|Table of Contents|

Virulence and Control Effect of Parasitic Fungi on Race 3 of Soybean Cyst Nematode(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2020年04期
Page:
595-604
Research Field:
Publishing date:

Info

Title:
Virulence and Control Effect of Parasitic Fungi on Race 3 of Soybean Cyst Nematode
Author(s):
XU Yan-li1;  LU Jian-cong1;  SONG Jie1; 2
(1.Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin 150081,China； 2.College of Foreign Languages, Harbin University of Commerce, Harbin 150028,China)
Keywords:
Soybean cyst nematode;  Fusarium spp.;  Verticillium chlamydosporium;  Paecilomyces lilacinus;  Fermentation filtrate;  Control effect
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2020.04.0595
Abstract:
In order to test the inhibition of Paecilomy ceslilacinus，Fusarium spp. and Verticillium chlamydosporium on SCN, the inhibition of their fermentation filtrates on cyst, egg hatching and mortality of the second instar larvae (J2) of SCN race 3 were tested under laboratory conditions. The results showed that the fermentation filtrates of 12 strains of Fusarium spp., V.chlamydosporium and P.ceslilacinus had inhibitory effects on various morphology of SCN. The fermentation filtrates inhibited cysts and egg hatching of SCN, and had a lethal effect on J2. The fermentation filtrates of the tested strain inhibited the cyst from 57.5% to 81.2%, The 5-, 10-, 20- and 50-fold dilutions of the fermentation filtrates also showed an inhibitory effect on cyst of SCN with inhibition rate of 23.0%-77.0%. The fermentation filtrates inhibited the hatching rate of eggs of SCN from 41.6% to 80.6%. The fermentation filtrates of 12 strains had a lethal effect on J2 of SCN. The fermentation filtrates of 83.0% strains had lethal toxicity on J2 of SCN when J2 was treated for 1 h, and the lethal effect reached more than 80.0% after 48 h of treatment among the tested strains, the fermentation filtrates the F-9 strain of Fusarium, P-E strain of P.ceslilacinus, and V-25 strain of V.chlamydosporium significantly inhibited soybean cyst nematodes. In pot experiments with these strains, fungus fermentation filtrates reduced the density of females, cysts and eggs of soybean roots and J2 in the roots of soybean. The fungus fermentation filtrates also promoted plant growth and increased fresh weight of soybeans.

References:

