity regression equations of the two insecticides and the concentration of LC10, LC20and LC40 were calculated. Soybean aphids were treated with the sublethal concentrations for 1, 12, 24 and 48 h. The activity of three main detoxifying enzymes and the soluble protein content were measured. The toxicity equation of imidacloprid was y=1.178 4x+10.702 3, and the equation of azadirachtin was y=1.632 3x+10.881 5. After treated by imidacloprid, the activity of MFO was firstly inhibited and then induced, among which LC20 treatment had the strongest induction effect. Imidacloprid was mainly inhibitory to CES. The induction effect of imidacloprid LC10 treatment on GST activity was the strongest. Azadirachtin mainly inhibited the activity of the three enzymes, and only induced the activity of MFO and CES at 24 h. After treated with imidacloprid for 1 h, the soluble protein content of soybean aphid was significantly higher than that of CK. After treated by azadirachtin, the protein content was significantly higher than that of CK at 1 h, and the protein content of LC20 treatment was still significantly higher than that of CK at 12 h, with the concentration effect of increasing protein content first and then decreasing with the increasing of insecticide concentration. The results indicated that MFO and GST played key roles during the metabolism of imidacloprid by soybean aphid, but CES had no significant effect. In azadirachtin metabolism, MFO and CES only played a certain role in low concentration (LC10) treatment, GST was weak at all treatments. The soluble protein content of soybean aphid treated by the two insecticides was higher at 1 h.

References:

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Last Update: 2020-10-21