|Table of Contents|

Screening and Application of Suitable Symbiotic Combination Between Rhizobia and Soybean Cultivar Xudou 24(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2020年04期
Page:
612-620
Research Field:
Publishing date:

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Title:
Screening and Application of Suitable Symbiotic Combination Between Rhizobia and Soybean Cultivar Xudou 24
Author(s):
LI Yan-lian1;  WANG Chuan-lei2;  XU Bao-min3;  ZHANG Xiu-guo4;  WEI Xiang-sheng5;  YANG Sheng-hui4; 5
(1.Department of Life Science and Engineering, Jining University, Qufu 273155, China; 2.Jining Rencheng Bureau of Science and Technology, Jining 272000, China; 3.Jining Bureau of Agriculture and Rural Affairs, Jining 272000, China; 4.College of Plant Protection, Shandong Agricultural University, Tai′an 271000, China; 5.Shandong Aifudi Biotech. Co., LTD., Jining 272000, China)
Keywords:
Xudou 24;  Huanghuaihai;  Rhizobia;  Symbiotic matching;  Yield;  Quality
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2020.04.0612
Abstract:
In order to study the symbiotic matching between the local soybean cultivar and rhizobial species, the main soybean variety Xudou 24 cultured in Huanghuaihai area was used in this experiment to trap rhizobia in the fields. We screened high efficient rhizobium strains matched with Xudou 24 symbiosis and studied the effects of fertilization and inoculation with efficient rhizobia on yield and quality of Xudou 24 by inoculated experiment in field. A total of 32 rhizobial strains were isolated from the trapping plants. These strains were identified as three populations belonging to the species Sinorhibizobium fredii, Bradyrhizobium elkanii and Bradyrhizobium japonicum by rpoB gene sequence comparison, in which S.fredii was the dominant with 75% of nodule occupation. Through the symbiotic matching screening,strain S.fredii 3 was verified as a high-efficiency symbiont of Xudou 24. The field inoculation experiments further proved that the inoculation of S.fredii 3 could improve the growth and yield of Xudou 24 and the number of nodules, fresh weight of nodules, yield and protein content of the plant in M2 treatment (base fertilizer, inoculation with S.fredii 3, non-topdressing) were the highest.Further analysis found that the number of nodules and fresh weight of nodules were positively correlated with the yield and protein content. Among them, the fresh weight of nodules and yield reached a significant positive correlation. The content of soil available boron(AB) was the most related to the community distribution of S.fredii, and the content of available iron(AFe) and available molybdenum(AMo) were the most important physicochemical factors affecting the community structure distribution of B.elkanii. It is showed that S.fredii 3 could be a suitable candidate for inoculant of Xudou 24 in the Huanghuaihai area.

References:

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Last Update: 2020-09-02