|Table of Contents|

Bioinformatics and Expression Analysis Under Stress of Soybean Glyma.05g22700.2 Gene(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2020年04期
Page:
543-548
Research Field:
Publishing date:

Info

Title:
Bioinformatics and Expression Analysis Under Stress of Soybean Glyma.05g22700.2 Gene
Author(s):
LIU Chen;  WEI Ya-ting;  YU Yue-hua;  NI Zhi-yong
（College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, China）
Keywords:
Soybean;  Glyma.05G222700.2;  Bioinformatics;  qRT-PCR;  Salt stress
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2020.04.0543
Abstract:
In order to study the function and principle of serine/threonine protein kinase encoded by Glyma.05g22700.2 in the process of abiotic stress resistance, and promote the development and utilization of soybean stress resistant candidate genes, this study carried out the homology sequence, protein structure, phylogenetic tree and transcriptome analysis of soybean Glyma.05G222700.2 gene by bioinformatics method, and analyzed the expression in different tissues of soybean with qRT-PCR under salt stress. The results showed that the coding region of this gene was 2 040 bp, encoding 697 amino acids, with predicted molecular weight of 656.54 kD and pI8.026. Multiple sequence alignment revealed that the Glyma.05G222700.2 protein contained a Pkinase domain. Phylogenetic tree analysis showed that the protein had high consistency with wild soybean, mucuna, and red bean. Transcriptome data showed that the Glyma.05G222700.2 gene was expressed in various tissues of soybean, among which the expression level was the highest in seeds and the lowest in roots. Fluorescence quantitative PCR results showed that the Glyma.05G222700.2 gene was expressed in hairy roots, stems, and leaves under salt stress, with the highest expression in the stem and the lowest expression in the leaves. In the hairy roots, the expression reached the maximum at 12 h, and decreased at 24 h. The expression level in the stem increased and reached the maximum at 24 h. The expression of the gene was not stable in the leaves, and it didn′t express in the leaves for 2 h under salt stress. The expression of the leaves reached the extreme value at 6 h under salt stress and was higher than that of the control, and the expression under salt stress was lower than that of the control at 12 and 24 h. It is inferred that this gene may play an important role in the resistance of soybean to salt stress.

References:

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Last Update: 2020-09-02