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¡¶´ó¶¹¿Æѧ¡·[ISSN:1000-9841/CN:23-1227/S]

Issue:

2020Äê04ÆÚ

Page:

518-526

Research Field:

Publishing date:

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Title:

Research on the Regulation Effect of Ribosomal Gene GmRPL12 on Low Sulfur Tolerance in Soybeanª¤

Author(s):

CHEN Yan-ning;  WU Zhi-yi;  YUAN Wen-jie;  KAN Gui-zhen;  HUANG Fang;  YU De-yue;  WANG Hui

(Soybean Research Institute of Nanjing Agricultural University/National Center for Soybean Improvement/Key Laboratory of Biology and Genetic Improvement of Soybean (Ministry of Agriculture)/National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing 210095, China)

Keywords:

Soybean;  Ribosomal;  Gene;  Sulfur;  GmRPL12

PACS:

-

DOI:

10.11861/j.issn.1000-9841.2020.04.0518

Abstract:

In order to investigate the regulation of ribosomal genes on soybean tolerance to low sulfur stress, this study cloned a soybean ribosomal gene GmRPL12 from the root of Kefeng 1. We analysised the gene structure and expression in different tissues under low sulfur stress.The gene overexpression vector and interference vector were transformed into hairy root of Kefeng 1 to obtain transgenic chimeras, and then we analysised the expression of the gene and the phenotype of the chimera plant.The results of sequence analysis showed that, the total length of the gene code region was 576 bp. There was a ribosomal protein L7/L12 C-terminal domain RPL12 in its predicted protein. The gene was highly expressed in roots and was induced by low sulfur, and showed different expression patterns in two materials. Soybean chimeras with gene overexpression (OE), RNA interference (Ri), and two empty vectors (OE-EV and Ri-EV) were obtained by genetic transformation of hairy roots. Compared with that under the +S condition, SPAD value, the plant height, the fresh weight and dry weight of aboveground, the fresh weight and dry weight of root increased significantly in the GmRPL12 gene-overexpressed soybean chimera under the -S condition, and these indicators decreased significantly in the soybean Ri chimera under the -S condition. Furthermore, the inorganic sulfur contents of soybean OE chimeras increased significantly in the above ground and under ground plant under -S, and in the above ground plant under +S. These results suggest that GmRPL12 gene may be involved in soybean tolerance to low sulfur and soybean sulfur metabolism.

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Last Update: 2020-09-02