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Title:: Bioinformatics Analysis and Interacting Protein Prediction of Soybean bZIP Gene Glyma04g04170 in Response to Submergence Stress

Author(s):: LIN Yan-hui1; TANG Li-qiong1; XU Jing1; ZHU Hong-lin1; WANG Xin-hua1; WANG Min-fen1; XU Ran2; WANG Xiao-ning1; (1.Institute of Food Crops, Hainan Academy of Agricultural Sciences/Hainan Key Laboratory of Crop Genetics and Breeding/Hainan Scientific Research Station of Crop Gene Resource and Germplasm Enhancement, Ministry of Agriculture, Haikou 571100, China; 2.Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China)

Keywords:: Soybean; Submergence tolerance; Transcriptome; bZIP transcription factor

PACS:: -

DOI:: 10.11861/j.issn.1000-9841.2020.05.0727

Abstract:: bZIP transcription factors are involved in a variety of abiotic stresses, in order to study the regulation of bZIP transcription factors in soybean under submergence stress, we flooded the submergence-resistant soybean variety Qihuang 34, and performed transcriptome sequencing on the root tissue samples treated at different times, we finally selected a bZIP transcription factor encoding gene Glyma04g04170 as the focus of this research. The result of qRT-PCR analysis of Glyma04g04170 was consistent with the trend of transcriptome data. It suggested that Glyma04g04170 may response to submergence tolerance through negative regulation. The conserved domain of Glyma04g04170 protein was analyzed, and it was found that the protein contained a bZIP conserved domain. The tertiary structure of the protein showed the Leu’s position of leucine zipper involved in oligomerization in the conserved domain, and the N-x7-R/K structure which was combined with the specific DNA sequence and acted as a nuclear localization signal. In this study, Glyma04g04170 was isolated and the encoded protein was identified as a bZIP transcription factor binding with AREB/ABF. The proteins which interacted with Glyma04g04170 were predicted, and they were mainly serine/threonine acid residue protein phosphatase. The expression of Glyma02g37090 gene was significantly different in transcriptome data and qRT-PCR, and the expression levels in submergence treatment were increased compared with the control group at four time points.

References:

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Bioinformatics Analysis and Interacting Protein Prediction of Soybean bZIP Gene Glyma04g04170 in Response to Submergence Stress(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

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