［1］Hartwig E E. Breeding broductive soybean cultivars resistant to the soybean cyst nematode for the southern United States［J］. Plant Disease, 1981, 65(4):303-305.［2］孙漫红, 刘杏忠. 连作土壤中大豆胞囊线虫种群数量减少的原因探讨［J］.植物病理学报, 2000, 30(4): 353-356. (Sun M H, Liu X Z. Suppressive soil of soybean cysts nematode in China［J］. Acta Phytopathologica Sinica, 2000, 30(4): 353-356.)［3］孙玉秋, 许艳丽, 李春杰, 等．作物轮作系统对土壤中大豆胞囊线虫胞囊量和单胞囊卵量的影响［J］. 农业系统科学与综合研究, 2011, 27(2) : 248-252. (Sun Y Q, Xu Y L, Li C J, et al. Influence of cropping rotation systems on the volume of cysts and eggs in a single-cyst of soybean cyst nematode［J］. System Science and Comprehensive Studies in Agriculture, 2011, 27(2):248-252.)［4］陈立杰, 段玉玺, 范圣长, 等. 大豆胞囊线虫病的生防因子研究进展［J］.西北农林科技大学学报, 2005, 33(S): 190-194. (Chen L J, Duan Y X, Fan S C, et al. Advances in antagonists of soybean cyst nematode［J］.Journal of Northwest Science-Technique University of Agricutural and Forestry (Nature Science Edition), 2005,33(S): 190-194.)［5］Chen Z X, Chen S Y, Dickson D W. Nematology Advances and perspectives, volume 2 nematode management and utilization［M］. Beijing: Tsinghua University Press, CABI Publishing, 2004: 979-1071.［6］宋洁, 许艳丽, 姚钦. 大豆胞囊线虫主要寄生真菌对大豆耕作系统的响应［J］. 大豆科学, 2016, 35(3): 461-467. (Song J, Xu Y L, Yao Q. Response of major parasitic fungi of soybean cyst nematode on different cultivation systems［J］. Soybean Science,2016,35(3):461-467.)［7］Song J, Li S X , Xu Y L, et al. Diversity of parasitic fungi from soybean cyst nematode associated with long-term continuous cropping of soybean in black soil［J］. Acta Agricultural Scandinavica, Section B— Soil and Plant Science,2016,66(5):432-442.［8］赵晓晖, 许艳丽．厚垣轮枝菌发酵液对大豆胞囊线虫的抑制作用［J］．大豆科技, 2011, 20(4)：20-23.(Zhao X H, Xu Y L. Inhibition of Verticillium chlamydosporium fermented filtrates on soybean cyst nematode ［J］. Soybean Science and Technology, 2011, 20(4)：20-23.)［9］Fitters P F L, Belder E, Den B E. A time-lapse technique to study the effect of fungal products on embryogenesis of nematode eggs ［J］. Mededelingen van de Faculteit Landbouw wet enschappen, Universiteit Gent,1993, 58(2B): 751-756.［10］孙漫红, 刘杏忠, 晋治波. 淡紫拟青霉对大豆胞囊线虫卵及2龄幼虫的影响［J］. 植物保护学报, 2002, 29 (1)：57- 61.(Sun M H, Liu X Z, Jin Z B. Effect Paecilomy ceslilacinus on egg hatching and juvenile mortality of Heterodera glycines［J］. Acta Phytopylacica Sinica, 2002, 29 (1)：57- 61.)［11］赵晓晖, 许艳丽. 镰刀菌发酵液对大豆胞囊线虫的抑制作用［J］. 大豆科学, 2011, 30(3): 468-470, 474.(Zhao X H, Xu Y L. Inhibition of Fusarium spp. fermented filtrates on soybean cyst nematode［J］. Soybean Science, 2011, 30(3): 468-470.)［12］钱洪利, 许艳丽, 孙玉秋,等. 明尼苏达被毛孢代谢物对大豆胞囊线虫二龄幼虫的影响［J］. 大豆科学, 2009,29(1):118-121.(Qian H L, Xu Y L, Sun Y Q, et al. Effects of Hirsutella Minnesotensis metabolites on soybean cyst nematode juvenile［J］.Soybean Science,2009,29(1):118-121.)［13］刘霆, 王莉, 段玉玺,等. Snf 907真菌代谢物对大豆胞囊线虫卵及二龄幼虫的影响［J］. 大豆科学, 2006,25(3):326-328.(Liu T, Wang L, Duan Y X, et al. Effects of Snf 907 fungal metabolites on egg hatching and juvenile mortality Heterodera glycine ［J］.Soybean Science, 2006,25(3):326-328.)［14］李永峰, 赵云和,段玉玺,等. 防治大豆胞囊线虫病的土壤真菌的筛选［J］. 土壤通报, 2006,37(4):772-775.(Li Y F, Zhao Y H, Duan Y X ,et al. Screening of soil fungi inhibiting soybean cyst nematode［J］.Chinese Journal of Soil Science, 2006,37(4):772-775.)［15］Eyal K,Maya O,Jaacov K,et al.Soil suppressiveness to Fusarium disease: Shifts in root microbiome associated with reduction of pathogen root colonization［J］. Phytopathology,2013,103(1):23-33.［16］鲁建聪, 许艳丽, 宋洁．土壤扰动对寄生真菌代谢物温室防治大豆胞囊线虫的影响［J］.大豆科学,2016,35（1):106-111.(Lu J C, Xu Y L, Song J. Effect of soil disturbance to the biological control of fermentation filtrate of parasitic fungi on soybean cyst nematode in glasshouse［J］. Soybean Science, 2016,35(1):106-111.)［17］Ruan W B,Zhan L l, Xiao W, et al. An improved method for quantification of Heterodera glycine in plant tissues［J］.Nematropica, 2012, 42(2):237-244.［18］范圣长, 段玉玺, 陈立杰. 大豆胞囊线虫胞囊内寄生真菌研究［J］. 大豆科学, 2004, 23(1): 71-74.(Fan S C,Duan Y X,Chen L J. The research on the cyst entoparasitic fungi of soybean cyst nematode ［J］. Soybean Science,2004,23(1):71-74.)［19］Chen S Y, Liu X Z. Control of the soybean cyst nematode by the fungi Hirsutella rhossiliensis and Hirsutella minnesotensis in greenhouse studies［J］. Biological Control, 2005(32): 208-219.［20］陈立杰, 梁文举, 段玉玺, 等．施用生防颗粒剂对大豆田土壤线虫生物多样性的影响［J］．大豆科学, 2003, 22(4)：253-256．(Chen L J, Duan Y X, Fan S C,et al. Advances in antagonists of soybean cyst nematode［J］．Soybean Science, 2003,22(4): 253-256.)［21］刘杏忠, 刘文敏, 张东升．定殖于大豆胞囊线虫的淡紫拟青霉生物学特性研究［J］.中国生物防治,1995,11(2):70-74.（Liu X Z, Zhang W M, Zhang D X. Biological characteristics of Paecilomyceslilacinus associated with soybean cyst nematode ［J］.Chinese Journal of Biological Control, 1995,11(2):70-74.)［22］张新德, 刘杏忠, 张运全, 等.食线虫真菌防治大豆胞囊线虫的初步研究［J］.生物防治通报, 1993, 9(2): 89-90. (Zhang X D, Liu X Z, Zhang Y Q, et al. Preliminary studies on the nematophagous fungi inhibiting soybean cyst nematode［J］.Chinese Journal of Biological Control,1993,9(2): 89-90.)

Memo

Memo:
-
Last Update: 2020-09-02